UniProtKB - A0A0X9ZXJ2 (A0A0X9ZXJ2_9ALPH)
Capsid scaffolding protein
ORF35
Functioni
Assemblin: Protease that plays an essential role in virion assembly within the nucleus. Catalyzes the cleavage of the assembly protein after formation of the spherical procapsid. By that cleavage, the capsid matures and gains its icosahedral shape. The cleavage sites seem to include -Ala-Ser-, -Ala-Ala-, as well as Ala-Thr bonds. Assemblin and cleavages products are evicted from the capsid before or during DNA packaging.
UniRule annotationAssembly protein: Plays a major role in capsid assembly. Acts as a scaffold protein by binding major capsid protein. Multimerizes in the nucleus such as major capsid protein forms the icosahedral T=16 capsid. Cleaved by assemblin after capsid completion. The cleavages products are evicted from the capsid before or during DNA packaging.
UniRule annotationCapsid scaffolding protein: Acts as a scaffold protein by binding major capsid protein in the cytoplasm, inducing the nuclear localization of both proteins. Multimerizes in the nucleus such as major capsid protein forms the icosahedral T=16 capsid. Autocatalytic cleavage releases the assembly protein, and subsequently abolishes interaction with major capsid protein. Cleavages products are evicted from the capsid before or during DNA packaging.
UniRule annotationCaution
Catalytic activityi
- Cleaves -Ala-|-Ser- and -Ala-|-Ala- bonds in the scaffold protein.UniRule annotation EC:3.4.21.97
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 55 | Charge relay systemUniRule annotation | 1 | |
Active sitei | 123 | Charge relay systemUniRule annotation | 1 | |
Active sitei | 142 | Charge relay systemUniRule annotation | 1 |
GO - Molecular functioni
- identical protein binding Source: UniProtKB-UniRule
- serine-type endopeptidase activity Source: UniProtKB-UniRule
GO - Biological processi
- viral release from host cell Source: UniProtKB-UniRule
Keywordsi
Molecular function | Hydrolase, Protease, Serine proteaseUniRule annotation |
Biological process | Viral capsid assemblyUniRule annotationARBA annotation, Viral release from host cell |
Names & Taxonomyi
Protein namesi | Recommended name: Capsid scaffolding proteinUniRule annotationAlternative name(s): Protease precursorUniRule annotation Short name: pPRUniRule annotation Cleaved into the following 2 chains: Alternative name(s): ProteaseUniRule annotation Assembly proteinUniRule annotation Alternative name(s): Capsid assembly proteinUniRule annotation |
Gene namesi | Name:ORF35Imported |
Organismi | Equid alphaherpesvirus 4 (Equine herpesvirus 4)Imported |
Taxonomic identifieri | 10331 [NCBI] |
Taxonomic lineagei | Viruses › Duplodnaviria › Heunggongvirae › Peploviricota › Herviviricetes › Herpesvirales › Herpesviridae › Alphaherpesvirinae › Varicellovirus |
Subcellular locationi
Other locations
- Host cytoplasm UniRule annotation
Other locations
- Host nucleus UniRule annotation
Other locations
- Host nucleus UniRule annotation
Other locations
- host cell cytoplasm Source: UniProtKB-SubCell
- host cell nucleus Source: UniProtKB-SubCell
Keywords - Cellular componenti
Host cytoplasmUniRule annotationARBA annotation, Host nucleusUniRule annotationPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_5033152047 | 1 – 647 | Capsid scaffolding proteinUniRule annotationAdd BLAST | 647 | |
ChainiPRO_5033152046 | 1 – 242 | AssemblinUniRule annotationAdd BLAST | 242 | |
ChainiPRO_5033152045 | 243 – 647 | Assembly proteinUniRule annotationAdd BLAST | 405 |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 242 – 243 | Cleavage; by assemblin; Release siteUniRule annotation | 2 |
Keywords - PTMi
PhosphoproteinUniRule annotationARBA annotationInteractioni
Subunit structurei
Capsid scaffolding protein homomultimerizes and interacts with major capsid protein. Assemblin exists in a monomer-dimer equilibrium with the dimer being the active species. Assembly protein homomultimerizes and interacts with major capsid protein.
