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Protein

Microsomal triglyceride transfer protein large subunit

Gene

mttp

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the transport of triglyceride, cholesteryl ester, and phospholipid between phospholipid surfaces. Required for the secretion of plasma lipoproteins that contain apolipoprotein B.By similarity

Activity regulationi

Inhibited by naringenin.1 Publication

GO - Molecular functioni

  • lipid binding Source: UniProtKB-KW
  • lipid transporter activity Source: ZFIN
  • phospholipid transporter activity Source: GO_Central

GO - Biological processi

Keywordsi

Biological processLipid transport, Transport
LigandLipid-binding

Enzyme and pathway databases

ReactomeiR-DRE-8866423 VLDL assembly
R-DRE-8963888 Chylomicron assembly

Names & Taxonomyi

Protein namesi
Recommended name:
Microsomal triglyceride transfer protein large subunit1 Publication
Gene namesi
Name:mttpCurated
Synonyms:mtpImported
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 1

Organism-specific databases

ZFINiZDB-GENE-040419-2 mttp

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Disruption phenotypei

Larvae do not absorb dietary neutral lipids, consume very little yolk, are small and die by 6 days post-fertilization (dpf) with pronounced edema. No defects in intestinal absorption of short chain fatty acids. Loss of visualization of neutral lipids in the vasculature, heart and head structures by oil red o staining. Levels of apolipoprotein B, vitellogenin and lipovitellin unchanged. No cardiovascular defects or global intestinal function impairment. Increased expression of Foza2 and Pgc1 proteins (PubMed:17176039). Leads to excess angiogenesis and yolk absorption defects. Decreased mRNA levels of flt1 at 24 hours post-fertilization (hpf) with no change in the mRNA levels of kdrl or flt4 or in other vascular genes (PubMed:22581286).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_500645176322 – 884Microsomal triglyceride transfer protein large subunitSequence analysisAdd BLAST863

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi172 ↔ 192PROSITE-ProRule annotation
Glycosylationi348N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Disulfide bondi438 ↔ 443PROSITE-ProRule annotation
Glycosylationi787N-linked (GlcNAc...) asparagine1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiA0A0R4IVV0
PRIDEiA0A0R4IVV0

Expressioni

Tissue specificityi

Highest expression in the proximal part of the anterior intestine. Lower expression in the distal part of the anterior intestine, in the posterior portion of the intestinal tube and liver. Very low expression levels in heart, brain, ovary, testis and kidney.1 Publication

Developmental stagei

Detected in the blastoderm margin by 4 hours post-fertilization (hpf). Expressed in the yolk syncytial layer (YSL) from 9 hpf to 24 hpf. By 48 hpf expression decreases in the extraembryonic YSL and is detected in the embryonic liver primordium and intestinal tube in which strongly expressed by 4 days post-fertilization (dpf). At 6 and 15 dpf expression is restricted to the two main liver lobes and the anterior part of the intestine including the intestinal bulb, but not detected in the pharynx or posterior intestine. Expressed in the enterocytes of the anterior part of intestine as well as in the hepatic cells in 15 dpf larvae. The total level of expression is very small before 2 hpf, increasing significantly between 2 and 5 hpf, and remaining high at 6 and 9 hpf. A significant decrease in the expression level is detected at 12 hpf and low level remains until the end of embryogenesis, which occurs by 72 hpf.1 Publication

Inductioni

Expression is strongly up-regulated in the anterior intestine and to a lesser extent in liver in response to feeding in both larvae and adults (at protein level) (PubMed:15614773). Protein levels are not changed in 1 or 6 hours after feeding (PubMed:17176039).2 Publications

Gene expression databases

ExpressionAtlasiA0A0R4IVV0 baseline and differential

Interactioni

Subunit structurei

Heterodimer; heterodimerizes with the protein disulfide isomerase. Interacts with apolipoprotein B.By similarity

Protein-protein interaction databases

STRINGi7955.ENSDARP00000024667

Structurei

3D structure databases

SMRiA0A0R4IVV0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 660VitellogeninPROSITE-ProRule annotationAdd BLAST635

