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Protein

Inosine-5'-monophosphate dehydrogenase

Gene

guaB

Organism
Bacillus anthracis
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Catalytic activityi

Inosine 5'-phosphate + NAD+ + H2O = xanthosine 5'-phosphate + NADH.UniRule annotation

Cofactori

K+UniRule annotation

Enzyme regulationi

Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.UniRule annotation

Pathwayi: XMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes XMP from IMP.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Inosine-5'-monophosphate dehydrogenase (guaB), Inosine-5'-monophosphate dehydrogenase (guaB), Inosine-5'-monophosphate dehydrogenase (guaB), Inosine-5'-monophosphate dehydrogenase (guaB), Inosine-5'-monophosphate dehydrogenase (guaB), Inosine-5'-monophosphate dehydrogenase (guaB), Inosine-5'-monophosphate dehydrogenase (guaB), Inosine-5'-monophosphate dehydrogenase (guaB), Inosine-5'-monophosphate dehydrogenase (guaB), Inosine-5'-monophosphate dehydrogenase (guaB)
This subpathway is part of the pathway XMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes XMP from IMP, the pathway XMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei256NADUniRule annotation1
Metal bindingi308Potassium; via carbonyl oxygenUniRule annotation1
Metal bindingi310Potassium; via carbonyl oxygenUniRule annotation1
Binding sitei311IMPUniRule annotation1
Active sitei313Thioimidate intermediateUniRule annotation1
Metal bindingi313Potassium; via carbonyl oxygenUniRule annotation1
Active sitei409Proton acceptorUniRule annotation1
Binding sitei421IMPUniRule annotation1
Metal bindingi475Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation1
Metal bindingi476Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation1
Metal bindingi477Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi256 – 258NADUniRule annotation3
Nucleotide bindingi306 – 308NADUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductaseUniRule annotation
Biological processGMP biosynthesisUniRule annotation, Purine biosynthesis
LigandMetal-bindingUniRule annotation, NADUniRule annotation, PotassiumUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00601; UER00295

Names & Taxonomyi

Protein namesi
Recommended name:
Inosine-5'-monophosphate dehydrogenaseUniRule annotation (EC:1.1.1.205UniRule annotation)
Short name:
IMP dehydrogenaseUniRule annotation
Short name:
IMPDUniRule annotation
Short name:
IMPDHUniRule annotation
Gene namesi
Name:guaBUniRule annotationImported
ORF Names:BASH2_00239Imported
OrganismiBacillus anthracisImported
Taxonomic identifieri1392 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000217643 Componenti: Chromosome

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA0A0F7R3F1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini100 – 158CBSInterPro annotationAdd BLAST59
Domaini162 – 220CBSInterPro annotationAdd BLAST59

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni346 – 348IMP bindingUniRule annotation3
Regioni369 – 370IMP bindingUniRule annotation2
Regioni393 – 397IMP bindingUniRule annotation5

Sequence similaritiesi

Belongs to the IMPDH/GMPR family.UniRule annotation

Keywords - Domaini

CBS domainPROSITE-ProRule annotation

Family and domain databases

CDDicd00381 IMPDH, 1 hit
Gene3Di3.20.20.70, 1 hit
HAMAPiMF_01964 IMPDH, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR000644 CBS_dom
IPR005990 IMP_DH
IPR015875 IMP_DH/GMP_Rdtase_CS
IPR001093 IMP_DH_GMPRt
PfamiView protein in Pfam
PF00571 CBS, 2 hits
PF00478 IMPDH, 1 hit
PIRSFiPIRSF000130 IMPDH, 1 hit
SMARTiView protein in SMART
SM00116 CBS, 2 hits
TIGRFAMsiTIGR01302 IMP_dehydrog, 1 hit
PROSITEiView protein in PROSITE
PS51371 CBS, 2 hits
PS00487 IMP_DH_GMP_RED, 1 hit

Sequencei

Sequence statusi: Complete.

A0A0F7R3F1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGGTIMWESK FVKEGLTFDD VLLVPAKSDV LPREVSVKTV LSESLQLNIP
60 70 80 90 100
LISAGMDTVT EADMAIAMAR QGGLGIIHKN MSIEQQAEQV DKVKRSESGV
110 120 130 140 150
ISDPFFLTPE HQVYDAEHLM GKYRISGVPV VNNLDERKLV GIITNRDMRF
160 170 180 190 200
IQDYSIKISD VMTKEQLITA PVGTTLSEAE KILQKYKIEK LPLVDNNGVL
210 220 230 240 250
QGLITIKDIE KVIEFPNSAK DKQGRLLVGA AVGVTADAMT RIDALVKASV
260 270 280 290 300
DAIVLDTAHG HSQGVIDKVK EVRAKYPSLN IIAGNVATAE ATKALIEAGA
310 320 330 340 350
NVVKVGIGPG SICTTRVVAG VGVPQLTAVY DCATEARKHG IPVIADGGIK
360 370 380 390 400
YSGDMVKALA AGAHVVMLGS MFAGVAESPG ETEIYQGRQF KVYRGMGSVG
410 420 430 440 450
AMEKGSKDRY FQEGNKKLVP EGIEGRVPYK GPLADTVHQL VGGLRAGMGY
460 470 480 490
CGAQDLEFLR ENAQFIRMSG AGLLESHPHH VQITKEAPNY SL
Length:492
Mass (Da):52,834
Last modified:July 22, 2015 - v1
Checksum:i2C51A6F06B9CC73C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP014833 Genomic DNA Translation: BAR73646.1

Genome annotation databases

PATRICifig|1392.236.peg.10

Similar proteinsi

Entry informationi

Entry nameiA0A0F7R3F1_BACAN
AccessioniPrimary (citable) accession number: A0A0F7R3F1
Entry historyiIntegrated into UniProtKB/TrEMBL: July 22, 2015
Last sequence update: July 22, 2015
Last modified: March 28, 2018
This is version 16 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

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