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Protein

Glyceraldehyde-3-phosphate dehydrogenase

Gene

gap

Organism
Methanosarcina barkeri 227
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+ = 3-phospho-D-glyceroyl phosphate + NAD(P)H.UniRule annotationSAAS annotation

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.UniRule annotationSAAS annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gap), Glyceraldehyde-3-phosphate dehydrogenase (gap)
  2. Phosphoglycerate kinase (pgk), Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (apgM), 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA)
  4. Enolase (eno)
  5. Pyruvate kinase (MSBR2_2739)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei111NAD; via amide nitrogenUniRule annotation1
Active sitei141NucleophileUniRule annotation1
Binding sitei169NADUniRule annotation1
Binding sitei300NAD; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi13 – 14NADUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductaseUniRule annotationSAAS annotationImported
Biological processGlycolysisUniRule annotationSAAS annotation
LigandNADUniRule annotationSAAS annotation, NADPUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayi
UPA00109;UER00184

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenaseUniRule annotation (EC:1.2.1.59UniRule annotation)
Short name:
GAPDHUniRule annotation
Alternative name(s):
NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenaseUniRule annotation
Gene namesi
Name:gapUniRule annotation
ORF Names:MSBR2_0489Imported
OrganismiMethanosarcina barkeri 227Imported
Taxonomic identifieri1434106 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
Proteomesi
  • UP000033079 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 141Gp_dh_NInterPro annotationAdd BLAST138

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni140 – 142Glyceraldehyde 3-phosphate bindingUniRule annotation3
Regioni195 – 196Glyceraldehyde 3-phosphate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.UniRule annotationSAAS annotation

Phylogenomic databases

KOiK00150
OrthoDBiPOG093Z05Z6

Family and domain databases

HAMAPiMF_00559 G3P_dehdrog_arch, 1 hit
InterProiView protein in InterPro
IPR000846 DapB_N
IPR020831 GlycerAld/Erythrose_P_DH
IPR020830 GlycerAld_3-P_DH_AS
IPR020829 GlycerAld_3-P_DH_cat
IPR020828 GlycerAld_3-P_DH_NAD(P)-bd
IPR006436 Glyceraldehyde-3-P_DH_2_arc
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR10836 PTHR10836, 1 hit
PTHR10836:SF76 PTHR10836:SF76, 1 hit
PfamiView protein in Pfam
PF01113 DapB_N, 1 hit
PF02800 Gp_dh_C, 1 hit
PIRSFiPIRSF000149 GAP_DH, 1 hit
SMARTiView protein in SMART
SM00846 Gp_dh_N, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR01546 GAPDH-II_archae, 1 hit
PROSITEiView protein in PROSITE
PS00071 GAPDH, 1 hit

Sequencei

Sequence statusi: Complete.

A0A0E3R0S2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAKAKIAVNG YGTIGKRVAD AVRAQDDMEV VGISKTKPNY EAAVAHKLGY
60 70 80 90 100
DIYAPAENVE TFEKAGMPAA GSIEEMLEKA DLVVDCTPGG IGEKNKPMYE
110 120 130 140 150
KIGIKAIWQG GESHPLAGFS FNAESNYEQA VGRDLVRVVS CNTTALCRAI
160 170 180 190 200
STIDREFGVN KVRVSLTRRA VDPNEIKKGP VDAIVLNPIK LPSHHGPDVQ
210 220 230 240 250
SVLPHINITT AAIKVPTTLM HVHTVNMEVN KDCTAEDVKN IFGSQSRIRL
260 270 280 290 300
VGQGITSTAE IIEFARDIGR PRHDMWELCI WPESITVTDK ELYFFHAVHQ
310 320 330
ESIVVPENVD AIRAMMELES DGAKSIEKTN KAIGLYNK
Length:338
Mass (Da):37,006
Last modified:June 24, 2015 - v1
Checksum:i5E75F6A6EA50AA6F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP009530 Genomic DNA Translation: AKB57005.1
RefSeqiWP_048117217.1, NZ_CP009530.1

Genome annotation databases

EnsemblBacteriaiAKB57005; AKB57005; MSBR2_0489
GeneIDi24884155
KEGGimbar:MSBR2_0489
PATRICifig|1434106.5.peg.595

Similar proteinsi

Entry informationi

Entry nameiA0A0E3R0S2_METBA
AccessioniPrimary (citable) accession number: A0A0E3R0S2
Entry historyiIntegrated into UniProtKB/TrEMBL: June 24, 2015
Last sequence update: June 24, 2015
Last modified: September 12, 2018
This is version 22 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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