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Protein

Pyruvate formate-lyase-activating enzyme

Gene

QU38_14695

Organism
Staphylococcus aureus subsp. aureus
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine.UniRule annotation

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Catalytic activityi

S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

GO - Molecular functioni

Keywordsi

Molecular functionLyaseImported, OxidoreductaseUniRule annotation
Ligand4Fe-4SUniRule annotation, Iron, Iron-sulfur, Metal-binding, PyruvateImported, S-adenosyl-L-methionineUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate formate-lyase-activating enzymeUniRule annotation (EC:1.97.1.4UniRule annotation)
Gene namesi
ORF Names:QU38_14695Imported
OrganismiStaphylococcus aureus subsp. aureusImported
Taxonomic identifieri46170 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000032274 Componenti: Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Structurei

3D structure databases

SMRiA0A0D1EST2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the organic radical-activating enzymes family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105F1A Bacteria
COG1180 LUCA
KOiK04069

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR034457 Organic_radical-activating
IPR012839 Organic_radical_activase
IPR012838 PFL1_activating
IPR034465 Pyruvate_for-lyase_activase
IPR001989 Radical_activat_CS
IPR007197 rSAM
PfamiView protein in Pfam
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF000371 PFL_act_enz, 2 hits
SFLDiSFLDF00278 pyruvate_formate-lyase_activas, 1 hit
SFLDG01066 organic_radical-activating_enz, 1 hit
SFLDS00029 Radical_SAM, 1 hit
TIGRFAMsiTIGR02493 PFLA, 1 hit
PROSITEiView protein in PROSITE
PS01087 RADICAL_ACTIVATING, 1 hit

Sequencei

Sequence statusi: Complete.

A0A0D1EST2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLKGHLHSVE SLGTVDGPGL RYILFTQGCL LRCLYCHNPD TWKISEPSRE
60 70 80 90 100
VTVDEMVNEI LPYKPYFDAS GGGVTVSGGE PLLQMPFLEK LFAELKENGV
110 120 130 140 150
HTCLDTSAGC ANDTKAFQRH FEELQKHTDL ILLDIKHIDN DKHIRLTGKP
160 170 180 190 200
NTHILNFARK LSDMKQPVWI RHVLVPGYSD DKDDLIKLGE FINSLDNVEK
210 220 230 240 250
FEILPYHQLG VHKWKTLGIA YELEDVEAPD DEAVKAAYRY VNFKGKIPVE

L
Length:251
Mass (Da):28,499
Last modified:April 29, 2015 - v1
Checksum:i892A603E273F6C89
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
JXIG01000630 Genomic DNA Translation: KIT95160.1
RefSeqiWP_000911657.1, NZ_PPRK01000035.1

Genome annotation databases

EnsemblBacteriaiKIT95160; KIT95160; QU38_14695
OEH34014; OEH34014; A7U48_3360
KEGGisauf:X998_0202
PATRICifig|46170.102.peg.193

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
JXIG01000630 Genomic DNA Translation: KIT95160.1
RefSeqiWP_000911657.1, NZ_PPRK01000035.1

3D structure databases

SMRiA0A0D1EST2
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiKIT95160; KIT95160; QU38_14695
OEH34014; OEH34014; A7U48_3360
KEGGisauf:X998_0202
PATRICifig|46170.102.peg.193

Phylogenomic databases

eggNOGiENOG4105F1A Bacteria
COG1180 LUCA
KOiK04069

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR034457 Organic_radical-activating
IPR012839 Organic_radical_activase
IPR012838 PFL1_activating
IPR034465 Pyruvate_for-lyase_activase
IPR001989 Radical_activat_CS
IPR007197 rSAM
PfamiView protein in Pfam
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF000371 PFL_act_enz, 2 hits
SFLDiSFLDF00278 pyruvate_formate-lyase_activas, 1 hit
SFLDG01066 organic_radical-activating_enz, 1 hit
SFLDS00029 Radical_SAM, 1 hit
TIGRFAMsiTIGR02493 PFLA, 1 hit
PROSITEiView protein in PROSITE
PS01087 RADICAL_ACTIVATING, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiA0A0D1EST2_STAAU
AccessioniPrimary (citable) accession number: A0A0D1EST2
Entry historyiIntegrated into UniProtKB/TrEMBL: April 29, 2015
Last sequence update: April 29, 2015
Last modified: June 20, 2018
This is version 35 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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