Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Gene

ispE

Organism
Mycobacterium goodii
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol.UniRule annotationSAAS annotation

Catalytic activityi

ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol.UniRule annotationSAAS annotation

Pathwayi: isopentenyl diphosphate biosynthesis via DXP pathway

This protein is involved in step 3 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr), 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr)
  2. 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD), 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD)
  3. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE), 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE)
  4. 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF), 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF)
  5. 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG), 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG), 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG)
  6. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH), 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH), 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)
This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via DXP pathway, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei13UniRule annotation1
Active sitei140UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi98 – 108ATPUniRule annotationAdd BLAST11

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinaseUniRule annotationSAAS annotationImported, Transferase
Biological processIsoprene biosynthesisUniRule annotation
LigandATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00056; UER00094

Names & Taxonomyi

Protein namesi
Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinaseUniRule annotation (EC:2.7.1.148UniRule annotation)
Short name:
CMKUniRule annotation
Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinaseUniRule annotation
Gene namesi
Name:ispEUniRule annotation
ORF Names:AFA91_13525Imported
OrganismiMycobacterium goodiiImported
Taxonomic identifieri134601 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycolicibacterium
Proteomesi
  • UP000062255 Componenti: Chromosome

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini91 – 148GHMP_kinases_NInterPro annotationAdd BLAST58
Domaini209 – 274GHMP_kinases_CInterPro annotationAdd BLAST66

Sequence similaritiesi

Belongs to the GHMP kinase family. IspE subfamily.UniRule annotationSAAS annotation

Phylogenomic databases

KOiK00919

Family and domain databases

Gene3Di3.30.230.10, 1 hit
3.30.70.890, 1 hit
HAMAPiMF_00061 IspE, 1 hit
InterProiView protein in InterPro
IPR013750 GHMP_kinase_C_dom
IPR036554 GHMP_kinase_C_sf
IPR006204 GHMP_kinase_N_dom
IPR004424 IspE
IPR020568 Ribosomal_S5_D2-typ_fold
IPR014721 Ribosomal_S5_D2-typ_fold_subgr
PfamiView protein in Pfam
PF08544 GHMP_kinases_C, 1 hit
PF00288 GHMP_kinases_N, 1 hit
PIRSFiPIRSF010376 IspE, 1 hit
SUPFAMiSSF54211 SSF54211, 1 hit
SSF55060 SSF55060, 1 hit
TIGRFAMsiTIGR00154 ispE, 1 hit

Sequencei

Sequence statusi: Complete.

A0A0K0X5R8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTGSVTVRV PGKVNLYLSV GDVRDDGYHD LTTVFHAVSL LDEVTVRNAD
60 70 80 90 100
VLSLQMSGEG SETLPVDERN LAWRAAELMA EHVGRAPDVA ITIEKSIPVA
110 120 130 140 150
GGMAGGSADA AGVLVAMNTL WELGVPRRDL HALAAQLGSD VPFALHGGTA
160 170 180 190 200
LGSGRGEELA TVLARNTFHW VLAFAHRGLS TPKVFGELDR LRADATRGLP
210 220 230 240 250
RADEPEPVLA ALASGDPAEL AALLGNDLQP AALSLYPELR RTLRAGVEAG
260 270 280 290 300
ALAGMVSGSG PTCAFLCASA SAAVDVGIQL SGAGVCRTVR VASGPVHGAH

VVPAPSAS
Length:308
Mass (Da):31,582
Last modified:November 11, 2015 - v1
Checksum:i08E90C3E704D9823
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP012150 Genomic DNA Translation: AKS32731.1
RefSeqiWP_049745162.1, NZ_CP012150.1

Genome annotation databases

EnsemblBacteriaiAKS32731; AKS32731; AFA91_13525
KEGGimgo:AFA91_13525
PATRICifig|134601.6.peg.2806

Similar proteinsi

Entry informationi

Entry nameiA0A0K0X5R8_MYCGD
AccessioniPrimary (citable) accession number: A0A0K0X5R8
Entry historyiIntegrated into UniProtKB/TrEMBL: November 11, 2015
Last sequence update: November 11, 2015
Last modified: June 20, 2018
This is version 19 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health