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Protein

DNA topoisomerase 3

Gene

topB_1

Organism
Enterobacter cloacae
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds two Mg2+ per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi19Magnesium 1; catalyticUniRule annotation1
Metal bindingi115Magnesium 1; catalyticUniRule annotation1
Metal bindingi115Magnesium 2UniRule annotation1
Metal bindingi117Magnesium 2UniRule annotation1
Active sitei340O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-bindingUniRule annotation, Isomerase, TopoisomeraseUniRule annotation
LigandMagnesiumUniRule annotation, Metal-bindingUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 3UniRule annotation (EC:5.99.1.2UniRule annotation)
Alternative name(s):
DNA topoisomerase IIIUniRule annotation
Gene namesi
Name:topB_1Imported
Synonyms:topBUniRule annotation
ORF Names:SAMEA2273167_02937Imported
OrganismiEnterobacter cloacaeImported
Taxonomic identifieri550 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEnterobacterEnterobacter cloacae complex
Proteomesi
  • UP000077423 Componenti: Unassembled WGS sequence

Interactioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei73Interaction with DNAUniRule annotation1
Sitei182Interaction with DNAUniRule annotation1
Sitei190Interaction with DNAUniRule annotation1
Sitei197Interaction with DNAUniRule annotation1
Sitei342Interaction with DNAUniRule annotation1

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 146ToprimInterPro annotationAdd BLAST134

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni206 – 211Interaction with DNAUniRule annotation6

Sequence similaritiesi

Belongs to the type IA topoisomerase family.UniRule annotation

Family and domain databases

CDDicd00186 TOP1Ac, 1 hit
cd03362 TOPRIM_TopoIA_TopoIII, 1 hit
Gene3Di1.10.290.10, 1 hit
1.10.460.10, 2 hits
2.70.20.10, 2 hits
HAMAPiMF_00953 Topoisom_3_prok, 1 hit
InterProiView protein in InterPro
IPR000380 Topo_IA
IPR003601 Topo_IA_2
IPR023406 Topo_IA_AS
IPR013497 Topo_IA_cen
IPR013824 Topo_IA_cen_sub1
IPR013825 Topo_IA_cen_sub2
IPR013826 Topo_IA_cen_sub3
IPR023405 Topo_IA_core_domain
IPR003602 Topo_IA_DNA-bd_dom
IPR005738 TopoIII
IPR006171 TOPRIM_domain
IPR034144 TOPRIM_TopoIII
PANTHERiPTHR11390 PTHR11390, 1 hit
PfamiView protein in Pfam
PF01131 Topoisom_bac, 1 hit
PF01751 Toprim, 1 hit
PRINTSiPR00417 PRTPISMRASEI
SMARTiView protein in SMART
SM00437 TOP1Ac, 1 hit
SM00436 TOP1Bc, 1 hit
SM00493 TOPRIM, 1 hit
SUPFAMiSSF56712 SSF56712, 1 hit
TIGRFAMsiTIGR01056 topB, 1 hit
PROSITEiView protein in PROSITE
PS00396 TOPOISOMERASE_I_PROK, 1 hit
PS50880 TOPRIM, 1 hit

Sequencei

Sequence statusi: Complete.

A0A156F3L2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAVAPWLRFV NSMRLFIAEK PSLARAIADV LPKPHRKGDG FIECGNGQVV
60 70 80 90 100
TWCIGHLLEQ AQPDAYDSRY ARWSLVDLPI VPEKWQLQPR PSVTKQLNVI
110 120 130 140 150
KRFLHEASEV IHAGDPDREG QLLVDEVLDY LQLAPEKRQK VQRCLINDLN
160 170 180 190 200
PQAVERAIER LRANSEFIPL CVSALARARA DWLYGINMTR AYTILGRNAG
210 220 230 240 250
YQGVLSVGRV QTPVLGLVVR RDEEIENFVA KDFFEVKAHI VTPADERFTA
260 270 280 290 300
IWQPSEACEP YQDEEGRLLN RTLAEHVVNR INGQPAIVTS YNDKRESESA
310 320 330 340 350
PLPFSLSALQ IEAAKRFGLS AQNVLDICQK LYETHKLITY PRSDSRYLPE
360 370 380 390 400
EHFAGRHAVL NAISVHAPDL LPQPAVNPDT RNRCWDDKKV DAHHAIIPTA
410 420 430 440 450
RSSSINLNDN EAKIYNLIAR QYLMQFCADA VFRKCVIELD IAKGKFVAKA
460 470 480 490 500
RFLAEAGWRT LLGNKERDED NDGTPLPVVA KDDELLCEKG EVVERQTQPP
510 520 530 540 550
RHFTDATLLS AMTGIARFVQ DKDLKKILRA TDGLGTEATR AGIIELLFKR
560 570 580 590 600
GFLVKKGRYI HSTDAGKALF HSLPEMATRP DMTAHWESIL TQISEKQCRY
610 620 630 640 650
QDFMQPLVGT LYQLIDQARS TPVRRFRGIA APGGSGSDKK KSAPRKRPAK
660
KSPPSEEAGL
Length:660
Mass (Da):74,251
Last modified:June 8, 2016 - v1
Checksum:iBCC8502330EE8806
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FKED01000010 Genomic DNA Translation: SAE11551.1

Genome annotation databases

EnsemblBacteriaiSAE11551; SAE11551; SAMEA2273167_02937

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FKED01000010 Genomic DNA Translation: SAE11551.1

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiSAE11551; SAE11551; SAMEA2273167_02937

Family and domain databases

CDDicd00186 TOP1Ac, 1 hit
cd03362 TOPRIM_TopoIA_TopoIII, 1 hit
Gene3Di1.10.290.10, 1 hit
1.10.460.10, 2 hits
2.70.20.10, 2 hits
HAMAPiMF_00953 Topoisom_3_prok, 1 hit
InterProiView protein in InterPro
IPR000380 Topo_IA
IPR003601 Topo_IA_2
IPR023406 Topo_IA_AS
IPR013497 Topo_IA_cen
IPR013824 Topo_IA_cen_sub1
IPR013825 Topo_IA_cen_sub2
IPR013826 Topo_IA_cen_sub3
IPR023405 Topo_IA_core_domain
IPR003602 Topo_IA_DNA-bd_dom
IPR005738 TopoIII
IPR006171 TOPRIM_domain
IPR034144 TOPRIM_TopoIII
PANTHERiPTHR11390 PTHR11390, 1 hit
PfamiView protein in Pfam
PF01131 Topoisom_bac, 1 hit
PF01751 Toprim, 1 hit
PRINTSiPR00417 PRTPISMRASEI
SMARTiView protein in SMART
SM00437 TOP1Ac, 1 hit
SM00436 TOP1Bc, 1 hit
SM00493 TOPRIM, 1 hit
SUPFAMiSSF56712 SSF56712, 1 hit
TIGRFAMsiTIGR01056 topB, 1 hit
PROSITEiView protein in PROSITE
PS00396 TOPOISOMERASE_I_PROK, 1 hit
PS50880 TOPRIM, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiA0A156F3L2_ENTCL
AccessioniPrimary (citable) accession number: A0A156F3L2
Entry historyiIntegrated into UniProtKB/TrEMBL: June 8, 2016
Last sequence update: June 8, 2016
Last modified: February 28, 2018
This is version 12 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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