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Entry version 24 (11 Dec 2019)
Sequence version 1 (16 Sep 2015)
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Protein

UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase GtfA subunit

Gene

gtfA

Organism
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Required for the polymorphic O-glycosylation of serine-rich repeat protein PsrP. Catalyzes the first step in glycosylation by transferring N-acetylglucosamine from UDP-GlcNAc to serine residues in PsrP (PubMed:24936067, PubMed:28246170). Part of the accessory SecA2/SecY2 system specifically required to export serine-rich repeat cell wall proteins encoded upstream in the same operon (Probable). The GtfA-GtfB complex adds GlcNAc from UDP-GlcNAc to PsrP (experimentally characterized with truncated PsrP-SSR1 constructs); this subunit alone has weak N-acetylglucosaminyl transferase activity that is 10-fold stimulated by GtfB. The complex requires at least a 25 residue-long peptide for activity; the in vitro assay has only been seen to glycosylate Ser residues (PubMed:24936067). The alpha linkage was shown in L.reuteri.By similarity1 Publication2 Publications

Miscellaneous

Encoded in RD10, a pathogenicity island with an atypical GC content that is associated with invasive pneumococcal disease. Pathogenicity islands account for greater than half the genomic diversity observed between isolates (PubMed:11463916, PubMed:16861665). The main function of this island seems to be correct synthesis and export of pneumococcal serine-rich repeat protein PsrP (Probable).2 PublicationsCurated

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

kcat for hydrolysis of UDP-N-acetyl-alpha-D-glucosamine with transfer of GlcNAc to PsrP-SSR1 and generation of UDP is 76.0 min(-1) for GtfA-GtfB and 7.35 min(-1) for GtfA alone.1 Publication

      <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: protein glycosylation

      This protein is involved in the pathway protein glycosylation, which is part of Protein modification.UniRule annotation2 Publications
      View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

      Sites

      Feature keyPosition(s)DescriptionActionsGraphical viewLength
      <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei242N-acetyl-D-glucosamineUniRule annotationCombined sources1 Publication1
      Binding sitei328UDPCombined sources1 Publication1
      Binding sitei332N-acetyl-D-glucosamineCombined sources1
      Binding sitei333UDPCombined sources1 Publication1
      Binding sitei358UDP; via amide nitrogenCombined sources1 Publication1

      Regions

      Feature keyPosition(s)DescriptionActionsGraphical viewLength
      <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi16 – 19UDPUniRule annotationCombined sources1 Publication4
      Nucleotide bindingi384 – 385UDPUniRule annotationCombined sources1 Publication2
      Nucleotide bindingi408 – 412UDPCombined sources1 Publication5

      <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

      GO - Biological processi

      <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

      Molecular functionGlycosyltransferase, Transferase
      LigandNucleotide-binding

      Enzyme and pathway databases

      BioCyc Collection of Pathway/Genome Databases

      More...
      BioCyci
      SPNE170187:G1FZB-1783-MONOMER

      UniPathway: a resource for the exploration and annotation of metabolic pathways

      More...
      UniPathwayi
      UPA00378

      <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

      <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
      Recommended name:
      UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase GtfA subunitUniRule annotationCurated (EC:2.4.1.-UniRule annotation1 Publication)
      Alternative name(s):
      Glycosyltransferase GtfA1 PublicationUniRule annotation
      O-linked N-acetyl-D-glucosamine (O-GlcNAc) transferase1 Publication
      <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
      Name:gtfA1 PublicationUniRule annotation
      Ordered Locus Names:SP_1758
      <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiStreptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
      <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri170187 [NCBI]
      <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
      <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
      • UP000000585 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

