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Entry version 31 (12 Aug 2020)
Sequence version 1 (11 Nov 2015)
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Protein

DNA topoisomerase 1

Gene

TOP1

Organism
Candida auris (Yeast)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Protein inferred from homologyi <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at the specific target site 5'-[CT]CCTTp site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

  • ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.PROSITE-ProRule annotation EC:5.6.2.1

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei707O-(3'-phospho-DNA)-tyrosine intermediatePROSITE-ProRule annotation1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionDNA-binding, Isomerase, Topoisomerase

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
DNA topoisomerase 1Curated (EC:5.6.2.1PROSITE-ProRule annotation)
Alternative name(s):
DNA topoisomerase ICurated
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:TOP1
ORF Names:QG37_01781
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiCandida auris (Yeast)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri498019 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesMetschnikowiaceaeClavisporaClavispora/Candida clade
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000037122 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Unassembled WGS sequence

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004476281 – 749DNA topoisomerase 1Add BLAST749

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection describes interesting single amino acid sites on the sequence that are not defined in any other subsection. This subsection can be displayed in different sections ('Function', 'PTM / Processing', 'Pathology and Biotech') according to its content.<p><a href='/help/site' target='_top'>More...</a></p>Sitei277Interaction with DNABy similarity1
Sitei325Interaction with DNABy similarity1
Sitei356Interaction with DNABy similarity1
Sitei413Interaction with DNABy similarity1
Sitei439Interaction with DNABy similarity1
Sitei482Interaction with DNABy similarity1
Sitei539Interaction with DNABy similarity1
Sitei557Interaction with DNABy similarity1

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni338 – 339Interaction with DNABy similarity2
Regioni401 – 406Interaction with DNABy similarity6
Regioni493 – 495Interaction with DNABy similarity3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi37 – 90Lys-richPROSITE-ProRule annotationAdd BLAST54

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the type IB topoisomerase family.Curated

Family and domain databases

Conserved Domains Database

More...
CDDi
cd00659, Topo_IB_C, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
1.10.10.41, 1 hit
1.10.132.10, 1 hit
2.170.11.10, 1 hit
3.90.15.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR011010, DNA_brk_join_enz
IPR013034, DNA_topo_DNA_db_N_dom1
IPR013030, DNA_topo_DNA_db_N_dom2
IPR001631, TopoI
IPR018521, TopoI_AS
IPR025834, TopoI_C_dom
IPR014711, TopoI_cat_a-hlx-sub_euk
IPR014727, TopoI_cat_a/b-sub_euk
IPR013500, TopoI_cat_euk
IPR008336, TopoI_DNA-bd_euk
IPR036202, TopoI_DNA-bd_euk_N_sf
IPR013499, TopoI_euk

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF14370, Topo_C_assoc, 1 hit
PF01028, Topoisom_I, 1 hit
PF02919, Topoisom_I_N, 1 hit

Protein Motif fingerprint database; a protein domain database

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PRINTSi
PR00416, EUTPISMRASEI

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00435, TOPEUc, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF56349, SSF56349, 1 hit
SSF56741, SSF56741, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00176, TOPOISOMERASE_I_EUK, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

