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Protein

Fumarate hydratase class II

Gene

fumC

Organism
Bacillus amyloliquefaciens (Bacillus velezensis)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.UniRule annotation

Miscellaneous

There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.UniRule annotation

Catalytic activityi

(S)-malate = fumarate + H2O.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from fumarate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from fumarate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei185SubstrateUniRule annotation1
Active sitei186Proton donor/acceptorUniRule annotation1
Active sitei316UniRule annotation1
Binding sitei317SubstrateUniRule annotation1
Sitei329Important for catalytic activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseUniRule annotationSAAS annotation
Biological processTricarboxylic acid cycleUniRule annotation

Enzyme and pathway databases

UniPathwayi
UPA00223;UER01007

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate hydratase class IIUniRule annotation (EC:4.2.1.2UniRule annotation)
Short name:
Fumarase CUniRule annotation
Alternative name(s):
Aerobic fumaraseUniRule annotation
Iron-independent fumaraseUniRule annotation
Gene namesi
Name:fumCUniRule annotationImported
ORF Names:BAALB65_16110Imported, DDT09_15950Imported, UZ38_06110Imported
OrganismiBacillus amyloliquefaciens (Bacillus velezensis)Imported
Taxonomic identifieri1390 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus amyloliquefaciens group
Proteomesi
  • UP000246060 Componenti: Chromosome
  • UP000032472 Componenti: Unassembled WGS sequence
  • UP000245421 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi326423.RBAM_030160

Structurei

3D structure databases

ProteinModelPortaliA0A0D7XTY8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 340Lyase_1InterPro annotationAdd BLAST330
Domaini406 – 459FumaraseC_CInterPro annotationAdd BLAST54

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni97 – 99Substrate bindingUniRule annotation3
Regioni127 – 130Substrate binding (B site)UniRule annotation4
Regioni137 – 139Substrate bindingUniRule annotation3
Regioni322 – 324Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.UniRule annotation

Family and domain databases

CDDicd01362 Fumarase_classII, 1 hit
Gene3Di1.10.275.10, 1 hit
HAMAPiMF_00743 FumaraseC, 1 hit
InterProiView protein in InterPro
IPR005677 Fum_hydII
IPR024083 Fumarase/histidase_N
IPR018951 Fumarase_C_C
IPR020557 Fumarate_lyase_CS
IPR000362 Fumarate_lyase_fam
IPR022761 Fumarate_lyase_N
IPR008948 L-Aspartase-like
PANTHERiPTHR11444 PTHR11444, 1 hit
PfamiView protein in Pfam
PF10415 FumaraseC_C, 1 hit
PF00206 Lyase_1, 1 hit
PRINTSiPR00149 FUMRATELYASE
SUPFAMiSSF48557 SSF48557, 1 hit
TIGRFAMsiTIGR00979 fumC_II, 1 hit
PROSITEiView protein in PROSITE
PS00163 FUMARATE_LYASES, 1 hit

Sequencei

Sequence statusi: Complete.

A0A0D7XTY8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDYRIEKDTM GEVKVPADKF WGAQTQRSKE NFKIGSEKMP QRVVKAFAIL
60 70 80 90 100
KRSTAIANER LGNLESEKAQ AIAAVCDDVL NGKYDDNFPL VVWQTGSGTQ
110 120 130 140 150
SNMNMNEVVA NRATALLKEK NSELKIHPND DVNRSQSSND TFPTAMHVAA
160 170 180 190 200
VLAVYEQLVP ALDQLRATLD EKAKAYQDIV KIGRTHLQDA TPLTLGQEIS
210 220 230 240 250
GWVYMLDRSK EMILESTEKI RALAIGGTAV GTGINAHPQF GEFVAEEITK
260 270 280 290 300
VTGQTFKSSP NKFHALTSHD EITHVHGALK ALAADLMKIA NDVRWLASGP
310 320 330 340 350
RCGIGEIVIP ENEPGSSIMP GKVNPTQSEA LTMIAAQVMG NDATIGFAAS
360 370 380 390 400
QGNFELNVFK PVIIYNFLQT VQLLSDGMNS FHDKCAVGIE PNQETIQENL
410 420 430 440 450
SNSLMLVTAL NPHIGYENAA KIAKLAHKEG LTLKEAALQL NLLTEEQFNE
460
MVRPEDMVHP KA
Length:462
Mass (Da):50,606
Last modified:May 27, 2015 - v1
Checksum:i85F954B70AF3D86C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP029069 Genomic DNA Translation: AWM45461.1
CP029070 Genomic DNA Translation: AWM49251.1
JZDI01000003 Genomic DNA Translation: KJD58604.1
RefSeqiWP_007410064.1, NZ_MSYT01000002.1

Genome annotation databases

EnsemblBacteriaiKJD58604; KJD58604; UZ38_06110
PATRICifig|1390.149.peg.4128

Similar proteinsi

Entry informationi

Entry nameiA0A0D7XTY8_BACAM
AccessioniPrimary (citable) accession number: A0A0D7XTY8
Entry historyiIntegrated into UniProtKB/TrEMBL: May 27, 2015
Last sequence update: May 27, 2015
Last modified: September 12, 2018
This is version 25 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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