UniProtKB - A0A0K8P8E7 (MHETH_IDESA)
Protein
Mono(2-hydroxyethyl) terephthalate hydrolase
Gene
ISF6_0224
Organism
Ideonella sakaiensis (strain NBRC 110686 / TISTR 2288 / 201-F6)
Status
Functioni
Involved in the degradation and assimilation of the plastic poly(ethylene terephthalate) (PET), which allows I.sakaiensis to use PET as its major energy and carbon source for growth. Likely acts synergistically with PETase to depolymerize PET. Catalyzes the hydrolysis of mono(2-hydroxyethyl) terephthalate (MHET) into its two environmentally benign monomers, terephthalate and ethylene glycol. Does not show activity against PET, bis(hydroxyethyl) terephthalate (BHET), pNP-aliphatic esters or typical aromatic ester compounds catalyzed by the tannase family enzymes, such as ethyl gallate and ethyl ferulate.1 Publication
Miscellaneous
The calcium ion is located far from the active site and appears to have a role in stabilization of the lid domain.By similarity
Catalytic activityi
Kineticsi
kcat is 31 sec(-1) for the hydrolysis of mono(2-hydroxyethyl) terephthalate (at pH 7 and 30 degrees Celsius).1 Publication
- KM=7.3 µM for mono(2-hydroxyethyl) terephthalate (at pH 7 and 30 degrees Celsius)1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 132 | 4-[(2-hydroxyethoxy)carbonyl]benzoate; via amide nitrogen1 Publication | 1 | |
Active sitei | 225 | Acyl-ester intermediate1 Publication | 1 | |
Binding sitei | 226 | 4-[(2-hydroxyethoxy)carbonyl]benzoate; via amide nitrogen1 Publication | 1 | |
Metal bindingi | 304 | Calcium1 Publication | 1 | |
Metal bindingi | 307 | Calcium1 Publication | 1 | |
Metal bindingi | 309 | Calcium; via carbonyl oxygen1 Publication | 1 | |
Metal bindingi | 311 | Calcium1 Publication | 1 | |
Metal bindingi | 313 | Calcium; via carbonyl oxygen1 Publication | 1 | |
Binding sitei | 411 | 4-[(2-hydroxyethoxy)carbonyl]benzoate1 Publication | 1 | |
Binding sitei | 416 | 4-[(2-hydroxyethoxy)carbonyl]benzoate1 Publication | 1 | |
Active sitei | 492 | Charge relay system1 Publication | 1 | |
Active sitei | 528 | Charge relay system1 Publication | 1 | |
Binding sitei | 528 | 4-[(2-hydroxyethoxy)carbonyl]benzoate1 Publication | 1 |
GO - Molecular functioni
- carboxylic ester hydrolase activity Source: UniProtKB
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- cellular response to organic substance Source: UniProtKB
- xenobiotic catabolic process Source: UniProtKB
Keywordsi
Molecular function | Hydrolase, Serine esterase |
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
BioCyci | MetaCyc:MONOMER-19899 |
Protein family/group databases
ESTHERi | idesa-mheth, Tannase |
Names & Taxonomyi
Protein namesi | Recommended name: Mono(2-hydroxyethyl) terephthalate hydrolase1 Publication (EC:3.1.1.1021 Publication)Short name: MHET hydrolase1 Publication Short name: MHETase1 Publication |
Gene namesi | ORF Names:ISF6_0224Imported |
Organismi | Ideonella sakaiensis (strain NBRC 110686 / TISTR 2288 / 201-F6) |
Taxonomic identifieri | 1547922 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Ideonella |
Proteomesi |
|
Subcellular locationi
- Cell outer membrane 1 Publication; Lipid-anchor PROSITE-ProRule annotation1 Publication
GO - Cellular componenti
- cell outer membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell outer membrane, MembranePathology & Biotechi
Biotechnological usei
Has potential for application in environmental remediation and biological recycling of PET waste products.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 225 | S → A: Loss of catalytic activity towards MHET. 1 Publication | 1 | |
Mutagenesisi | 411 | R → A or Q: Almost complete loss of catalytic activity towards MHET. 1 Publication | 1 | |
Mutagenesisi | 416 | S → A: Gains a low activity towards BHET (bis-(2-hydroxyethyl) terephthalate); when associated with N-424. 1 Publication | 1 | |
Mutagenesisi | 424 | F → N: Gains a low activity towards BHET (bis-(2-hydroxyethyl) terephthalate); when associated with A-416. 1 Publication | 1 | |
Mutagenesisi | 492 | D → A: Loss of catalytic activity towards MHET. 1 Publication | 1 | |
Mutagenesisi | 528 | H → A: Loss of catalytic activity towards MHET. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 17 | PROSITE-ProRule annotationAdd BLAST | 17 | |
