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Protein

Polyribonucleotide 5'-hydroxyl-kinase Clp1

Gene

CLP1

Organism
Chlorocebus sabaeus (Green monkey) (Cercopithecus sabaeus)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Appears to have roles in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex. Phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA. Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However, endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing.UniRule annotation

Catalytic activityi

ATP + 5'-dephospho-DNA = ADP + 5'-phospho-DNA.UniRule annotation
ATP + 5'-dephospho-RNA = ADP + 5'-phospho-RNA.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotation, Ni2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei22ATPUniRule annotation1
Binding sitei62ATP; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi124 – 129ATPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinaseUniRule annotation, Transferase
Biological processmRNA processingUniRule annotationSAAS annotation, tRNA processingUniRule annotation
LigandATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotation, ManganeseUniRule annotation, NickelUniRule annotation, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide 5'-hydroxyl-kinase Clp1UniRule annotation (EC:2.7.1.78UniRule annotation)
Alternative name(s):
Polyadenylation factor Clp1UniRule annotation
Polynucleotide kinase Clp1UniRule annotation
Pre-mRNA cleavage complex II protein Clp1UniRule annotation
Gene namesi
Name:CLP1UniRule annotationImported
OrganismiChlorocebus sabaeus (Green monkey) (Cercopithecus sabaeus)Imported
Taxonomic identifieri60711 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeChlorocebus
Proteomesi
  • UP000029965 Componenti: Chromosome 1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

NucleusUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Component of the tRNA splicing endonuclease complex, composed of CLP1, TSEN2, TSEN15, TSEN34 and TSEN54. Component of pre-mRNA cleavage complex II (CF-II). Also associates with numerous components of the pre-mRNA cleavage complex I (CF-I/CFIm), including NUDT21, CPSF2, CPSF3, CPSF6 and CPSF7. Interacts with CSTF2 and SYMPK.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA0A0D9R107
SMRiA0A0D9R107
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini16 – 107CLP1_NInterPro annotationAdd BLAST92
Domaini121 – 307CLP1_PInterPro annotationAdd BLAST187
Domaini313 – 423Clp1InterPro annotationAdd BLAST111

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili93 – 113Sequence analysisAdd BLAST21

Sequence similaritiesi

Belongs to the Clp1 family. Clp1 subfamily.UniRule annotationSAAS annotation

Keywords - Domaini

Coiled coilSequence analysis

Phylogenomic databases

GeneTreeiENSGT00390000000344
KOiK14399
OMAiKVAIFTY

Family and domain databases

Gene3Di2.60.120.1030, 1 hit
3.30.70.2410, 1 hit
HAMAPiMF_03035 Clp1, 1 hit
InterProiView protein in InterPro
IPR028606 Clp1
IPR010655 Clp1_C
IPR038238 Clp1_C_sf
IPR032324 Clp1_N
IPR038239 Clp1_N_sf
IPR032319 CLP1_P
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF06807 Clp1, 1 hit
PF16573 CLP1_N, 1 hit
PF16575 CLP1_P, 1 hit
SUPFAMiSSF52540 SSF52540, 3 hits

Sequencei

Sequence statusi: Complete.

A0A0D9R107-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGEEANDDKK PTTKFELERE TELRFEVEAS QSVQLELLTG MAEIFGTELT
60 70 80 90 100
RNKKFTFDAG AKVAVFTWHG CSVQLSGRTE VAYVSKDTPM LLYLNTHTAL
110 120 130 140 150
EQMRRQAEKE EERGPRVMVV GPTDVGKSTV CRLLLNYAVR LGRRPTYVEL
160 170 180 190 200
DVGQGSVSIP GTMGALYIER PADVEEGFSI QAPLVYHFGS TTPGTNIKLY
210 220 230 240 250
NKITSRLADV FNQRCEVNRR ASVSGCVINT CGWVKGSGYQ ALVHAASAFE
260 270 280 290 300
VDVVVVLDQE RLYNELKRDL PHFVRTVLLP KSGGVVERSK DFRRECRDER
310 320 330 340 350
IREYFYGFRG CFYPHAFNVK FSDVKIYKVG APTIPDSCLP LGMSQEDNQL
360 370 380 390 400
KLVPVTPGRD MVHHLLSVST AEGTEENLSE TSVAGFIVVT SVDLEHQVFT
410 420
VLSPAPRPLP KNFLLIMDIR FMDLK
Length:425
Mass (Da):47,646
Last modified:May 27, 2015 - v1
Checksum:i640AF4768994CFE6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AQIB01080297 Genomic DNA No translation available.
RefSeqiXP_007996426.1, XM_007998235.1

Genome annotation databases

EnsembliENSCSAT00000004029; ENSCSAP00000002296; ENSCSAG00000005994
GeneIDi103235350
KEGGicsab:103235350

Similar proteinsi

Entry informationi

Entry nameiA0A0D9R107_CHLSB
AccessioniPrimary (citable) accession number: A0A0D9R107
Entry historyiIntegrated into UniProtKB/TrEMBL: May 27, 2015
Last sequence update: May 27, 2015
Last modified: March 28, 2018
This is version 24 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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