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4337 Eukaryota
ENOG410YKCW LUCA
HOGENOMiHOG000113688
HOVERGENiHBG006416
KOiK14463
OMAiEHWQSIR

Family and domain databases

Gene3Di1.25.10.20, 1 hit
2.30.230.10, 1 hit
InterProiView protein in InterPro
IPR015819 Lipid_transp_b-sht_shell
IPR001747 Lipid_transpt_N
IPR011030 Lipovitellin_superhlx_dom
IPR039988 MTTP
IPR015816 Vitellinogen_b-sht_N
PANTHERiPTHR13024 PTHR13024, 1 hit
PfamiView protein in Pfam
PF01347 Vitellogenin_N, 1 hit
SMARTiView protein in SMART
SM00638 LPD_N, 1 hit
SUPFAMiSSF48431 SSF48431, 1 hit
SSF56968 SSF56968, 1 hit
PROSITEiView protein in PROSITE
PS51211 VITELLOGENIN, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

A0A0R4IVV0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MMPVAGLLLC VTAVLCTSAL GAGPRLDNGK LYRYSYSTEV GLNRPTGSPG
60 70 80 90 100
GNVGFRISSD VDINLAWRNP EIQDEQLLQV KISNIQVESA GKHSRKNNIF
110 120 130 140 150
HGSSAESILG KVRLEALQRP FLVLWKMGKI RSLYAQKAEP ATVKNLKRGV
160 170 180 190 200
ASMLMMQLKS GKMSEADASG KCLVEYKVNK HQVIRTKHLE TCKSQETGFT
210 220 230 240 250
THSPVLGISG KCAAETVITL ENGIIKSADA KETHVLSINA RHKAATKVLS
260 270 280 290 300
RQSLTLKAIE AGPAEVAGKD VAGVVKALDD KFLSVGVIVE KTKPKCKGCP
310 320 330 340 350
NLMETWKAVR SQLEPNSLSK AEAPRSFLTL VHSLRKSSKS EILTVLQNCS
360 370 380 390 400
KTALPQLVDA VTSAQTPSSL SAILEFLDFS KKDGLILQER FLYACGFASH
410 420 430 440 450
PTESMLQSLL EVSQGKIGST EIKESVVIIM GALLRKLCLK GACDLPAVLK
460 470 480 490 500
VKELLLAGPD STQEESEVQM YLLALKNALL PEGIPVLAKY AESEVGAYST
510 520 530 540 550
IAITALQRYD PALITAEVKK ALNRIYHQNQ RIYEKNVRAA AADVIMSSNP
560 570 580 590 600
SYMEVKNLLL SIGHLPHEMN KYMLSKIQDV LRFQMPAYKL VRQVMKDMIS
610 620 630 640 650
HNYDRFSKTG SSSAYSGFMA ETVDVTCTYN LDILYSGSGV LRRSNMNIYG
660 670 680 690 700
QSNNALLHGL QVTIEAQGLE SLIAATPDEG EEELESFAGM SALLFDVQLR
710 720 730 740 750
PVTFFKGYSD LMSKMFSMSG DPINVVKGLI LLTDHSQVIP LQSGLRASAE
760 770 780 790 800
FQAGLSIDIS GGMEFSLWYR ESKTSVNNRG ALVIIGNMTV DTDFVSAGVE
810 820 830 840 850
VGFETEATLD FITTVQFSEY PFLVCMQMDK TTFPFRETVS KQEKLPTGQM
860 870 880
FSRKRSRDQV VPGSEFPLHQ ENSNMCKKVF EPAW
Length:884
Mass (Da):96,988
Last modified:January 20, 2016 - v1
Checksum:iF152B03F919EA752
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q502S3Q502S3_DANRE
Microsomal triglyceride transfer pr...
mttp mtp
195Annotation score:
F1QBU6F1QBU6_DANRE
Microsomal triglyceride transfer pr...
mttp mtp
155Annotation score:
F1QGT1F1QGT1_DANRE
Microsomal triglyceride transfer pr...
mttp mtp
106Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti37S → G in CAD21747 (PubMed:15614773).Curated1
Sequence conflicti50G → E in CAD21747 (PubMed:15614773).Curated1
Sequence conflicti50G → E in AAI35021 (Ref. 3) Curated1
Sequence conflicti81K → Q in CAD21747 (PubMed:15614773).Curated1
Sequence conflicti81K → Q in AAI35021 (Ref. 3) Curated1
Sequence conflicti447A → V in CAD21747 (PubMed:15614773).Curated1
Sequence conflicti488A → T in CAD21747 (PubMed:15614773).Curated1
Sequence conflicti672L → P in CAD21747 (PubMed:15614773).Curated1
Sequence conflicti718M → T in CAD21747 (PubMed:15614773).Curated1
Sequence conflicti850M → T in AAI35021 (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ428850 mRNA Translation: CAD21747.1
BX908747 Genomic DNA No translation available.
CABZ01049941 Genomic DNA No translation available.
CABZ01050432 Genomic DNA No translation available.
CU914777 Genomic DNA No translation available.
FO704847 Genomic DNA No translation available.
BC135020 mRNA Translation: AAI35021.1
RefSeqiNP_998135.1, NM_212970.1
UniGeneiDr.105092
Dr.75507

