UniProtKB - A0A0M1YSA3 (A0A0M1YSA3_9LEPT)
Protein
Protein-glutamate methylesterase/protein-glutamine glutaminase
Gene
cheB
Organism
Leptospira santarosai str. AIM
Status
Functioni
Involved in chemotaxis. Part of a chemotaxis signal transduction system that modulates chemotaxis in response to various stimuli. Catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins or MCP) by CheR. Also mediates the irreversible deamidation of specific glutamine residues to glutamic acid.UniRule annotation
Caution
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported
Catalytic activityi
- EC:3.5.1.44UniRule annotation
- [protein]-L-glutamate 5-O-methyl ester + H2O = H+ + L-glutamyl-[protein] + methanolUniRule annotationARBA annotationEC:3.1.1.61UniRule annotationARBA annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 167 | UniRule annotation | 1 | |
Active sitei | 193 | UniRule annotation | 1 | |
Active sitei | 289 | UniRule annotation | 1 |
GO - Molecular functioni
- phosphorelay response regulator activity Source: InterPro
- protein-glutamate methylesterase activity Source: UniProtKB-UniRule
- protein-glutamine glutaminase activity Source: UniProtKB-UniRule
GO - Biological processi
- chemotaxis Source: UniProtKB-UniRule
- protein deamination Source: UniProtKB-UniRule
- protein demethylation Source: UniProtKB-UniRule
Keywordsi
Molecular function | HydrolaseUniRule annotationARBA annotation |
Biological process | ChemotaxisUniRule annotation |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:cheBUniRule annotation ORF Names:LEP1GSC070_2318Imported |
Organismi | Leptospira santarosai str. AIMImported |
Taxonomic identifieri | 1193056 [NCBI] |
Taxonomic lineagei | Bacteria › Spirochaetes › Leptospirales › Leptospiraceae › Leptospira › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmUniRule annotationPTM / Processingi
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 54 | 4-aspartylphosphateUniRule annotation | 1 |
Post-translational modificationi
Phosphorylated by CheA. Phosphorylation of the N-terminal regulatory domain activates the methylesterase activity.UniRule annotation
Keywords - PTMi
PhosphoproteinUniRule annotationFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 3 – 120 | Response regulatoryInterPro annotationAdd BLAST | 118 | |
Domaini | 155 – 343 | CheB-type methylesteraseInterPro annotationAdd BLAST | 189 |
Domaini
Contains a C-terminal catalytic domain, and an N-terminal region which modulates catalytic activity.UniRule annotation
Sequence similaritiesi
Belongs to the CheB family.UniRule annotation
Family and domain databases
CDDi | cd16432, CheB_Rec, 1 hit cd00156, REC, 1 hit |
Gene3Di | 3.40.50.180, 1 hit |
HAMAPi | MF_00099, CheB_chemtxs, 1 hit |
InterProi | View protein in InterPro IPR008248, CheB-like IPR035909, CheB_C IPR011006, CheY-like_superfamily IPR000673, Sig_transdc_resp-reg_Me-estase IPR001789, Sig_transdc_resp-reg_receiver |
Pfami | View protein in Pfam PF01339, CheB_methylest, 1 hit PF00072, Response_reg, 1 hit |
PIRSFi | PIRSF000876, RR_chemtxs_CheB, 1 hit |
SMARTi | View protein in SMART SM00448, REC, 1 hit |
SUPFAMi | SSF52172, SSF52172, 1 hit SSF52738, SSF52738, 1 hit |
PROSITEi | View protein in PROSITE PS50122, CHEB, 1 hit PS50110, RESPONSE_REGULATORY, 1 hit |
i Sequence
Sequence statusi: Complete.
A0A0M1YSA3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIRVFIIDDS AVVRQVLAQI LNKDPEIEII GFASDPIFAS ERLSSVWPDV
60 70 80 90 100
FILDVEMPRM DGISFLKKIM SERPTPVILC STLAEKESET AVLAMKLGAI
110 120 130 140 150
DIIEKPKIGL KNFLEESEVL FTDSVKAASA ARIKFHQDGE SKENHRQTKA
160 170 180 190 200
DFSKISTTDK LIAIGTSTGG TQALEYILTQ LDVRCPGIVI VQHMPEKFTE
210 220 230 240 250
TFANRLNQIC EIQVKEAKDG DRIQLGSAYI APGNKHMEIY LSGAQFHVRV
260 270 280 290 300
LDGPLINRHR PSVDVLFNSV AKIAGKNAKG IIMTGMGNDG ANGLLKMRQT
310 320 330 340
GAFTIAQDET SCVVFGMPKE AILKGAVETI LPLSEIVREV QIF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AKWT02000052 Genomic DNA Translation: EMO83951.1 |
RefSeqi | WP_004460616.1, NZ_AKWT02000052.1 |
Genome annotation databases
EnsemblBacteriai | EMO83951; EMO83951; LEP1GSC070_2318 |
GeneIDi | 29741964 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AKWT02000052 Genomic DNA Translation: EMO83951.1 |
RefSeqi | WP_004460616.1, NZ_AKWT02000052.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Genome annotation databases
EnsemblBacteriai | EMO83951; EMO83951; LEP1GSC070_2318 |
GeneIDi | 29741964 |
Family and domain databases
CDDi | cd16432, CheB_Rec, 1 hit cd00156, REC, 1 hit |
Gene3Di | 3.40.50.180, 1 hit |
HAMAPi | MF_00099, CheB_chemtxs, 1 hit |
InterProi | View protein in InterPro IPR008248, CheB-like IPR035909, CheB_C IPR011006, CheY-like_superfamily IPR000673, Sig_transdc_resp-reg_Me-estase IPR001789, Sig_transdc_resp-reg_receiver |
Pfami | View protein in Pfam PF01339, CheB_methylest, 1 hit PF00072, Response_reg, 1 hit |
PIRSFi | PIRSF000876, RR_chemtxs_CheB, 1 hit |
SMARTi | View protein in SMART SM00448, REC, 1 hit |
SUPFAMi | SSF52172, SSF52172, 1 hit SSF52738, SSF52738, 1 hit |
PROSITEi | View protein in PROSITE PS50122, CHEB, 1 hit PS50110, RESPONSE_REGULATORY, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | A0A0M1YSA3_9LEPT | |
Accessioni | A0A0M1YSA3Primary (citable) accession number: A0A0M1YSA3 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | November 11, 2015 |
Last sequence update: | November 11, 2015 | |
Last modified: | August 12, 2020 | |
This is version 24 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |