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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Paracoccus sp. MKU1
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase complex NapAB. Receives electrons from NapB and catalyzes the reduction of nitrate to nitrite.UniRule annotationSAAS annotation

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Catalytic activityi

2 ferrocytochrome + nitrate + 2 H+ = 2 ferricytochrome + nitrite.UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi48Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi51Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi55Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi83Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei85Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei152Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei177Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei181Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei375Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei379Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei485Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei534Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei561Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei797Substrate; via amide nitrogenUniRule annotation1
Binding sitei805Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei822Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductaseUniRule annotationSAAS annotation
Biological processElectron transportUniRule annotationSAAS annotation, Nitrate assimilationUniRule annotationSAAS annotation, Transport
Ligand4Fe-4SUniRule annotationSAAS annotation, Iron, Iron-sulfur, Metal-binding, MolybdenumUniRule annotationSAAS annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.9.6.1UniRule annotation)
Gene namesi
Name:napAUniRule annotation
ORF Names:AQY21_26650Imported
OrganismiParacoccus sp. MKU1Imported
Taxonomic identifieri1745182 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeParacoccus
Proteomesi
  • UP000054373 Componenti: Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

PeriplasmUniRule annotation

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Sequence analysisAdd BLAST29
ChainiPRO_500886137130 – 831Periplasmic nitrate reductaseSequence analysisAdd BLAST802

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Component of the periplasmic nitrate reductase NapAB complex composed of NapA and NapB.UniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini41 – 974Fe-4S Mo/W bis-MGD-typeInterPro annotationAdd BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni214 – 221Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation8
Regioni245 – 249Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation5
Regioni264 – 266Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation3
Regioni511 – 512Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation2
Regioni721 – 730Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation10

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.SAAS annotation

Keywords - Domaini

SignalUniRule annotation

Family and domain databases

HAMAPiMF_01630 Nitrate_reduct_NapA, 1 hit
InterProiView protein in InterPro
IPR009010 Asp_de-COase-like_dom_sf
IPR006657 MoPterin_dinucl-bd_dom
IPR006656 Mopterin_OxRdtase
IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
IPR027467 MopterinOxRdtase_cofactor_BS
IPR010051 Periplasm_NO3_reductase_lsu
IPR006311 TAT_signal
IPR019546 TAT_signal_bac_arc
PANTHERiPTHR11615:SF123 PTHR11615:SF123, 1 hit
PfamiView protein in Pfam
PF04879 Molybdop_Fe4S4, 1 hit
PF00384 Molybdopterin, 1 hit
PF01568 Molydop_binding, 1 hit
SMARTiView protein in SMART
SM00926 Molybdop_Fe4S4, 1 hit
SUPFAMiSSF50692 SSF50692, 1 hit
TIGRFAMsiTIGR01706 NAPA, 1 hit
TIGR01409 TAT_signal_seq, 1 hit
PROSITEiView protein in PROSITE
PS51669 4FE4S_MOW_BIS_MGD, 1 hit
PS00551 MOLYBDOPTERIN_PROK_1, 1 hit
PS51318 TAT, 1 hit

Sequencei

Sequence statusi: Complete.

A0A0V0PTJ9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTISRRDLLK AQAAGIAAMA ANIPLSSQAQ PVPGGVESLQ ITWSKAPCRF
60 70 80 90 100
CGTGCGVMVG VKEGRVVATH GDLLAEVNRG LNCVKGYFLS KIMYGQDRLT
110 120 130 140 150
QPLLRKRDGI YAKDGEFTPV SWEEAFDTMA AQAKRVLKDK GPTAVGMFGS
160 170 180 190 200
GQWTIFEGYA ATKLMRAGFR SNNLDPNARH CMASAAYAFM RTFGMDEPMG
210 220 230 240 250
CYDDFEAADA FVLWGSNMAE MHPILWTRVA DRRLGHPHVK VAVLSTFTHR
260 270 280 290 300
SSDLADIPIV FKPGTDLAIL NYIANHIIQT GRVNRDFVDR HTTFVAGATG
310 320 330 340 350
IGYGLRDDDP REVAARTAED PAATTPSTFE EFAELVSEYT LEKVSELSGV
360 370 380 390 400
EPGFLEQLAE LYADPDRKVM SLWTMGFNQH VRGVWANQMV YNLHLLTGKI
410 420 430 440 450
SEPGNSPFSL TGQPSACGTA REVGTFAHRL PADMTVTNPE HRKHAEEIWR
460 470 480 490 500
IPEGLIPDKP GLHAVAQDRA LHDGTLNFYW IQVNNNLQAS PNNSNEAWPG
510 520 530 540 550
YRNPENFIVV SDAYPTVTAL AADLILPAAM WVEKEGAYGN AERRTHVWHQ
560 570 580 590 600
LVEAPGEARS DLWQMMEFSK RFTTDEVWPE EILAANPNYR GQSLFDVLFR
610 620 630 640 650
NGSVDRFDIS ELNPDYANQE AKHFGFYVQK GLFEEYAAFG RGHGHDLAPY
660 670 680 690 700
DTYHEVRGLR WPVVDGKETL WRYREGLDPY VEPGAGVQFY GNPDGKAKII
710 720 730 740 750
AVPYEPPAEP PDEEYNIWLV TGRVLEHWHS GSMTMRVPEL YRAFPGARCF
760 770 780 790 800
MNPEDARDMG FNQGAEVRIV SRRGEIRSRV ETRGRNRMPR GVIFVPWFDA
810 820 830
SQLINKVTLD ATDPISKQTD FKKCAVKLLS V
Length:831
Mass (Da):92,827
Last modified:March 16, 2016 - v1
Checksum:iECD0DAB495C8895F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
LLWQ01000225 Genomic DNA Translation: KRW93079.1
RefSeqiWP_058100605.1, NZ_LLWQ01000225.1

Genome annotation databases

EnsemblBacteriaiKRW93079; KRW93079; AQY21_26650

Similar proteinsi

Entry informationi

Entry nameiA0A0V0PTJ9_9RHOB
AccessioniPrimary (citable) accession number: A0A0V0PTJ9
Entry historyiIntegrated into UniProtKB/TrEMBL: March 16, 2016
Last sequence update: March 16, 2016
Last modified: May 23, 2018
This is version 20 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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