Genome annotation databases

EnsembliENSDART00000015251; ENSDARP00000024667; ENSDARG00000008637
GeneIDi406207
KEGGidre:406207

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ428850 mRNA Translation: CAD21747.1
BX908747 Genomic DNA No translation available.
CABZ01049941 Genomic DNA No translation available.
CABZ01050432 Genomic DNA No translation available.
CU914777 Genomic DNA No translation available.
FO704847 Genomic DNA No translation available.
BC135020 mRNA Translation: AAI35021.1
RefSeqiNP_998135.1, NM_212970.1
UniGeneiDr.105092
Dr.75507

3D structure databases

SMRiA0A0R4IVV0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000024667

Proteomic databases

PaxDbiA0A0R4IVV0
PRIDEiA0A0R4IVV0

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000015251; ENSDARP00000024667; ENSDARG00000008637
GeneIDi406207
KEGGidre:406207

Organism-specific databases

CTDi4547
ZFINiZDB-GENE-040419-2 mttp

Phylogenomic databases

eggNOGiKOG4337 Eukaryota
ENOG410YKCW LUCA
HOGENOMiHOG000113688
HOVERGENiHBG006416
KOiK14463
OMAiEHWQSIR

Enzyme and pathway databases

ReactomeiR-DRE-8866423 VLDL assembly
R-DRE-8963888 Chylomicron assembly

Miscellaneous databases

PROiPR:A0A0R4IVV0

Gene expression databases

ExpressionAtlasiA0A0R4IVV0 baseline and differential

Family and domain databases

Gene3Di1.25.10.20, 1 hit
2.30.230.10, 1 hit
InterProiView protein in InterPro
IPR015819 Lipid_transp_b-sht_shell
IPR001747 Lipid_transpt_N
IPR011030 Lipovitellin_superhlx_dom
IPR039988 MTTP
IPR015816 Vitellinogen_b-sht_N
PANTHERiPTHR13024 PTHR13024, 1 hit
PfamiView protein in Pfam
PF01347 Vitellogenin_N, 1 hit
SMARTiView protein in SMART
SM00638 LPD_N, 1 hit
SUPFAMiSSF48431 SSF48431, 1 hit
SSF56968 SSF56968, 1 hit
PROSITEiView protein in PROSITE
PS51211 VITELLOGENIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMTP_DANRE
AccessioniPrimary (citable) accession number: A0A0R4IVV0
Secondary accession number(s): A4IGB9, Q8AXV7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2017
Last sequence update: January 20, 2016
Last modified: November 7, 2018
This is version 22 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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