ChainiPRO_5005513859 | 18 – 603 | Mono(2-hydroxyethyl) terephthalate hydrolaseAdd BLAST | 586 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 18 | N-palmitoyl cysteinePROSITE-ProRule annotation | 1 | |
Lipidationi | 18 | S-diacylglycerol cysteinePROSITE-ProRule annotation | 1 | |
Disulfide bondi | 51 ↔ 92 | 1 Publication | ||
Disulfide bondi | 224 ↔ 529 | 1 Publication | ||
Disulfide bondi | 303 ↔ 320 | 1 Publication | ||
Disulfide bondi | 340 ↔ 348 | 1 Publication | ||
Disulfide bondi | 577 ↔ 599 | 1 Publication |
Keywords - PTMi
Disulfide bond, Lipoprotein, PalmitateExpressioni
Inductioni
Highly up-regulated during growth on PET film.1 Publication
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | A0A0K8P8E7 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 25 – 44 | Pro-richPROSITE-ProRule annotationAdd BLAST | 20 |
Sequence similaritiesi
Belongs to the tannase family.Curated
Keywords - Domaini
SignalFamily and domain databases
InterProi | View protein in InterPro IPR029058, AB_hydrolase IPR011118, Tannase/feruloyl_esterase |
PANTHERi | PTHR33938, PTHR33938, 1 hit |
Pfami | View protein in Pfam PF07519, Tannase, 1 hit |
SUPFAMi | SSF53474, SSF53474, 2 hits |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
A0A0K8P8E7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQTTVTTMLL ASVALAACAG GGSTPLPLPQ QQPPQQEPPP PPVPLASRAA
60 70 80 90 100
CEALKDGNGD MVWPNAATVV EVAAWRDAAP ATASAAALPE HCEVSGAIAK
110 120 130 140 150
RTGIDGYPYE IKFRLRMPAE WNGRFFMEGG SGTNGSLSAA TGSIGGGQIA
160 170 180 190 200
SALSRNFATI ATDGGHDNAV NDNPDALGTV AFGLDPQARL DMGYNSYDQV
210 220 230 240 250
TQAGKAAVAR FYGRAADKSY FIGCSEGGRE GMMLSQRFPS HYDGIVAGAP
260 270 280 290 300
GYQLPKAGIS GAWTTQSLAP AAVGLDAQGV PLINKSFSDA DLHLLSQAIL
310 320 330 340 350
GTCDALDGLA DGIVDNYRAC QAAFDPATAA NPANGQALQC VGAKTADCLS
360 370 380 390 400
PVQVTAIKRA MAGPVNSAGT PLYNRWAWDA GMSGLSGTTY NQGWRSWWLG
410 420 430 440 450
SFNSSANNAQ RVSGFSARSW LVDFATPPEP MPMTQVAARM MKFDFDIDPL
460 470 480 490 500
KIWATSGQFT QSSMDWHGAT STDLAAFRDR GGKMILYHGM SDAAFSALDT
510 520 530 540 550
ADYYERLGAA MPGAAGFARL FLVPGMNHCS GGPGTDRFDM LTPLVAWVER
560 570 580 590 600
GEAPDQISAW SGTPGYFGVA ARTRPLCPYP QIARYKGSGD INTEANFACA
APP
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BBYR01000104 Genomic DNA Translation: GAP38911.1 |
RefSeqi | WP_054022745.1, NZ_BBYR01000104.1 |
Genome annotation databases
EnsemblBacteriai | GAP38911; GAP38911; ISF6_0224 |
KEGGi | ag:GAP38911 |
Similar proteinsi
Cross-referencesi
Web resourcesi
Protein Spotlight Of plastic and men - Issue 181 of July 2016 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BBYR01000104 Genomic DNA Translation: GAP38911.1 |
RefSeqi | WP_054022745.1, NZ_BBYR01000104.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6JTT | X-ray | 2.51 | A/B/C | 17-603 | [»] | |
6JTU | X-ray | 2.10 | A/B/C | 17-603 | [»] | |
6QG9 | X-ray | 2.05 | A/B/C/D/E/F/G/H/I/J | 20-603 | [»] | |
6QGA | X-ray | 2.10 | A/B/C/D/E/F | 20-603 | [»] | |
6QGB | X-ray | 2.20 | A/B/C/D/E/F | 20-603 | [»] | |
6QZ1 | X-ray | 1.70 | A | 40-603 | [»] | |
6QZ2 | X-ray | 1.90 | A/B/C/D/E/F/G/H/I/J | 1-603 | [»] | |
6QZ3 | X-ray | 1.60 | A | 1-603 | [»] | |
6QZ4 | X-ray | 1.80 | A/B | 1-603 | [»] | |
SMRi | A0A0K8P8E7 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein family/group databases
ESTHERi | idesa-mheth, Tannase |
Genome annotation databases
EnsemblBacteriai | GAP38911; GAP38911; ISF6_0224 |
KEGGi | ag:GAP38911 |
Enzyme and pathway databases
BioCyci | MetaCyc:MONOMER-19899 |
Family and domain databases
InterProi | View protein in InterPro IPR029058, AB_hydrolase IPR011118, Tannase/feruloyl_esterase |
PANTHERi | PTHR33938, PTHR33938, 1 hit |
Pfami | View protein in Pfam PF07519, Tannase, 1 hit |
SUPFAMi | SSF53474, SSF53474, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MHETH_IDESA | |
Accessioni | A0A0K8P8E7Primary (citable) accession number: A0A0K8P8E7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 11, 2016 |
Last sequence update: | November 11, 2015 | |
Last modified: | February 10, 2021 | |
This is version 27 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Protein Spotlight
Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries