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Protein

ATP-dependent RNA helicase DbpA

Gene

dbpA

Organism
Escherichia coli (strain B / BL21-DE3)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

DEAD-box RNA helicase involved in the assembly of the 50S ribosomal subunit. Has an RNA-dependent ATPase activity, which is specific for 23S rRNA, and a 3' to 5' RNA helicase activity that uses the energy of ATP hydrolysis to destabilize and unwind short rRNA duplexes.UniRule annotation

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHelicaseUniRule annotationImported, Hydrolase, RNA-bindingUniRule annotation
Biological processRibosome biogenesisUniRule annotation
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase DbpAUniRule annotation (EC:3.6.4.13UniRule annotation)
Gene namesi
Name:dbpAUniRule annotation
Ordered Locus Names:ECBD_2273Imported
OrganismiEscherichia coli (strain B / BL21-DE3)Imported
Taxonomic identifieri469008 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000002032 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA0A140NC60
SMRiA0A140NC60
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 31Q_MOTIFInterPro annotationAdd BLAST29
Domaini34 – 205Helicase ATP-bindingInterPro annotationAdd BLAST172
Domaini230 – 376Helicase C-terminalInterPro annotationAdd BLAST147

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni383 – 457Involved in 23S rRNA bindingUniRule annotationAdd BLAST75

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi3 – 31Q motifPROSITE-ProRule annotationAdd BLAST29

Domaini

Contains an N-terminal domain that binds non-specifically to RNA and a C-terminal domain that binds specifically and tightly to hairpin 92 of 23S rRNA.UniRule annotation

Sequence similaritiesi

Belongs to the DEAD box helicase family. DbpA subfamily.UniRule annotation

Phylogenomic databases

KOiK05591
OMAiFGCQALV

Family and domain databases

CDDicd00079 HELICc, 1 hit
Gene3Di3.30.70.330, 1 hit
HAMAPiMF_00965 DEAD_helicase_DbpA, 1 hit
InterProiView protein in InterPro
IPR005580 DbpA/CsdA_RNA-bd_dom
IPR011545 DEAD/DEAH_box_helicase_dom
IPR028619 DEAD_helicase_DbpA
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR027417 P-loop_NTPase
IPR000629 RNA-helicase_DEAD-box_CS
IPR014014 RNA_helicase_DEAD_Q_motif
PfamiView protein in Pfam
PF03880 DbpA, 1 hit
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00039 DEAD_ATP_HELICASE, 1 hit
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS51195 Q_MOTIF, 1 hit

Sequencei

Sequence statusi: Complete.

A0A140NC60-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAFSTLNVL PPAQLTNLNE LGYLTMTPVQ AAALPAILAG KDVRVQAKTG
60 70 80 90 100
SGKTAAFGLG LLQQIDASLF QTQALVLCPT RELADQVAGE LRRLARFLPN
110 120 130 140 150
TKILTLCGGQ PFGMQRDSLQ HAPHIIVATP GRLLDHLQKG TVSLDALNTL
160 170 180 190 200
VMDEADRMLD MGFSDAIDDV IRFAPASRQT LLFSATWPEA IAAISGRVQR
210 220 230 240 250
DPLAIEIDST DALPPIEQQF YETSSKGKIP LLQRLLSLHQ PSSCVVFCNT
260 270 280 290 300
KKDCQAVCDA LNEVGQSALS LHGDLEQRDR DQTLVRFANG SARVLVATDV
310 320 330 340 350
AARGLDIKSL ELVVNFELAW DPEVHVHRIG RTARAGNSGL AISFCAPEEA
360 370 380 390 400
QRANIISDML QIKLNWQTPP ANSSIATLEA EMATLCIDGG KKAKMRPGDV
410 420 430 440 450
LGALTGDIGL DGADIGKIAV HPAHVYVAVR QAVAHKAWKQ LQGGKIKGKT

CRVRLLK
Length:457
Mass (Da):49,188
Last modified:May 11, 2016 - v1
Checksum:iD9198B1FABA4D38A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001665 Genomic DNA Translation: ACT29301.1
RefSeqiWP_000123737.1, NC_012971.2

Genome annotation databases

EnsemblBacteriaiACT29301; ACT29301; ECBD_2273
KEGGiebd:ECBD_2273
ebe:B21_01330
ebl:ECD_01320
PATRICifig|469008.15.peg.1359

Similar proteinsi

Entry informationi

Entry nameiA0A140NC60_ECOBD
AccessioniPrimary (citable) accession number: A0A140NC60
Entry historyiIntegrated into UniProtKB/TrEMBL: May 11, 2016
Last sequence update: May 11, 2016
Last modified: March 28, 2018
This is version 14 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

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