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UniProtKB - A0A0H3H456 (DHAM_KLEOK)
Protein
PEP-dependent dihydroxyacetone kinase, phosphoryl donor subunit DhaM
Gene
dhaM
Organism
Klebsiella oxytoca (strain ATCC 8724 / DSM 4798 / JCM 20051 / NBRC 3318 / NRRL B-199 / KCTC 1686)
Status
Functioni
Component of the dihydroxyacetone kinase complex, which is responsible for the phosphoenolpyruvate (PEP)-dependent phosphorylation of dihydroxyacetone. DhaM serves as the phosphoryl donor. Is phosphorylated by phosphoenolpyruvate in an EI- and HPr-dependent reaction, and a phosphorelay system on histidine residues finally leads to phosphoryl transfer to DhaL and dihydroxyacetone.
By similarityMiscellaneous
Unlike the carbohydrate-specific transporters of the PTS, the complex DhaKLM has no transport activity.By similarity
Caution
Was reported to be a protein deacetylase that removes acetyl groups on specific lysine residues in target proteins (PubMed:26716769). However, later experiments demonstrate that the protein ortholog in E.coli does not have any protein deacetylase activity; the discrepancy observed seems to be due to contaminants having proteolytic activity (PubMed:29939131).2 Publications
Catalytic activityi
- EC:2.7.1.121By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 9 | Tele-phosphohistidine intermediatePROSITE-ProRule annotation | 1 | |
Active sitei | 170 | Pros-phosphohistidine intermediatePROSITE-ProRule annotation | 1 | |
Active sitei | 432 | Tele-phosphohistidine intermediateBy similarity | 1 |
GO - Molecular functioni
- phosphoenolpyruvate-glycerone phosphotransferase activity Source: UniProtKB-EC
GO - Biological processi
- glycerol catabolic process Source: InterPro
- phosphoenolpyruvate-dependent sugar phosphotransferase system Source: InterPro
Keywordsi
Molecular function | Transferase |
Names & Taxonomyi
Protein namesi | Recommended name: PEP-dependent dihydroxyacetone kinase, phosphoryl donor subunit DhaMBy similarity (EC:2.7.1.121By similarity)Alternative name(s): Dihydroxyacetone kinase subunit MBy similarity |
Gene namesi | Name:dhaMBy similarity Ordered Locus Names:KOX_03180Imported |
Organismi | Klebsiella oxytoca (strain ATCC 8724 / DSM 4798 / JCM 20051 / NBRC 3318 / NRRL B-199 / KCTC 1686) |
Taxonomic identifieri | 1006551 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Klebsiella › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000435889 | 1 – 472 | PEP-dependent dihydroxyacetone kinase, phosphoryl donor subunit DhaMAdd BLAST | 472 |
Interactioni
Subunit structurei
Homodimer. The dihydroxyacetone kinase complex is composed of a homodimer of DhaM, a homodimer of DhaK and the subunit DhaL.
By similarityFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 135 | PTS EIIA type-4PROSITE-ProRule annotationAdd BLAST | 135 | |
Domaini | 156 – 243 | HPrPROSITE-ProRule annotationAdd BLAST | 88 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 266 – 472 | PTS EI-like, N-terminal partBy similarityAdd BLAST | 207 |
Domaini
Consists of three domains. The N-terminal dimerization domain has the same fold as the IIA domain of the mannose transporter of the bacterial phosphoenolpyruvate:sugar phosphotransferase system (PTS). The middle domain is similar to HPr and the C-terminus is similar to the N-terminal domain of enzyme I (EI) of the PTS. The IIA domain of DhaM (via phospho-His-9), instead of ATP, is the phosphoryl donor to dihydroxyacetone (Dha). The phosphoryl flow likely involves HPr ('His-15') -> DhaM (His-432 -> His-170 -> His-9) -> DhaL-ADP -> Dha. The HPr-like domain of DhaM cannot efficiently substitute for the general carrier protein HPr.By similarity
Sequence similaritiesi
Belongs to the PEP-utilizing enzyme family.Curated
Phylogenomic databases
HOGENOMi | CLU_045361_0_0_6 |
OMAi | TDHVLVM |
Family and domain databases
CDDi | cd00367, PTS-HPr_like, 1 hit |
Gene3Di | 1.10.274.10, 1 hit 3.30.1340.10, 1 hit 3.40.50.510, 1 hit |
InterProi | View protein in InterPro IPR039643, DhaM IPR012844, DhaM_N IPR000032, HPr-like IPR035895, HPr-like_sf IPR008279, PEP-util_enz_mobile_dom IPR036637, Phosphohistidine_dom_sf IPR004701, PTS_EIIA_man-typ IPR036662, PTS_EIIA_man-typ_sf IPR008731, PTS_EIN IPR001020, PTS_HPr_His_P_site IPR036618, PtsI_HPr-bd_sf |
PANTHERi | PTHR38594, PTHR38594, 1 hit |
Pfami | View protein in Pfam PF03610, EIIA-man, 1 hit PF05524, PEP-utilisers_N, 1 hit PF00391, PEP-utilizers, 1 hit PF00381, PTS-HPr, 1 hit |
PRINTSi | PR00107, PHOSPHOCPHPR |
SUPFAMi | SSF47831, SSF47831, 1 hit SSF52009, SSF52009, 1 hit SSF53062, SSF53062, 1 hit SSF55594, SSF55594, 1 hit |
TIGRFAMsi | TIGR02364, dha_pts, 1 hit TIGR01003, PTS_HPr_family, 1 hit |
PROSITEi | View protein in PROSITE PS51096, PTS_EIIA_TYPE_4, 1 hit PS51350, PTS_HPR_DOM, 1 hit PS00369, PTS_HPR_HIS, 1 hit |
i Sequence
Sequence statusi: Complete.
