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Protein

Thymidylate synthase

Gene

thyA

Organism
Streptococcus suis
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotationSAAS annotation

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei154PROSITE-ProRule annotation1
Active sitei154NucleophileUniRule annotation1
Binding sitei1815,10-methylenetetrahydrofolateUniRule annotation1
Binding sitei189dUMPUniRule annotation1
Binding sitei2785,10-methylenetetrahydrofolate; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi133 – 134dUMP; shared with dimeric partnerUniRule annotation2
Nucleotide bindingi178 – 181dUMPUniRule annotation4
Nucleotide bindingi219 – 221dUMPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferaseUniRule annotationSAAS annotationImported, Transferase
Biological processNucleotide biosynthesisUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00575

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotationSAAS annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotationImported
ORF Names:ERS132406_00013Imported, ERS132441_00069Imported, XK27_08945Imported
OrganismiStreptococcus suisImported
Taxonomic identifieri1307 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
Proteomesi
  • UP000075193 Componenti: Unassembled WGS sequence
  • UP000037847 Componenti: Unassembled WGS sequence
  • UP000072083 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 279Thymidylat_syntInterPro annotationAdd BLAST272

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Family and domain databases

CDDicd00351 TS_Pyrimidine_HMase, 1 hit
Gene3Di3.30.572.10, 1 hit
HAMAPiMF_00008 Thymidy_synth_bact, 1 hit
InterProiView protein in InterPro
IPR023451 Thymidate_synth/dCMP_Mease
IPR036926 Thymidate_synth/dCMP_Mease_sf
IPR000398 Thymidylate_synthase
IPR020940 Thymidylate_synthase_AS
PfamiView protein in Pfam
PF00303 Thymidylat_synt, 1 hit
PRINTSiPR00108 THYMDSNTHASE
SUPFAMiSSF55831 SSF55831, 1 hit
PROSITEiView protein in PROSITE
PS00091 THYMIDYLATE_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

A0A0M9FG31-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKADIIFKE NIRKIMEEGV FSENARPRYK DGNVANSKYV TGAFAEYDLS
60 70 80 90 100
KGEFPITTLR PIPIKSAIKE ILWIYQDQTN DLSVLQEKYG VQYWNDWEVN
110 120 130 140 150
GTGTIGERYG AIVKKHDIIS KILKQLEENP WNRRNVISLW DYEAFDQSAG
160 170 180 190 200
LLPCAFQTMF DVRRVDGEIY LDATLTQRSN DMLVAHHINA MQYVALQMMI
210 220 230 240 250
AKHFGWKVGK FFYFINNLHI YDNQFEQAEE LLRRTPSEVE PRLVLNVPDG
260 270
TNFFDIRAED FELVDYNPVK PQLKFDLAI
Length:279
Mass (Da):32,537
Last modified:December 9, 2015 - v1
Checksum:i8F623FE55D167E3C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FIGZ01000001 Genomic DNA Translation: CYU51145.1
FIIC01000001 Genomic DNA Translation: CYV37319.1
LDOL01000070 Genomic DNA Translation: KPA65251.1
RefSeqiWP_014637954.1, NZ_POQR01000020.1

Genome annotation databases

EnsemblBacteriaiCYU51145; CYU51145; ERS132406_00013
CYV37319; CYV37319; ERS132441_00069
KPA65251; KPA65251; XK27_08945
PATRICifig|1307.473.peg.2268

Similar proteinsi

Entry informationi

Entry nameiA0A0M9FG31_STRSU
AccessioniPrimary (citable) accession number: A0A0M9FG31
Entry historyiIntegrated into UniProtKB/TrEMBL: December 9, 2015
Last sequence update: December 9, 2015
Last modified: July 18, 2018
This is version 21 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

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