UniProtKB - A0A0B5A051 (PT1L_HUMLU)
2-acylphloroglucinol 4-prenyltransferase
PT1L
Functioni
Involved in the biosynthesis of prenylated phenolics natural products which contribute to the bitter taste of beer and display broad biological activities (Probable). Catalyzes the first prenylation step in the beta-bitter acid pathway (PubMed:25564559).
Uses dimethylallyl diphosphate (DMAPP) as the prenyl donor (PubMed:25564559).
1 Publication1 PublicationCatalytic activityi
- 2',4,4',6'-tetrahydroxychalcone + dimethylallyl diphosphate = desmethylxanthohumol + diphosphate1 PublicationEC:2.5.1.1361 Publication
- a 2-acylphloroglucinol + dimethylallyl diphosphate = a 2-acyl-4-prenylphloroglucinol + diphosphate1 PublicationEC:2.5.1.1361 Publication
Cofactori
Activity regulationi
Kineticsi
- KM=5.74 µM for naringenin chalcone1 Publication
- KM=75.12 µM for dimethylallyl diphosphate1 Publication
- KM=5.01 µM for naringenin chalcone (in the presence of CHIL2)1 Publication
- KM=62.78 µM for dimethylallyl diphosphate (in the presence of CHIL2)1 Publication
- KM=1.36 µM for phlorisovalerophenone1 Publication
- KM=5.1 µM for phlorisobutyrophenone1 Publication
- KM=43.52 µM for dimethylallyl diphosphate1 Publication
- KM=5.7 µM for geranyl diphosphate1 Publication
- Vmax=0.1 µmol/min/g enzyme with naringenin chalcone as substrate1 Publication
- Vmax=0.13 µmol/min/g enzyme with dimethylallyl diphosphate as substrate1 Publication
- Vmax=0.12 µmol/min/g enzyme with naringenin chalcone as substrate (in the presence of CHIL2)1 Publication
- Vmax=0.23 µmol/min/g enzyme with dimethylallyl diphosphate as substrate (in the presence of CHIL2)1 Publication
: Secondary metabolite biosynthesis Pathwayi
This protein is involved in Secondary metabolite biosynthesis.1 PublicationView all proteins of this organism that are known to be involved in Secondary metabolite biosynthesis.
GO - Molecular functioni
- prenyltransferase activity Source: InterPro
Keywordsi
Molecular function | Transferase |
Enzyme and pathway databases
BRENDAi | 2.5.1.136, 2716 |
Names & Taxonomyi
Protein namesi | Recommended name: 2-acylphloroglucinol 4-prenyltransferaseCurated (EC:2.5.1.1362 Publications)Alternative name(s): Aromatic prenyltransferase PT1L1 Publication Humulus lupulus prenyltransferase-1-like1 Publication Short name: HlPT1L1 Publication |
Gene namesi | Name:PT1L1 Publication |
Organismi | Humulus lupulus (European hop) |
Taxonomic identifieri | 3486 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › fabids › Rosales › Cannabaceae › Humulus |
Subcellular locationi
Chloroplast
- chloroplast membrane Sequence analysis; Multi-pass membrane protein Sequence analysis
Other locations
- Plastid 1 Publication
Chloroplast
- chloroplast membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 153 – 173 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 201 – 221 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 229 – 249 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 256 – 276 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 281 – 301 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 336 – 356 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 359 – 379 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 394 – 414 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Chloroplast, Membrane, PlastidPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 183 | D → A: Loss of catalytic activity; when associated with A-187. 1 Publication | 1 | |
Mutagenesisi | 187 | D → A: Loss of catalytic activity; when associated with A-183. 1 Publication | 1 | |
Mutagenesisi | 310 | D → A: Loss of catalytic activity; when associated with A-313. 1 Publication | 1 | |
Mutagenesisi | 313 | D → A: Loss of catalytic activity; when associated with A-310. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 86 | ChloroplastSequence analysisAdd BLAST | 86 | |
ChainiPRO_0000439227 | 87 – 414 | 2-acylphloroglucinol 4-prenyltransferaseSequence analysisAdd BLAST | 328 |
Expressioni
Tissue specificityi
Interactioni
Subunit structurei
Component an active demethylxanthohumol (DMX) biosynthetic metabolon in glandular trichomes (lupulin glands) that encompasses a chalcone synthase (CHS) and a membrane-bound prenyltransferase (PubMed:29760092).
Interacts with PT2, forming a functional metabolon (PubMed:25564559).
Interacts with CHIL2; this interaction promotes catalytic activity (PubMed:29760092).
2 PublicationsStructurei
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transit peptide, Transmembrane, Transmembrane helixFamily and domain databases
CDDi | cd13960, PT_UbiA_HPT1, 1 hit |
Gene3Di | 1.10.357.140, 1 hit |
InterProi | View protein in InterPro IPR044502, AtHST-like IPR000537, UbiA_prenyltransferase IPR044878, UbiA_sf |
Pfami | View protein in Pfam PF01040, UbiA, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MELSSVSSFS LGTNPFISIP HNNNNNLKVS SYCCKSKSRV INSTNSKHCS
60 70 80 90 100
PNNNNNNNTS NKTTHLLGLY GQSRCLLKPL SIFSCKDQRG NSIRASAQIE
110 120 130 140 150
DRPPESGNLS ALTNVKDFVS VCWEYVRPYT AKGVIICSSC LFGRELLENP
160 170 180 190 200
NLFSWPLIFR ALLGMLAILG SCFYTAGINQ IFDMDIDRIN KPDLPLVSGR
210 220 230 240 250
ISVESAWLLT LSPAIIGFIL ILKLNSGPLL TSLYCLAILS GTIYSVPPFR
260 270 280 290 300
WKKNPITAFL CILMIHAGLN FSVYYASRAA LGLAFVWSPS FSFITAFITF
310 320 330 340 350
MTLTLASSKD LSDINGDRKF GVETFATKLG AKNITLLGTG LLLLNYVAAI
360 370 380 390 400
STAIIWPKAF KSNIMLLSHA ILAFSLFFQA RELDRTNYTP EACKSFYEFI
410
WILFSAEYVV YLFI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | KM222441 mRNA Translation: AJD80254.1 |
Genome annotation databases
KEGGi | ag:AJD80254 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | KM222441 mRNA Translation: AJD80254.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Genome annotation databases
KEGGi | ag:AJD80254 |
Enzyme and pathway databases
BRENDAi | 2.5.1.136, 2716 |
Family and domain databases
CDDi | cd13960, PT_UbiA_HPT1, 1 hit |
Gene3Di | 1.10.357.140, 1 hit |
InterProi | View protein in InterPro IPR044502, AtHST-like IPR000537, UbiA_prenyltransferase IPR044878, UbiA_sf |
Pfami | View protein in Pfam PF01040, UbiA, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PT1L_HUMLU | |
Accessioni | A0A0B5A051Primary (citable) accession number: A0A0B5A051 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 15, 2017 |
Last sequence update: | April 1, 2015 | |
Last modified: | February 23, 2022 | |
This is version 18 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Documents
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families