A0A0H3H456-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVNLVIVSHS ARLGEGVGEL ARQMLMNDGC KLAIAAGIDD PASPIGTDPI
60 70 80 90 100
KVMEAIESVA DADHILVMMD IGSALLSAET ALDLLDPAIA AKVRLCAAPL
110 120 130 140 150
VEGTLAATVS AAAGAGIDKV IEDAMNALEA KRVQLGLPSQ PQHASLTAAP
160 170 180 190 200
VDDRDARSVS VVIQNHNGLH VRPASKLVAA LAGFNADLVL EKGGKCVTPD
210 220 230 240 250
SLNQIALLQV RRNDTLRLLA RGPDADAALA AFQALAAENF GEPTEAAPAR
260 270 280 290 300
RPASADRVEG KVVLYPQPQD RISRETSAAI GQQQLRLKRA IDRTLEDLSA
310 320 330 340 350
LTTLAEATFS ADIAAIFSGH HTLLDDPDLY AAACDIIRDE QCSAAWAWQQ
360 370 380 390 400
VLSDLSQQYR HLDDAYLQAR YIDIEDILHR TLRHLNERNE ALPQFSAPSI
410 420 430 440 450
LVADDIFPST VLQLNAEQVK GICLQAGSEL SHGAIIARQA GIAMLCQQSD
460 470
ALTLQDGENV ILDIPGKRVI RG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP003218 Genomic DNA Translation: AEX02377.1 |
RefSeqi | WP_014226903.1, NC_016612.1 |
Genome annotation databases
EnsemblBacteriai | AEX02377; AEX02377; KOX_03180 |
KEGGi | kox:KOX_03180 |
PATRICi | fig|1006551.4.peg.636 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP003218 Genomic DNA Translation: AEX02377.1 |
RefSeqi | WP_014226903.1, NC_016612.1 |
3D structure databases
SMRi | A0A0H3H456 |
ModBasei | Search... |
Genome annotation databases
EnsemblBacteriai | AEX02377; AEX02377; KOX_03180 |
KEGGi | kox:KOX_03180 |
PATRICi | fig|1006551.4.peg.636 |
Phylogenomic databases
HOGENOMi | CLU_045361_0_0_6 |
OMAi | TDHVLVM |
Family and domain databases
CDDi | cd00367, PTS-HPr_like, 1 hit |
Gene3Di | 1.10.274.10, 1 hit 3.30.1340.10, 1 hit 3.40.50.510, 1 hit |
InterProi | View protein in InterPro IPR039643, DhaM IPR012844, DhaM_N IPR000032, HPr-like IPR035895, HPr-like_sf IPR008279, PEP-util_enz_mobile_dom IPR036637, Phosphohistidine_dom_sf IPR004701, PTS_EIIA_man-typ IPR036662, PTS_EIIA_man-typ_sf IPR008731, PTS_EIN IPR001020, PTS_HPr_His_P_site IPR036618, PtsI_HPr-bd_sf |
PANTHERi | PTHR38594, PTHR38594, 1 hit |
Pfami | View protein in Pfam PF03610, EIIA-man, 1 hit PF05524, PEP-utilisers_N, 1 hit PF00391, PEP-utilizers, 1 hit PF00381, PTS-HPr, 1 hit |
PRINTSi | PR00107, PHOSPHOCPHPR |
SUPFAMi | SSF47831, SSF47831, 1 hit SSF52009, SSF52009, 1 hit SSF53062, SSF53062, 1 hit SSF55594, SSF55594, 1 hit |
TIGRFAMsi | TIGR02364, dha_pts, 1 hit TIGR01003, PTS_HPr_family, 1 hit |
PROSITEi | View protein in PROSITE PS51096, PTS_EIIA_TYPE_4, 1 hit PS51350, PTS_HPR_DOM, 1 hit PS00369, PTS_HPR_HIS, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DHAM_KLEOK | |
Accessioni | A0A0H3H456Primary (citable) accession number: A0A0H3H456 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 13, 2016 |
Last sequence update: | September 16, 2015 | |
Last modified: | February 23, 2022 | |
This is version 32 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |