Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Thymidylate synthase

Gene

thyA

Organism
Streptococcus suis
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotationSAAS annotation

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei156PROSITE-ProRule annotation1
Active sitei156NucleophileUniRule annotation1
Binding sitei1835,10-methylenetetrahydrofolateUniRule annotation1
Binding sitei191dUMPUniRule annotation1
Binding sitei2805,10-methylenetetrahydrofolate; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi135 – 136dUMP; shared with dimeric partnerUniRule annotation2
Nucleotide bindingi180 – 183dUMPUniRule annotation4
Nucleotide bindingi221 – 223dUMPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferaseUniRule annotationSAAS annotationImported, Transferase
Biological processNucleotide biosynthesisUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00575

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotationSAAS annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotationImported
ORF Names:ERS132431_00548Imported, ERS132531_00818Imported, ERS132539_01483Imported
OrganismiStreptococcus suisImported
Taxonomic identifieri1307 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
Proteomesi
  • UP000074903 Componenti: Unassembled WGS sequence
  • UP000071533 Componenti: Unassembled WGS sequence
  • UP000069526 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 281Thymidylat_syntInterPro annotationAdd BLAST272

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Family and domain databases

CDDicd00351 TS_Pyrimidine_HMase, 1 hit
Gene3Di3.30.572.10, 1 hit
HAMAPiMF_00008 Thymidy_synth_bact, 1 hit
InterProiView protein in InterPro
IPR023451 Thymidate_synth/dCMP_Mease
IPR036926 Thymidate_synth/dCMP_Mease_sf
IPR000398 Thymidylate_synthase
IPR020940 Thymidylate_synthase_AS
PfamiView protein in Pfam
PF00303 Thymidylat_synt, 1 hit
PRINTSiPR00108 THYMDSNTHASE
SUPFAMiSSF55831 SSF55831, 1 hit
PROSITEiView protein in PROSITE
PS00091 THYMIDYLATE_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

A0A0Z8I6N8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTMTKADIIF KENIRKIMEE GVFSENARPR YKDGNVANSK YITGSFAEYD
60 70 80 90 100
LAKGEFPITT LRPIPIKSAI KEILWIYQDQ TNDLSVLQEK YGVQYWNDWE
110 120 130 140 150
VEGTGTIGER YGAIVKKHDI ISKILKQLEE NPWNRRNVIS LWDYEAFDQS
160 170 180 190 200
AGLLPCAFQT MFDVRRVDGE IYLDATLTQR SNDMLVAHHI NAMQYVALQM
210 220 230 240 250
MIAKHFGWKV GKFFYFINNL HIYDNQFEQA EELLRRTPSE VEPRLVLNVP
260 270 280
DGTNFFDIRA EDFELVDYNP VKPQLKFDLA I
Length:281
Mass (Da):32,798
Last modified:April 13, 2016 - v1
Checksum:i1BB532EAB6A6B9D1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FIHS01000005 Genomic DNA Translation: CYV29893.1
FIJK01000036 Genomic DNA Translation: CYW39369.1
FILX01000009 Genomic DNA Translation: CYX52986.1

Genome annotation databases

EnsemblBacteriaiCYV29893; CYV29893; ERS132431_00548
CYW39369; CYW39369; ERS132539_01483
CYX52986; CYX52986; ERS132531_00818

Similar proteinsi

Entry informationi

Entry nameiA0A0Z8I6N8_STRSU
AccessioniPrimary (citable) accession number: A0A0Z8I6N8
Entry historyiIntegrated into UniProtKB/TrEMBL: April 13, 2016
Last sequence update: April 13, 2016
Last modified: February 28, 2018
This is version 14 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health