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Entry version 32 (02 Jun 2021)
Sequence version 1 (05 Oct 2016)
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Protein

Gallate 1-beta-glucosyltransferase 84A23

Gene

UGT84A23

Organism
Punica granatum (Pomegranate)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Glucosyltransferase that catalyzes the formation of 1-O-beta-D-glucose esters with hydroxybenzoic acids and cinnamic acid including its derivatives as preferred glucosyl acceptors. Has significant activity with gallic acid (3,4,5-trihydroxybenzoic acid), 3,4-dihydroxybenzoic acid, 4-hydroxybenzoic acid, cinnamic acid, sinapic acid, coumaric acid, caffeic acid and ferulic acid in vitro. Gallic acid is the predicted native substrate of the enzyme, which thus catalyzes the formation of 1-O-galloyl-beta-D-glucose, the first committed step of hydrolyzable tannins (HTs) biosynthesis, with punicalagin isomers being the major HTs of pomegranate. Catalyzes the formation of flavonoid glucosides with genistein, apigenin and luteolin in vitro. Has low activity with benzoic acid, 2-hydroxybenzoic acid, 3-hydroxybenzoic acid, 2,4-dihydroxybenzoic acid, naringenin and quercetin. No activity with catechol, resveratrol, chlorogenic acid, catechin and epicatechin (building blocks of proanthocyanidins) or cyanidin, delphinidin and pelargonidin (the three anthocyanidins).

1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

kcat is 0.52 sec(-1) with gallic acid as substrate. kcat is 0.61 sec(-1) with 4-hydroxybenzoic acid as substrate. kcat is 0.76 sec(-1) with 3,4-dihydroxybenzoic acid as substrate. kcat is 0.64 sec(-1) with caffeic acid as substrate. kcat is 0.56 sec(-1) with cinnamic acid as substrate. kcat is 0.52 sec(-1) with coumaric acid as substrate. kcat is 0.72 sec(-1) with ferulic acid as substrate. kcat is 0.5 sec(-1) with sinapic acid as substrate.1 Publication
  1. KM=0.89 mM for gallic acid (at pH 5.0 and 30 degrees Celsius)1 Publication
  2. KM=1.19 mM for 4-hydroxybenzoic acid (at pH 5.0 and 30 degrees Celsius)1 Publication
  3. KM=2.46 mM for 3,4-dihydroxybenzoic acid (at pH 5.0 and 30 degrees Celsius)1 Publication
  4. KM=1.06 mM for caffeic acid (at pH 5.0 and 30 degrees Celsius)1 Publication
  5. KM=1.12 mM for cinnamic acid (at pH 5.0 and 30 degrees Celsius)1 Publication
  6. KM=0.94 mM for coumaric acid (at pH 5.0 and 30 degrees Celsius)1 Publication
  7. KM=1.58 mM for ferulic acid (at pH 5.0 and 30 degrees Celsius)1 Publication
  8. KM=0.86 mM for sinapic acid (at pH 5.0 and 30 degrees Celsius)1 Publication
  1. Vmax=0.37 µM/sec/mg enzyme with gallic acid as substrate (at pH 5.0 and 30 degrees Celsius)
  2. Vmax=0.43 µM/sec/mg enzyme with 4-hydroxybenzoic acid as substrate (at pH 5.0 and 30 degrees Celsius)
  3. Vmax=0.54 µM/sec/mg enzyme with 3,4-dihydroxybenzoic acid as substrate (at pH 5.0 and 30 degrees Celsius)
  4. Vmax=0.45 µM/sec/mg enzyme with caffeic acid as substrate (at pH 5.0 and 30 degrees Celsius)
  5. Vmax=0.4 µM/sec/mg enzyme with cinnamic acid as substrate (at pH 5.0 and 30 degrees Celsius)
  6. Vmax=0.37 µM/sec/mg enzyme with coumaric acid as substrate (at pH 5.0 and 30 degrees Celsius)
  7. Vmax=0.52 µM/sec/mg enzyme with ferulic acid as substrate (at pH 5.0 and 30 degrees Celsius)
  8. Vmax=0.35 µM/sec/mg enzyme with sinapic acid as substrate (at pH 5.0 and 30 degrees Celsius)

pH dependencei

Optimum pH is 5.1 Publication

Temperature dependencei

Activity remains fairly constant between 20-55 degrees Celsius. Active even at 0 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei284UDP-D-glucoseBy similarity1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionGlycosyltransferase, Transferase

