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Protein

DNA topoisomerase 3

Gene

topB

Organism
Shigella flexneri 5a str. M90T
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds two Mg2+ per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi26Magnesium 1; catalyticUniRule annotation1
Metal bindingi122Magnesium 1; catalyticUniRule annotation1
Metal bindingi122Magnesium 2UniRule annotation1
Metal bindingi124Magnesium 2UniRule annotation1
Active sitei347O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-bindingUniRule annotation, Isomerase, TopoisomeraseUniRule annotation
LigandMagnesiumUniRule annotation, Metal-bindingUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 3UniRule annotation (EC:5.99.1.2UniRule annotation)
Alternative name(s):
DNA topoisomerase IIIUniRule annotation
Gene namesi
Name:topBUniRule annotationImported
ORF Names:SF5M90T_1421Imported
OrganismiShigella flexneri 5a str. M90TImported
Taxonomic identifieri1086030 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeShigella
Proteomesi
  • UP000005108 Componenti: Chromosome

Interactioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei80Interaction with DNAUniRule annotation1
Sitei189Interaction with DNAUniRule annotation1
Sitei197Interaction with DNAUniRule annotation1
Sitei204Interaction with DNAUniRule annotation1
Sitei349Interaction with DNAUniRule annotation1

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 153ToprimInterPro annotationAdd BLAST134

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni213 – 218Interaction with DNAUniRule annotation6

Sequence similaritiesi

Belongs to the type IA topoisomerase family.UniRule annotation

Family and domain databases

CDDicd00186 TOP1Ac, 1 hit
cd03362 TOPRIM_TopoIA_TopoIII, 1 hit
Gene3Di1.10.290.10, 1 hit
1.10.460.10, 2 hits
2.70.20.10, 2 hits
HAMAPiMF_00953 Topoisom_3_prok, 1 hit
InterProiView protein in InterPro
IPR000380 Topo_IA
IPR003601 Topo_IA_2
IPR023406 Topo_IA_AS
IPR013497 Topo_IA_cen
IPR013824 Topo_IA_cen_sub1
IPR013825 Topo_IA_cen_sub2
IPR013826 Topo_IA_cen_sub3
IPR023405 Topo_IA_core_domain
IPR003602 Topo_IA_DNA-bd_dom
IPR005738 TopoIII
IPR006171 TOPRIM_domain
IPR034144 TOPRIM_TopoIII
PANTHERiPTHR11390 PTHR11390, 1 hit
PfamiView protein in Pfam
PF01131 Topoisom_bac, 1 hit
PF01751 Toprim, 1 hit
PRINTSiPR00417 PRTPISMRASEI
SMARTiView protein in SMART
SM00437 TOP1Ac, 1 hit
SM00436 TOP1Bc, 1 hit
SM00493 TOPRIM, 1 hit
SUPFAMiSSF56712 SSF56712, 1 hit
TIGRFAMsiTIGR01056 topB, 1 hit
PROSITEiView protein in PROSITE
PS00396 TOPOISOMERASE_I_PROK, 1 hit
PS50880 TOPRIM, 1 hit

Sequencei

Sequence statusi: Complete.

A0A0F6MDT6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANWCQRAAV VPWLRSVNLM RLFIAEKPSL ARAIADVLPK PHRKGDGFIE
60 70 80 90 100
CGNGQVVTWC IGHLLEQAQP DAYDSRYARW NLADLPIVPE KWQLQPRPSV
110 120 130 140 150
TKQLNVIKRF LHEASEIVHA GDPDREGQLL VDEVLDYLQL APEKRQQVQR
160 170 180 190 200
CLINDLNPQA VERAIDRLRS NSEFVPLCVS ALARARADWL YGINMTRAYT
210 220 230 240 250
ILGRNAGYQG VLSVGRVQTP VLGLVVRRDE EIENFVAKDF FEVKAHIVTP
260 270 280 290 300
ADERFTAIWQ PSEACEPYQD EEGRLLHRPL AEHVVNRISG QPAIVTSYND
310 320 330 340 350
KRESESAPLP FSLSALQIEA AKRFGLSAQN VLDICQKLYE THKLITYPRS
360 370 380 390 400
DCRYLPEEHF AGRHAVMNAI SVHAPDLLPQ PVVDPDIRNR CWDDKKVDAH
410 420 430 440 450
HAIIPTARSS AINLTENEAK VYNLIARQYL MQFCPDAVFR KCVIELDIAK
460 470 480 490 500
GKFVAKARFL AEAGWRTLLG SKERDEENDG TPLPVVAKGD ELLCEKGEVV
510 520 530 540 550
ERQTQPPRHF TDATLLSAMT GIARFVQDKD LKKVLRATDG LGTEATRAGI
560 570 580 590 600
IELLFKRGFL TKKGRYIHST DAGKALFHSL PEMATRPDMT AHWESVLTQI
610 620 630 640 650
SEKQCRYQDF MQPLVGTLYQ LIDQAKRTPV RQFRGIVAPG SGGSADKKKA
660 670
APRKRSAKKS PPADEAGSGA IA
Length:672
Mass (Da):75,371
Last modified:June 24, 2015 - v1
Checksum:iF0C07F7C2E93A7C6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CM001474 Genomic DNA Translation: EID61936.1

Genome annotation databases

EnsemblBacteriaiEID61936; EID61936; SF5M90T_1421

Similar proteinsi

Entry informationi

Entry nameiA0A0F6MDT6_SHIFL
AccessioniPrimary (citable) accession number: A0A0F6MDT6
Entry historyiIntegrated into UniProtKB/TrEMBL: June 24, 2015
Last sequence update: June 24, 2015
Last modified: February 28, 2018
This is version 18 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

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