UniRule annotationGO - Molecular functioni
- identical protein binding Source: UniProtKB-UniRule
Structurei
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 290 – 321 | DisorderedSequence analysisAdd BLAST | 32 | |
Regioni | 421 – 454 | DisorderedSequence analysisAdd BLAST | 34 | |
Regioni | 627 – 647 | Interaction with major capsid proteinUniRule annotationAdd BLAST | 21 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 290 – 306 | Polar residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 422 – 441 | Polar residuesSequence analysisAdd BLAST | 20 |
Domaini
Sequence similaritiesi
Family and domain databases
Gene3Di | 3.20.16.10, 1 hit |
HAMAPi | MF_04008, HSV_SCAF, 1 hit |
InterProi | View protein in InterPro IPR035443, Herpes_virus_sf IPR001847, Peptidase_S21 |
Pfami | View protein in Pfam PF00716, Peptidase_S21, 1 hit |
PRINTSi | PR00236, HSVCAPSIDP40 |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MDAYATDSNV VSLPIYVAGY IALYDMGDGG ELTLTRETVA AALPPASRLP
60 70 80 90 100
INIDHRNGCV VGEVLSIVDD VRGPFFLGIV NCPQLGAVLA TAAGPDFFGE
110 120 130 140 150
MSEGLSEQER LLYLVSNYLP SASLSSRRLG PDEEPDETLF AHVSLCVIGR
160 170 180 190 200
RVGTIVTYDA TPENTVAPFK RLSPSVREEL LITAREAQSR LGDMATWHVS
210 220 230 240 250
EDTLTRVLLS TAVNNMLLRN RWNLVARRRR EAGIEGHTYL QASASFGITN
260 270 280 290 300
GCNKADFCGA ELVGTCGYKS GERVRDISYS RVTLGAKAFT SSGSSALPSS
310 320 330 340 350
DNSAGQIGER AKHPGAMASS NPQTLSAAGA PLVSGDYILV PAAQYNQLVV
360 370 380 390 400
GQHTSHPPIN AGQASATHAV PTQYIQPAYN SMVPTSMYQA PPYWSVPPST
410 420 430 440 450
NLEAQITALV GALAADRKAA KSSDSQAVQN SQCSPPLSPQ QERRYTRKRR
460 470 480 490 500
HDWDANTRDD IEGIYYPGER SPRPVERRAG RASTTIADLM GAVSSLQQEV
510 520 530 540 550
SQLRAIQTVT AQQQVAPAGL YKPVPPGVPQ QYSQYQYIHP QHAVSAIISP
560 570 580 590 600
QLSGIQAQPP QSVIATPVPA VETPGGAKVC AAPTATHQAV EHSKAVQPQF
610 620 630 640
EAVTSAAVLP VNQPQASSQT VDASASTGLD FGRDDADIFV SQMMSAR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | KT324737 Genomic DNA Translation: AMB16074.1 KT324738 Genomic DNA Translation: AMB16153.1 KT324739 Genomic DNA Translation: AMB16232.1 KT324741 Genomic DNA Translation: AMB16390.1 KT324742 Genomic DNA Translation: AMB16469.1 LC075584 Genomic DNA Translation: BAV93321.1 LC075585 Genomic DNA Translation: BAV93400.1 LC075587 Genomic DNA Translation: BAV93558.1 LC075588 Genomic DNA Translation: BAV93637.1 |
RefSeqi | NP_045252.1, NC_001844.1 |
Genome annotation databases
GeneIDi | 1487600 |
KEGGi | vg:1487600 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | KT324737 Genomic DNA Translation: AMB16074.1 KT324738 Genomic DNA Translation: AMB16153.1 KT324739 Genomic DNA Translation: AMB16232.1 KT324741 Genomic DNA Translation: AMB16390.1 KT324742 Genomic DNA Translation: AMB16469.1 LC075584 Genomic DNA Translation: BAV93321.1 LC075585 Genomic DNA Translation: BAV93400.1 LC075587 Genomic DNA Translation: BAV93558.1 LC075588 Genomic DNA Translation: BAV93637.1 |
RefSeqi | NP_045252.1, NC_001844.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Genome annotation databases
GeneIDi | 1487600 |
KEGGi | vg:1487600 |
Family and domain databases
Gene3Di | 3.20.16.10, 1 hit |
HAMAPi | MF_04008, HSV_SCAF, 1 hit |
InterProi | View protein in InterPro IPR035443, Herpes_virus_sf IPR001847, Peptidase_S21 |
Pfami | View protein in Pfam PF00716, Peptidase_S21, 1 hit |
PRINTSi | PR00236, HSVCAPSIDP40 |
MobiDBi | Search... |
Entry informationi
Entry namei | A0A0X9ZXJ2_9ALPH | |
Accessioni | A0A0X9ZXJ2Primary (citable) accession number: A0A0X9ZXJ2 Secondary accession number(s): O39278 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | April 13, 2016 |
Last sequence update: | April 13, 2016 | |
Last modified: | January 19, 2022 | |
This is version 33 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |