Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Bacillus subtilis
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi108ZincUniRule annotation1
Metal bindingi110ZincUniRule annotation1
Metal bindingi135ZincUniRule annotation1
Metal bindingi137ZincUniRule annotation1
Binding sitei255ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetaseUniRule annotationImported, Ligase
Biological processProtein biosynthesisUniRule annotation
LigandATP-bindingUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding, ZincUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
ORF Names:B4122_1461Imported, BS21228_12795Imported, C7T97_20630Imported, SC09_Contig26orf00030Imported
OrganismiBacillus subtilisImported
Taxonomic identifieri1423 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000239400 Componenti: Chromosome
  • UP000241428 Componenti: Unassembled WGS sequence
  • UP000032247 Componenti: Unassembled WGS sequence
  • UP000076442 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 323tRNA-synt_1cInterPro annotationAdd BLAST320

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi11 – 21"HIGH" regionUniRule annotationAdd BLAST11
Motifi252 – 256"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20 Bacteria
COG0008 LUCA

Family and domain databases

CDDicd00808 GluRS_core, 1 hit
Gene3Di1.10.10.350, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR001412 aa-tRNA-synth_I_CS
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR033910 GluRS_core
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
TIGRFAMsiTIGR00464 gltX_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

A0A0D1IM89-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNEVRVRYA PSPTGHLHIG NARTALFNYL FARNQGGKFI IRVEDTDKKR
60 70 80 90 100
NIEGGEQSQL NYLKWLGIDW DESVDVGGEY GPYRQSERND IYKVYYEELL
110 120 130 140 150
EKGLAYKCYC TEEELEKERE EQIARGEMPR YSGKHRDLTQ EEQEKFIAEG
160 170 180 190 200
RKPSIRFRVP EGKIIAFNDI VKGEISFESD GIGDFVIVKK DGTPTYNFAV
210 220 230 240 250
AIDDYLMKMT HVLRGEDHIS NTPKQIMIYQ AFGWDIPQFG HMTLIVNESR
260 270 280 290 300
KKLSKRDESI IQFIEQYKEL GYLPEALFNF IGLLGWSPVG EEELFTKEQF
310 320 330 340 350
IEIFDVNRLS KSPALFDMHK LKWVNNQYVK KLDLDQVVEL TLPHLQKAGK
360 370 380 390 400
VGTELSAEEQ EWVRKLISLY HEQLSYGAEI VELTDLFFTD EIEYNQEAKA
410 420 430 440 450
VLEEEQVPEV LSTFAAKLEE LEEFTPDNIK ASIKAVQKET GHKGKKLFMP
460 470 480
IRVAVTGQTH GPELPQSIEL IGKETAIQRL KNI
Length:483
Mass (Da):55,736
Last modified:April 29, 2015 - v1
Checksum:i289DB3E8FBA557EB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP020023 Genomic DNA Translation: AVL05191.1
JXBC01000005 Genomic DNA Translation: KIU10258.1
LJZV01000008 Genomic DNA Translation: KZD92986.1
PYBK01000014 Genomic DNA Translation: PSL97055.1
RefSeqiWP_014478617.1, NZ_PYBK01000014.1

Genome annotation databases

EnsemblBacteriaiAOL95893; AOL95893; BS16045_00120
KIU10258; KIU10258; SC09_Contig26orf00030
KZD92986; KZD92986; B4122_1461
PATRICifig|1423.134.peg.1072

Similar proteinsi

Entry informationi

Entry nameiA0A0D1IM89_BACIU
AccessioniPrimary (citable) accession number: A0A0D1IM89
Entry historyiIntegrated into UniProtKB/TrEMBL: April 29, 2015
Last sequence update: April 29, 2015
Last modified: July 18, 2018
This is version 32 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health