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Protein

Aspartate--tRNA(Asp/Asn) ligase

Gene

aspS

Organism
Halanaeroarchaeum sulfurireducens
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn).UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Catalytic activityi

ATP + L-aspartate + tRNA(Asx) = AMP + diphosphate + L-aspartyl-tRNA(Asx).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 3 Mg2+ cations per subunit. The strongest magnesium site (Mg1) is bound to the beta- and gamma-phosphates of ATP and four water molecules complete its coordination sphere.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei95Important for tRNA non-discriminationUniRule annotation1
Binding sitei180AspartateUniRule annotation1
Binding sitei224AspartateUniRule annotation1
Metal bindingi370Magnesium 2UniRule annotation1
Metal bindingi370Magnesium 3UniRule annotation1
Binding sitei370ATPUniRule annotation1
Metal bindingi373Magnesium 2UniRule annotation1
Binding sitei373AspartateUniRule annotation1
Binding sitei377AspartateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi224 – 226ATPUniRule annotation3
Nucleotide bindingi232 – 234ATPUniRule annotation3
Nucleotide bindingi418 – 421ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetaseUniRule annotationImported, Ligase
Biological processProtein biosynthesisUniRule annotation
LigandATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate--tRNA(Asp/Asn) ligaseUniRule annotation (EC:6.1.1.23UniRule annotation)
Alternative name(s):
Aspartyl-tRNA synthetaseUniRule annotation
Short name:
AspRSUniRule annotation
Non-discriminating aspartyl-tRNA synthetaseUniRule annotation
Short name:
ND-AspRSUniRule annotation
Gene namesi
Name:aspSUniRule annotationImported
ORF Names:HLASF_0582Imported
OrganismiHalanaeroarchaeum sulfurireducensImported
Taxonomic identifieri1604004 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHalanaeroarchaeum
Proteomesi
  • UP000069906 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini147 – 447AA_TRNA_LIGASE_IIInterPro annotationAdd BLAST301

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni202 – 205AspartateUniRule annotation4

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family. Type 2 subfamily.UniRule annotation

Phylogenomic databases

KOiK09759
OrthoDBiPOG093Z02FK

Family and domain databases

HAMAPiMF_02075 Asp_tRNA_synth_type2, 1 hit
InterProiView protein in InterPro
IPR004364 aa-tRNA-synt_II
IPR006195 aa-tRNA-synth_II
IPR004523 Asp-tRNA_synthase_2
IPR002312 Asp/Asn-tRNA-synth_IIb
IPR012340 NA-bd_OB-fold
IPR004365 NA-bd_OB_tRNA
PANTHERiPTHR43450 PTHR43450, 1 hit
PfamiView protein in Pfam
PF00152 tRNA-synt_2, 1 hit
PF01336 tRNA_anti-codon, 1 hit
PRINTSiPR01042 TRNASYNTHASP
SUPFAMiSSF50249 SSF50249, 1 hit
TIGRFAMsiTIGR00458 aspS_nondisc, 1 hit
PROSITEiView protein in PROSITE
PS50862 AA_TRNA_LIGASE_II, 1 hit

Sequencei

Sequence statusi: Complete.

A0A0F7PCJ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAFKAAPPI PGSMQDRTFT ALAEAGDDVT VAGWVHEVRD LGGIAFLILR
60 70 80 90 100
DKAGKIQVKF EKDELDDELV ETGLSVHRES VIRVSGSVEE EPRAPTGVEV
110 120 130 140 150
TPDTLEVVAE ADPELPLDPS GKVDAELSTR LDNRTLDLRK DEPKAIFEIR
160 170 180 190 200
AAVLQAVREA FRDHGSTEIN TPKIVATGTE GGTELFPITY FGQEAFMNQS
210 220 230 240 250
PQLFKQLMVG SGLERVFEIG PIFRAEEHNT PRHLNEATSI DFESAFIDAD
260 270 280 290 300
GAMDVLEAVV TAAYEGVWEN AQPELEALDR ADDFTVPDGE FERIPYEEII
310 320 330 340 350
EMVNATGALD EQLVWGDDLP TEAEKALGEE LGEHYFITNW PSEVKPFYIK
360 370 380 390 400
DTDDDPAVST GFDLMHPRLE LVSGGQREHR YEKLIAGFEQ QGLDPEAFEY
410 420 430 440
YTKMFRYGMP PHAGWGLGAE RLIMTMLDLD NIREAVIFPR DRQRLSP
Length:447
Mass (Da):49,785
Last modified:July 22, 2015 - v1
Checksum:iD64564623143FC23
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP008874 Genomic DNA Translation: AKH97078.1

Genome annotation databases

EnsemblBacteriaiAKH97078; AKH97078; HLASF_0582
KEGGihsu:HLASF_0582
PATRICifig|1604004.4.peg.610

Similar proteinsi

Entry informationi

Entry nameiA0A0F7PCJ1_9EURY
AccessioniPrimary (citable) accession number: A0A0F7PCJ1
Entry historyiIntegrated into UniProtKB/TrEMBL: July 22, 2015
Last sequence update: July 22, 2015
Last modified: May 23, 2018
This is version 19 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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