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Protein

Lipoyl synthase, mitochondrial

Gene

VC83_02401

Organism
Pseudogymnoascus destructans
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N6-(octanoyl)lysine + an [Fe-S] cluster scaffold protein carrying a [4Fe-4S]2+ cluster + 2 S-adenosyl-L-methionine + 2 oxidized [2Fe-2S] ferredoxin + 6 H+ = protein N6-(dihydrolipoyl)lysine + an [Fe-S] cluster scaffold protein + 2 sulfide + 4 Fe3+ + 2 L-methionine + 2 5'-deoxyadenosine + 2 reduced [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi: protein lipoylation via endogenous pathway

This protein is involved in step 2 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Lipoyl synthase, mitochondrial (VC83_02401)
This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi134Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi139Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi145Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi165Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi169Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi172Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferaseUniRule annotation
Ligand4Fe-4SUniRule annotation, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionineUniRule annotation

Enzyme and pathway databases

UniPathwayi
UPA00538;UER00593

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lipoate synthaseUniRule annotation
Short name:
LSUniRule annotation
Short name:
Lip-synUniRule annotation
Lipoic acid synthaseUniRule annotation
Gene namesi
ORF Names:VC83_02401Imported
OrganismiPseudogymnoascus destructansImported
Taxonomic identifieri655981 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaLeotiomycetesLeotiomycetes incertae sedisPseudeurotiaceaePseudogymnoascus
Proteomesi
  • UP000077154 Componenti: Unassembled WGS sequence

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

MitochondrionUniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini155 – 377Elp3InterPro annotationAdd BLAST223

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00206 Lipoyl_synth, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006638 Elp3/MiaB/NifB
IPR031691 LIAS_N
IPR003698 Lipoyl_synth
IPR007197 rSAM
PfamiView protein in Pfam
PF16881 LIAS_N, 1 hit
PF04055 Radical_SAM, 1 hit
SFLDiSFLDF00271 lipoyl_synthase, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00510 lipA, 1 hit

Sequencei

Sequence statusi: Complete.

A0A177AIP6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MYATASCSRF RCLNAPPLRN AAKAVYINSS VRGLATPAGE TPATDTTTSA
60 70 80 90 100
AKPKGRPTYF KDTPVPTLDE YLDIDQPLTP AEMLALRPRT AKVGKKEITR
110 120 130 140 150
LPSWLKTSIP SPNTSAGFKA IKADLRGLGL HTVCEEARCP NIGECWGGSD
160 170 180 190 200
KASATATIML MGDTCTRGCR FCSVKTARKA KGAWDLAPLD PHEPENTAEA
210 220 230 240 250
LRRWGLGYVV LTSVDRDDLL DGGAAHFAET IAKIKSKSRE GGGREILVEA
260 270 280 290 300
LTGDFAGKLE MVDKVALSGL DVYAHNVETV DELTPQVRDR RATFAQSLKV
310 320 330 340 350
LGRAKEAVPS LITKTSIMLG LGETEPQLMD ALRRLREVNV DVVTFGQYMR
360 370 380 390 400
PTKRHMQVEE YISPQVFEAW RKRALEMGFL YCAAGPLVRS SYKAGEAFIE
410 420
NVLKQRKKGV EGVKKGEKEV VVEV
Length:424
Mass (Da):46,317
Last modified:September 7, 2016 - v1
Checksum:iE20DF0A7D0404920
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
KV441390 Genomic DNA Translation: OAF61044.1

Genome annotation databases

EnsemblFungiiOAF61044; OAF61044; VC83_02401

Similar proteinsi

Entry informationi

Entry nameiA0A177AIP6_9PEZI
AccessioniPrimary (citable) accession number: A0A177AIP6
Entry historyiIntegrated into UniProtKB/TrEMBL: September 7, 2016
Last sequence update: September 7, 2016
Last modified: July 18, 2018
This is version 10 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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