UniProtKB - A0A0H3MAA9 (CPNT_MYCBP)
Protein
Outer membrane channel protein CpnT
Gene
cpnT
Organism
Mycobacterium bovis (strain BCG / Pasteur 1173P2)
Status
Functioni
The N-terminal domain (NTD) forms an outer membrane channel and is used for uptake of nutrients across the outer membrane. Also confers susceptibility to structurally different antibiotics and antituberculosis drugs, and to toxic immune factors such as nitric oxide (NO). The C-terminal domain (TNT) is dispensable for normal growth in macrophages.1 Publication
Catalytic activityi
- EC:3.2.2.5By similarity
Activity regulationi
Glycohydrolase activity is completely inhibited by interaction with the immunity factor for TNT (IFT) homolog. This inhibition protects M.bovis from self-poisoning.By similarity
GO - Molecular functioni
- NAD glycohydrolase activity Source: UniProtKB-EC
- porin activity Source: UniProtKB-KW
GO - Biological processi
- ion transport Source: UniProtKB-KW
Keywordsi
Molecular function | Hydrolase, Porin |
Biological process | Ion transport, Transport |
Ligand | NAD |
Enzyme and pathway databases
BioCyci | MBOV410289:G1G1S-4339-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Outer membrane channel protein CpnT1 PublicationCleaved into the following 2 chains: N-terminal channel domainCurated Alternative name(s): NAD(+) glycohydrolaseBy similarity (EC:3.2.2.5By similarity) |
Gene namesi | Name:cpnT1 Publication Ordered Locus Names:BCG_3960cImported |
Organismi | Mycobacterium bovis (strain BCG / Pasteur 1173P2) |
Taxonomic identifieri | 410289 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Subcellular locationi
Other locations
- Cell outer membrane By similarity
Extracellular region or secreted
- Secreted By similarity
Other locations
- Cell surface By similarity
Note: M.bovis does not permeabilize phagosomes and does not escape from phagosomes. Thus, the C-terminal TNT domain is likely trapped in phagosomes during M.bovis BCG infection, where it is incapable of inducing necrosis of macrophages.1 Publication
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Other locations
- cell outer membrane Source: UniProtKB-SubCell
- cell surface Source: UniProtKB-SubCell
- pore complex Source: UniProtKB-KW
Keywords - Cellular componenti
Cell outer membrane, Membrane, SecretedPathology & Biotechi
Disruption phenotypei
Deletion of the gene confers high-level drug resistance to a wide range of antibiotics and antituberculosis drugs, and protects M.bovis from killing in macrophages that prevent intracellular mycobacterial replication. Increases resistance to small, hydrophilic antibiotics, to antituberculosis drugs, to large, hydrophobic or hydrophilic antibiotics such as erythromycin, rifampicin and streptomycin, and to nitric oxide. Under in vivo conditions, such as in human THP-1 macrophages, absence of the gene drastically reduces intracellular growth of M.bovis.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000437785 | 1 – 846 | Outer membrane channel protein CpnTAdd BLAST | 846 | |
ChainiPRO_0000437786 | 1 – ? | N-terminal channel domainCurated | ||
ChainiPRO_0000437787 | ? – 846 | Tuberculosis necrotizing toxin homologCurated |
Post-translational modificationi
The C-terminal domain (TNT) is probably cleaved.By similarity
Interactioni
Subunit structurei
Interacts with the immunity factor for TNT (IFT) homolog.
