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Protein

Hydroxyacylglutathione hydrolase

Gene

gloB

Organism
Sphingopyxis sp. C-1
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid.UniRule annotationSAAS annotation

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Catalytic activityi

S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate.UniRule annotationSAAS annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn2+ ions per subunit.UniRule annotation

Pathwayi: methylglyoxal degradation

This protein is involved in step 2 of the subpathway that synthesizes (R)-lactate from methylglyoxal.UniRule annotationSAAS annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Hydroxyacylglutathione hydrolase (gloB)
This subpathway is part of the pathway methylglyoxal degradation, which is itself part of Secondary metabolite metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-lactate from methylglyoxal, the pathway methylglyoxal degradation and in Secondary metabolite metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi57Zinc 1UniRule annotation1
Metal bindingi59Zinc 1UniRule annotation1
Metal bindingi61Zinc 2UniRule annotation1
Metal bindingi62Zinc 2UniRule annotation1
Metal bindingi115Zinc 1UniRule annotation1
Metal bindingi134Zinc 1UniRule annotation1
Metal bindingi134Zinc 2UniRule annotation1
Metal bindingi172Zinc 2UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolaseUniRule annotationSAAS annotationImported
LigandMetal-bindingUniRule annotationSAAS annotation, ZincUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00619; UER00676

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyacylglutathione hydrolaseUniRule annotation (EC:3.1.2.6UniRule annotation)
Alternative name(s):
Glyoxalase IIUniRule annotation
Short name:
Glx IIUniRule annotation
Gene namesi
Name:gloBUniRule annotation
ORF Names:SC1_02837Imported
OrganismiSphingopyxis sp. C-1Imported
Taxonomic identifieri262667 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeSphingopyxis
Proteomesi
  • UP000037780 Componenti: Unassembled WGS sequence

Interactioni

Subunit structurei

Monomer.UniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 172Lactamase_BInterPro annotationAdd BLAST159

Sequence similaritiesi

Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.UniRule annotationSAAS annotation

Family and domain databases

CDDicd07723 hydroxyacylglutathione_hydrola, 1 hit
Gene3Di3.60.15.10, 1 hit
HAMAPiMF_01374 Glyoxalase_2, 1 hit
InterProiView protein in InterPro
IPR035680 Clx_II_MBL
IPR032282 HAGH_C
IPR017782 Hydroxyacylglutathione_Hdrlase
IPR001279 Metallo-B-lactamas
IPR036866 RibonucZ/Hydroxyglut_hydro
PfamiView protein in Pfam
PF16123 HAGH_C, 1 hit
PF00753 Lactamase_B, 1 hit
PIRSFiPIRSF005457 Glx, 1 hit
SMARTiView protein in SMART
SM00849 Lactamase_B, 1 hit
SUPFAMiSSF56281 SSF56281, 1 hit
TIGRFAMsiTIGR03413 GSH_gloB, 1 hit

Sequencei

Sequence statusi: Complete.

A0A0M8JI59-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAALDIVRIP VLSDNYVWLV HDPESGATMV VDPAVAEPVL AAAAERGWTI
60 70 80 90 100
TDIWNTHWHP DHTGGNAAIK EATGCTITGP AAEYARIPTL DVQVKGGDKV
110 120 130 140 150
RLGEHVADVW DVPAHTAGHI AYHFTHDAAI FVGDTMFAMG CGRLFEGTAE
160 170 180 190 200
QMFANMQKLG TLDDATRVYC AHEYTLSNAR FAVTVEPDNV ALAERLAAVE
210 220 230 240
SARAAGEATV PTSIGEERAT NPFLRAPSAA ELGRIRALKD AA
Length:242
Mass (Da):25,842
Last modified:December 9, 2015 - v1
Checksum:iE8112918AA5D8506
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BBRO01000001 Genomic DNA Translation: GAO79516.1
RefSeqiWP_062182741.1, NZ_BBRO01000001.1

Genome annotation databases

EnsemblBacteriaiGAO79516; GAO79516; SC1_02837

Similar proteinsi

Entry informationi

Entry nameiA0A0M8JI59_9SPHN
AccessioniPrimary (citable) accession number: A0A0M8JI59
Entry historyiIntegrated into UniProtKB/TrEMBL: December 9, 2015
Last sequence update: December 9, 2015
Last modified: February 28, 2018
This is version 16 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

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