A0A0L0P4F8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSDSEDVALS ELSSRNGIKK EDEEMPLAQL NGNGNAKLRK RKHSEKSKDD
60 70 80 90 100
HKDKKRKKEK KLSKEKVNNK VKDELISAPV TPKKTPKISK TPVSATSSPA
110 120 130 140 150
PKKEDSVETD EGYKWWEDEN FGEGDERWQT LEHNGVLFPP PYEPLPSYVK
160 170 180 190 200
LYYEGKPVDL PPLAEEVAGY FAAMIETDHA KNPVFQQNFF NDFLQVLKEG
210 220 230 240 250
GGCNVDIKEF SKCDFSKMAA FFEKQREEKK SMSKAEKQRI KEEKEKLEEP
260 270 280 290 300
FKTCLMDGRR ENVGNFRVEP PGLFRGRGAH PKTGKFKRRV FPEDITLNLG
310 320 330 340 350
EGAPIPPPPE GHQWGGIRHD KTVVWLAMWR ENISDTFKYV KLAANSSIKG
360 370 380 390 400
ISDMKKFETA RKLKDHIERI RADYQKMLKD QFMQNRQIAT ATYLIDIFAL
410 420 430 440 450
RAGGEKGEDE ADTVGCCSLR YEHVTLKPPN KVIFDFLGKD SIRFYQEVEV
460 470 480 490 500
DRQVFKNLRI FKKEPKGPGD DLFDRITPTI LNKHLQNYMK GLTAKVFRTY
510 520 530 540 550
NASKTMQDQM DLISNEGTVA EKVVKYNAAN RTVAILCNHQ RTVSKNHEQA
560 570 580 590 600
VKRINDKIRE LEWQKIRLKK MILDIDETEK KDNPKYFEEL DDLTNDEMNV
610 620 630 640 650
IVERVIERQK DQAEKKWNRD NEKRKMEKEE LLTKADLQEK LDKIEETRKD
660 670 680 690 700
YLEEIKTGVV RATKGSSVSS LKSKVETVEN RIVNTSYQLK DKEDNSEVSL
710 720 730 740
GTSKMNYIDP RLTVVFAKKF NVPIEKLFTK TLRDKFNWAI ESTDENWRF
Length:749
Mass (Da):86,838
Last modified:November 11, 2015 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iC5D65A3332875F72
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
LGST01000016 Genomic DNA Translation: KNE00911.1

NCBI Reference Sequences

More...
RefSeqi
XP_018170634.1, XM_018311275.1

Genome annotation databases

Ensembl fungal genome annotation project

More...
EnsemblFungii
KNE00911; KNE00911; QG37_01781

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
LGST01000016 Genomic DNA Translation: KNE00911.1
RefSeqiXP_018170634.1, XM_018311275.1

3D structure databases

Database of comparative protein structure models

More...
ModBasei
Search...

SWISS-MODEL Interactive Workspace

More...
SWISS-MODEL-Workspacei
Submit a new modelling project...

Genome annotation databases

EnsemblFungiiKNE00911; KNE00911; QG37_01781

Family and domain databases

CDDicd00659, Topo_IB_C, 1 hit
Gene3Di1.10.10.41, 1 hit
1.10.132.10, 1 hit
2.170.11.10, 1 hit
3.90.15.10, 1 hit
InterProiView protein in InterPro
IPR011010, DNA_brk_join_enz
IPR013034, DNA_topo_DNA_db_N_dom1
IPR013030, DNA_topo_DNA_db_N_dom2
IPR001631, TopoI
IPR018521, TopoI_AS
IPR025834, TopoI_C_dom
IPR014711, TopoI_cat_a-hlx-sub_euk
IPR014727, TopoI_cat_a/b-sub_euk
IPR013500, TopoI_cat_euk
IPR008336, TopoI_DNA-bd_euk
IPR036202, TopoI_DNA-bd_euk_N_sf
IPR013499, TopoI_euk
PfamiView protein in Pfam
PF14370, Topo_C_assoc, 1 hit
PF01028, Topoisom_I, 1 hit
PF02919, Topoisom_I_N, 1 hit
PRINTSiPR00416, EUTPISMRASEI
SMARTiView protein in SMART
SM00435, TOPEUc, 1 hit
SUPFAMiSSF56349, SSF56349, 1 hit
SSF56741, SSF56741, 1 hit
PROSITEiView protein in PROSITE
PS00176, TOPOISOMERASE_I_EUK, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiTOP1_CANAR
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: A0A0L0P4F8
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 3, 2019
Last sequence update: November 11, 2015
Last modified: August 12, 2020
This is version 31 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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