      <p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

      GO - Cellular componenti

      Keywords - Cellular componenti

      Cell membrane, Cytoplasm, Membrane

      <p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

      Mutagenesis

      Feature keyPosition(s)DescriptionActionsGraphical viewLength
      <p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi98N → A: Decreased N-acetylglucosaminyl transferase activity, decreased binding of PsrP. 1 Publication1
      Mutagenesisi103R → A: Decreased N-acetylglucosaminyl transferase activity, decreased binding of PsrP. 1 Publication1
      Mutagenesisi116Y → A: Decreased N-acetylglucosaminyl transferase activity, decreased binding of PsrP. 1 Publication1
      Mutagenesisi244E → A: Loss of N-acetylglucosaminyl transferase activity. 1 Publication1
      Mutagenesisi328R → A: Loss of N-acetylglucosaminyl transferase activity. 1 Publication1
      Mutagenesisi332E → A: Loss of N-acetylglucosaminyl transferase activity, nearly wild-type binding of PsrP. 1 Publication1
      Mutagenesisi333K → A: Loss of N-acetylglucosaminyl transferase activity. 1 Publication1
      Mutagenesisi403S → A: Significant loss of N-acetylglucosaminyl transferase activity. 1 Publication1

      <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

      Molecule processing

      Feature keyPosition(s)DescriptionActionsGraphical viewLength
      <p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004472021 – 503UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase GtfA subunitAdd BLAST503

      <p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

      <p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

      Monomer (PubMed:24936067).

      Interacts with stabilizing protein GtfB, probably as a heterotetramer with 2 subunits each of GtfA and GtfB, part of the accessory SecA2/SecY2 protein translocation apparatus (PubMed:24936067).

      1 Publication

      <p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

      Secondary structure

      1503
      Legend: HelixTurnBeta strandPDB Structure known for this area
      Show more details

      3D structure databases

      SWISS-MODEL Repository - a database of annotated 3D protein structure models

      More...
      SMRi
      A0A0H2URG7

      Database of comparative protein structure models

      More...
      ModBasei
      Search...

      Protein Data Bank in Europe - Knowledge Base

      More...
      PDBe-KBi
      Search...

      <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

      Region

      Feature keyPosition(s)DescriptionActionsGraphical viewLength
      <p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1 – 78N-terminus R-fold-11 PublicationAdd BLAST78
      Regioni79 – 195Extended beta-sheet domain1 PublicationAdd BLAST117
      Regioni196 – 306C-terminus R-fold-11 PublicationAdd BLAST111
      Regioni307 – 503R-fold-21 PublicationAdd BLAST197
      Regioni404 – 407N-acetyl-D-glucosamine bindingUniRule annotationCombined sources1 Publication4

      <p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

      Has 2 Rossmann-like domains, called R-fold-1 and R-fold-2. R-fold-1 is interrupted by an extended beta-sheet domain that loops out of the structure and is required for recognizing both PsrP substrate and coactivator GtfB. UDP and N-acetyl-D-glucosamine bind between the Rossmann-like folds.1 Publication

      <p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

      Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.UniRule annotation

      Phylogenomic databases

      KEGG Orthology (KO)

      More...
      KOi
      K00712

      Identification of Orthologs from Complete Genome Data

      More...
      OMAi
      HIDWLVA

      Family and domain databases

      HAMAP database of protein families

      More...
      HAMAPi
      MF_01472 GtfA, 1 hit

      Integrated resource of protein families, domains and functional sites

      More...
      InterProi
      View protein in InterPro
      IPR001296 Glyco_trans_1
      IPR014267 Gtf1

      Pfam protein domain database

      More...
      Pfami
      View protein in Pfam
      PF00534 Glycos_transf_1, 1 hit