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Gallate 1-beta-glucosyltransferase 84A23Curated (EC:2.4.1.1361 Publication)
Alternative name(s):
UDP-glucose:gallate glucosyltransferaseCurated
UDP-glycosyltransferase 84A231 Publication (EC:2.4.1.1201 Publication, EC:2.4.1.1701 Publication, EC:2.4.1.1771 Publication, EC:2.4.1.1941 Publication, EC:2.4.1.811 Publication)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:UGT84A231 PublicationImported
ORF Names:CDL15_Pgr003363Imported, CRG98_046055Imported
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiPunica granatum (Pomegranate)Imported
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri22663 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliopsidaeudicotyledonsGunneridaePentapetalaerosidsmalvidsMyrtalesLythraceaePunica
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000233551 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Unassembled WGS sequence
  • UP000197138 Componenti: Unassembled WGS sequence

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the 'Pathology and Biotech' section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

No difference in the levels of punicalagin in pomegranate hairy roots in a single RNAi knockdown of this gene. However, double RNAi knockdown of this gene together with UGT84A24 leads to significantly reduced levels of punicalagin and bis-hexahydroxydipheynyl glucose isomers, and to increased levels of galloyl glucosides (ether-linked gallic acid and glucose).1 Publication

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004523581 – 506Gallate 1-beta-glucosyltransferase 84A23Add BLAST506

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Expressed in roots of the seedlings.1 Publication

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
A0A193AUF6

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni342 – 343UDP-D-glucose bindingBy similarity2
Regioni360 – 368UDP-D-glucose bindingBy similarity9
Regioni382 – 385UDP-D-glucose bindingBy similarity4

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the UDP-glycosyltransferase family.UniRule annotation

Phylogenomic databases

Database of Orthologous Groups

More...
OrthoDBi
508327at2759

Family and domain databases

Conserved Domains Database

More...
CDDi
cd03784, GT1_Gtf-like, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR002213, UDP_glucos_trans
IPR035595, UDP_glycos_trans_CS

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00201, UDPGT, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00375, UDPGT, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

A0A193AUF6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGSESSLVHV FLVSFPGQGH VNPLLRLGKR LASKGLLVTF TTPESIGKQM
60 70 80 90 100
RKASNISDQP APVGDGFIRF EFFEDGWDED EPRRQDLDQY LPQLEKVGKV
110 120 130 140 150
LIPQMIQKNA EQGRPVSCLI NNPFIPWVSD VAETLGLPSA MLWVQSCACF
160 170 180 190 200
LAYYHYYHGL VPFPSENAME IDVQLPSMPL LKHDEVPSFL YPTTPYPFLR
210 220 230 240 250
RAILGQYKNL EKPFCILMDT FQELEHEIIE YTSKICPIKT VGPLFKNPKA
260 270 280 290 300
PNTTVKGDFM KADDCIGWLD SKPASSVVYV SFGSVVYLKQ DQWDEIAYGL
310 320 330 340 350
LNSGVNFLWV MKPPHKDSGY TVLTLPEGFL EKAGDRGKVV QWSPQEQVLA
360 370 380 390 400
HPATACFVTH CGWNSSMEAL TSGMPVVAFP QWGDQVTDAK YLVDEFKVGV
410 420 430 440 450
RMCRGEAEDK LITRDVVEQC LREATQGPKA AEMKKNALKW KAAAEASFVE
460 470 480 490 500
GGSSDRNLQA FVDEVKRRSI EITASKPAVK AAPNGVVAAA ESVVETKANG

KVELAA
Length:506
Mass (Da):56,129
Last modified:October 5, 2016 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i5F31597BA6DABD44
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
KT159805 mRNA Translation: ANN02875.1
MTKT01002492 Genomic DNA Translation: OWM79192.1
PGOL01006560 Genomic DNA Translation: PKI33499.1

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
KT159805 mRNA Translation: ANN02875.1
MTKT01002492 Genomic DNA Translation: OWM79192.1
PGOL01006560 Genomic DNA Translation: PKI33499.1

3D structure databases

SMRiA0A193AUF6
ModBaseiSearch...

Phylogenomic databases

OrthoDBi508327at2759

Family and domain databases

CDDicd03784, GT1_Gtf-like, 1 hit
InterProiView protein in InterPro
IPR002213, UDP_glucos_trans
IPR035595, UDP_glycos_trans_CS
PfamiView protein in Pfam
PF00201, UDPGT, 1 hit
PROSITEiView protein in PROSITE
PS00375, UDPGT, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiGGT23_PUNGR
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: A0A193AUF6
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 7, 2021
Last sequence update: October 5, 2016
Last modified: June 2, 2021
This is version 32 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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