By similarityFamily & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 443 | NTDBy similarityAdd BLAST | 443 | |
Regioni | 651 – 846 | TNTBy similarityAdd BLAST | 196 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 2 – 229 | Ala-richPROSITE-ProRule annotationAdd BLAST | 228 | |
Compositional biasi | 445 – 586 | Pro-richPROSITE-ProRule annotationAdd BLAST | 142 |
Keywords - Domaini
Transmembrane, Transmembrane beta strandPhylogenomic databases
HOGENOMi | CLU_013587_0_0_11 |
OMAi | RNGLCSI |
Family and domain databases
InterProi | View protein in InterPro IPR025331, TNT |
Pfami | View protein in Pfam PF14021, TNT, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
A0A0H3MAA9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAPLAVDPAA LDSAGGAVVA AGAGLGAVIS SLTAALAGCA GMAGDDPAGA
60 70 80 90 100
VFGRSYDGSA AALVQAMSVA RNGLCNLGDG VRMSAHNYSL AEAMSDVAGR
110 120 130 140 150
AAPLPAPPPS GCVGVGAPPS AVGGGGGAPK GWGWVAPYIG MIWPNGDSTK
160 170 180 190 200
LRAAAVAWRS AGTQFALTEI QSTAGPMGVI RAQQLPEAGL IESAFADAYA
210 220 230 240 250
STTAVVGQCH QLAAQLDAYA ARIDAVHAAV LDLLARICDP LTGIKEVWEF
260 270 280 290 300
LTDQDEDEIQ RIAHDIAVVV DQFSGEVDAL AAEITAVVSH AEAVITAMAD
310 320 330 340 350
HAGKQWDRFL HSNPVGVVID GTGQQLKGFG EEAFGMAKDS WDLGPLRASI
360 370 380 390 400
DPFGWYRSWE EMLTGMAPLA GLGGENAPGV VESWKQFGKS LIHWDEWTTN
410 420 430 440 450
PNEALGKTVF DAATLALPGG PLSKLGSKGR DILAGVRGLK ERLEPTTPHL
460 470 480 490 500
EPPATPPRPG PQPPRIEPPE SGHPAPAPAA KPAPVPANGP LPHSPTESKP
510 520 530 540 550
PPVDRPAEPV APSSASAGQP RVSAATTPGT HVPHGLPQPG EHVPAQAPPA
560 570 580 590 600
TTLLGGPPVE SAPATAHQPQ WATTPAAPAA APHSTPGGVH STESGPHGRS
610 620 630 640 650
LSAHGSEPTH DGASHGSGHG SGSEPPGLHA PHREQQLAMH SNEPAGEGWH
660 670 680 690 700
RLSDEAVDPQ YGEPLSRHWD FTDNPADRSR INPVVAQLME DPNAPFGRDP
710 720 730 740 750
QGQPYTQERY QERFNSVGPW GQQYSNFPPN NGAVPGTRIA YTNLEKFLSD
760 770 780 790 800
YGPQLDRIGG DQGKYLAIME HGRPASWEQR ALHVTSLRDP YHAYTIDWLP
810 820 830 840
EGWFIEVSEV APGCGQPGGS IQVRIFDHQN EMRKVEELIR RGVLRQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AM408590 Genomic DNA Translation: CAL73950.1 |
RefSeqi | WP_003899759.1, NC_008769.1 |
Genome annotation databases
KEGGi | mbb:BCG_3960c |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AM408590 Genomic DNA Translation: CAL73950.1 |
RefSeqi | WP_003899759.1, NC_008769.1 |
3D structure databases
SMRi | A0A0H3MAA9 |
ModBasei | Search... |
Genome annotation databases
KEGGi | mbb:BCG_3960c |
Phylogenomic databases
HOGENOMi | CLU_013587_0_0_11 |
OMAi | RNGLCSI |
Enzyme and pathway databases
BioCyci | MBOV410289:G1G1S-4339-MONOMER |
Family and domain databases
InterProi | View protein in InterPro IPR025331, TNT |
Pfami | View protein in Pfam PF14021, TNT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CPNT_MYCBP | |
Accessioni | A0A0H3MAA9Primary (citable) accession number: A0A0H3MAA9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 2, 2016 |
Last sequence update: | September 16, 2015 | |
Last modified: | April 7, 2021 | |
This is version 24 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |