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Protein

Deaminated glutathione amidase

Gene

ybeM

Organism
Escherichia coli (strain B / BL21-DE3)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes deaminated glutathione (dGSH) to 2-oxoglutarate and L-cysteinylglycine (specific activity 40.63 µmol/min/mg), very little activity against alpha-ketoglutaramate (a-KGM, specific activity 0.178 µmol/min/mg) and no activity on glutathione or L-glutamine. May function as a metabolite repair enzyme.1 Publication

Catalytic activityi

N-(4-oxoglutarate)-L-cysteinylglycine + H2O = 2-oxoglutarate + L-cysteinylglycine.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei40Proton acceptorPROSITE-ProRule annotation1
Active sitei110PROSITE-ProRule annotation1
Active sitei147NucleophilePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Deaminated glutathione amidase1 Publication (EC:3.5.1.-1 Publication)
Short name:
dGSH amidase1 Publication
Alternative name(s):
Nitrilase homolog 1
ecYbeM1 Publication
Gene namesi
Name:ybeM
Ordered Locus Names:ECBD_3025
OrganismiEscherichia coli (strain B / BL21-DE3)
Taxonomic identifieri469008 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000002032 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004406931 – 262Deaminated glutathione amidaseAdd BLAST262

Structurei

3D structure databases

ProteinModelPortaliA0A140NCB4
SMRiA0A140NCB4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 238CN hydrolasePROSITE-ProRule annotationAdd BLAST238

Sequence similaritiesi

Phylogenomic databases

KOiK11206
OMAiAAWVKGP

Family and domain databases

Gene3Di3.60.110.10, 1 hit
InterProiView protein in InterPro
IPR003010 C-N_Hydrolase
IPR036526 C-N_Hydrolase_sf
IPR001110 UPF0012_CS
PfamiView protein in Pfam
PF00795 CN_hydrolase, 1 hit
SUPFAMiSSF56317 SSF56317, 1 hit
PROSITEiView protein in PROSITE
PS50263 CN_HYDROLASE, 1 hit
PS01227 UPF0012, 1 hit

Sequencei

Sequence statusi: Complete.

A0A140NCB4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLVAAGQFAV TSVWEKNAEI CASLMAQAAE NDVSLFVLPE ALLARDDHDA
60 70 80 90 100
DLSVKSAQLL EGEFLGRLRR ESKRNMMTTI LTIHVPSTPG RAWNMLVALQ
110 120 130 140 150
AGNIVARYAK LHLYDAFAIQ ESRRVDAGNE IAPLLEVEGM KVGLMTCYDL
160 170 180 190 200
RFPELALAQA LQGAEILVLP AAWVRGPLKE HHWSTLLAAR ALDTTCYMVA
210 220 230 240 250
AGECGNKNIG QSRIIDPFGV TIAAASEMPA LIMAEVTPER VRQVRAQLPV
260
LNNRRFAPPQ LL
Length:262
Mass (Da):28,681
Last modified:May 11, 2016 - v1
Checksum:i74CF04FD3B9CC1DF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001665 Genomic DNA Translation: ACT30034.1
RefSeqiWP_000959109.1, NC_012971.2

Genome annotation databases

EnsemblBacteriaiACT30034; ACT30034; ECBD_3025
KEGGiebd:ECBD_3025
ebe:B21_00584
ebl:ECD_00595
PATRICifig|469008.15.peg.590

Similar proteinsi

Entry informationi

Entry nameiYBEM_ECOBD
AccessioniPrimary (citable) accession number: A0A140NCB4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2017
Last sequence update: May 11, 2016
Last modified: March 28, 2018
This is version 13 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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