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Protein

UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase

Gene

murE

Organism
Mycobacterium leprae 3125609
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Catalytic activityi

ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei57UDP-MurNAc-L-Ala-D-Glu; via carbonyl oxygenUniRule annotation1
Binding sitei213UDP-MurNAc-L-Ala-D-GluUniRule annotation1
Binding sitei221UDP-MurNAc-L-Ala-D-GluUniRule annotation1
Binding sitei415Meso-diaminopimelateUniRule annotation1
Binding sitei493Meso-diaminopimelate; via carbonyl oxygenUniRule annotation1
Binding sitei497Meso-diaminopimelateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi144 – 150ATPUniRule annotation7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigaseUniRule annotationSAAS annotationImported
Biological processCell cycle, Cell divisionUniRule annotationSAAS annotation, Cell shape, Cell wall biogenesis/degradationUniRule annotationSAAS annotation, Peptidoglycan synthesisUniRule annotationSAAS annotation
LigandATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00219

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligaseUniRule annotation (EC:6.3.2.13UniRule annotation)
Alternative name(s):
Meso-A2pm-adding enzymeUniRule annotation
Meso-diaminopimelate-adding enzymeUniRule annotation
UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligaseUniRule annotation
UDP-MurNAc-tripeptide synthetaseUniRule annotation
UDP-N-acetylmuramyl-tripeptide synthetaseUniRule annotation
Gene namesi
Name:murEUniRule annotation
ORF Names:A8144_05280Imported
OrganismiMycobacterium leprae 3125609Imported
Taxonomic identifieri1297123 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000243179 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei253N6-carboxylysineUniRule annotation1

Post-translational modificationi

Carbamoylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP.UniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini52 – 130Mur_ligaseInterPro annotationAdd BLAST79
Domaini142 – 345Mur_ligase_MInterPro annotationAdd BLAST204
Domaini365 – 449Mur_ligase_CInterPro annotationAdd BLAST85

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni186 – 187UDP-MurNAc-L-Ala-D-Glu bindingUniRule annotation2
Regioni439 – 442Meso-diaminopimelate bindingUniRule annotation4

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi439 – 442Meso-diaminopimelate recognition motifUniRule annotation4

Sequence similaritiesi

Belongs to the MurCDEF family. MurE subfamily.UniRule annotationSAAS annotation

Family and domain databases

Gene3Di3.40.1190.10, 1 hit
3.90.190.20, 1 hit
HAMAPiMF_00208 MurE, 1 hit
InterProiView protein in InterPro
IPR036565 Mur-like_cat_sf
IPR004101 Mur_ligase_C
IPR036615 Mur_ligase_C_dom_sf
IPR013221 Mur_ligase_cen
IPR000713 Mur_ligase_N
IPR035911 MurE/MurF_N
IPR005761 UDP-N-AcMur-Glu-dNH2Pim_ligase
PfamiView protein in Pfam
PF01225 Mur_ligase, 1 hit
PF02875 Mur_ligase_C, 1 hit
PF08245 Mur_ligase_M, 1 hit
SUPFAMiSSF53244 SSF53244, 1 hit
SSF53623 SSF53623, 1 hit
SSF63418 SSF63418, 1 hit
TIGRFAMsiTIGR01085 murE, 1 hit

Sequencei

Sequence statusi: Complete.

A0A197SFA9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDGGVTEVV AVPVRLRPSA TAGVRLPELV AQVGAVLADG PGQAATVPDI
60 70 80 90 100
PVTGVTLRAQ EVLSGDLFAA LAGASTHGAR YAGVALERGA VAVLTDVAGV
110 120 130 140 150
AELTAQASSV PILIHPEPRS VLGGLAAAAY GHPSNRMTVV GITGTSGKTT
160 170 180 190 200
TTYMVEAGLR AGGRVVGLVG TIGIRIDGAD IPSFLTTPEA PALQAMLAAM
210 220 230 240 250
VERGVETVVM EVSSHALSLG RVEGTQFAVA GFTNLSRDHL DFHPDMEEYF
260 270 280 290 300
EAKAVLFDPH SLLRARTVVV CIDDDAGRAT AARAGDAITV SALGQPASWR
310 320 330 340 350
ATDIRSPGVG AQEFTAVDPA GLQHRVGIRL PGRYNVANCL VALAILDVVG
360 370 380 390 400
VSPEQASLGF RDIRIPGRLE RIDCGQDFLA LVDYAHKPGA LHSVLTALLQ
410 420 430 440 450
PDHRLAVVFG AGGERDPGKR APMGEIAAEL ADLVVVTDDN PRGEDPAAIR
460 470 480 490 500
RDILTGTVAA GGAAQVVEIG DRRAAIQYAV AWAGPDDVVL VAGKGHETGQ
510 520 530
RGAAETCPFD DRVELARALQ VRDARLLPAP GRACQ
Length:535
Mass (Da):55,250
Last modified:October 5, 2016 - v1
Checksum:iF26D9A5E1B144CC0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
LYPH01000011 Genomic DNA Translation: OAR21581.1

Similar proteinsi

Entry informationi

Entry nameiA0A197SFA9_MYCLR
AccessioniPrimary (citable) accession number: A0A197SFA9
Entry historyiIntegrated into UniProtKB/TrEMBL: October 5, 2016
Last sequence update: October 5, 2016
Last modified: June 20, 2018
This is version 14 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

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