      TIGRFAMs; a protein family database

      More...
      TIGRFAMsi
      TIGR02918 TIGR02918, 1 hit

      <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

      <p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

      A0A0H2URG7-1 [UniParc]FASTAAdd to basket
      « Hide
              10         20         30         40         50
      MTIYNINLGI GWASSGVEYA QAYRAGVFRK LNLSSKFIFT DMILADNIQH
      60 70 80 90 100
      LTANIGFDDN QVIWLYNHFT DIKIAPTSVT VDDVLAYFGG EESHREKNGK
      110 120 130 140 150
      VLRVFFFDQD KFVTCYLVDE NKDLVQHAEY VFKGNLIRKD YFSYTRYCSE
      160 170 180 190 200
      YFAPKDNVAV LYQRTFYNED GTPVYDILMN QGKEEVYHFK DKIFYGKQAF
      210 220 230 240 250
      VRAFMKSLNL NKSDLVILDR ETGIGQVVFE EAQTAHLAVV VHAEHYSENA
      260 270 280 290 300
      TNEDYILWNN YYDYQFTNAD KVDFFIVSTD RQNEVLQEQF AKYTQHQPKI
      310 320 330 340 350
      VTIPVGSIDS LTDSSQGRKP FSLITASRLA KEKHIDWLVK AVIEAHKELP
      360 370 380 390 400
      ELTFDIYGSG GEDSLLREII ANHQAEDYIQ LKGHAELSQI YSQYEVYLTA
      410 420 430 440 450
      STSEGFGLTL MEAIGSGLPL IGFDVPYGNQ TFIEDGQNGY LIPSSSDHVE
      460 470 480 490 500
      DQIKQAYAAK ICQLYQENRL EAMRAYSYQI AEGFLTKEIL EKWKKTVEEV

      LHD
      Length:503
      Mass (Da):57,782
      Last modified:September 16, 2015 - v1
      <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i128E7F4F393B7084
      GO

      Sequence databases

      Select the link destinations:

      EMBL nucleotide sequence database

      More...
      EMBLi

      GenBank nucleotide sequence database

      More...
      GenBanki

      DNA Data Bank of Japan; a nucleotide sequence database

      More...
      DDBJi
      Links Updated
      AE005672 Genomic DNA Translation: AAK75833.1

      NCBI Reference Sequences

      More...
      RefSeqi
      WP_000158460.1, NZ_AKVY01000001.1

      Genome annotation databases

      Ensembl bacterial and archaeal genome annotation project

      More...
      EnsemblBacteriai
      AAK75833; AAK75833; SP_1758

      KEGG: Kyoto Encyclopedia of Genes and Genomes

      More...
      KEGGi
      spn:SP_1758

      <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

      <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

      Sequence databases

      Select the link destinations:
      EMBLi
      GenBanki
      DDBJi
      Links Updated
      AE005672 Genomic DNA Translation: AAK75833.1
      RefSeqiWP_000158460.1, NZ_AKVY01000001.1

      3D structure databases

      Select the link destinations:

      Protein Data Bank Europe

      More...
      PDBei

      Protein Data Bank RCSB

      More...
      RCSB PDBi

      Protein Data Bank Japan

      More...
      PDBji
      Links Updated
      PDB entryMethodResolution (Å)ChainPositionsPDBsum
      4PQGX-ray2.00A/B1-503[»]
      SMRiA0A0H2URG7
      ModBaseiSearch...
      PDBe-KBiSearch...

      Genome annotation databases

      EnsemblBacteriaiAAK75833; AAK75833; SP_1758
      KEGGispn:SP_1758

      Phylogenomic databases

      KOiK00712
      OMAiHIDWLVA

      Enzyme and pathway databases

      UniPathwayiUPA00378
      BioCyciSPNE170187:G1FZB-1783-MONOMER

      Family and domain databases

      HAMAPiMF_01472 GtfA, 1 hit
      InterProiView protein in InterPro
      IPR001296 Glyco_trans_1
      IPR014267 Gtf1
      PfamiView protein in Pfam
      PF00534 Glycos_transf_1, 1 hit
      TIGRFAMsiTIGR02918 TIGR02918, 1 hit

      ProtoNet; Automatic hierarchical classification of proteins

      More...
      ProtoNeti
      Search...

      MobiDB: a database of protein disorder and mobility annotations

      More...
      MobiDBi
      Search...

      <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

      <p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiGTFA_STRPN
      <p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: A0A0H2URG7
      <p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 8, 2019
      Last sequence update: September 16, 2015
      Last modified: December 11, 2019
      This is version 24 of the entry and version 1 of the sequence. See complete history.
      <p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
      Annotation programProkaryotic Protein Annotation Program

      <p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

      Keywords - Technical termi

      3D-structure

      Documents

      1. PATHWAY comments
        Index of metabolic and biosynthesis pathways
      2. PDB cross-references
        Index of Protein Data Bank (PDB) cross-references
      3. SIMILARITY comments
        Index of protein domains and families
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