UniProtKB - A0A0B4J1G0 (FCG3A_MOUSE)
Low affinity immunoglobulin gamma Fc region receptor III-A
Fcgr4
Functioni
Receptor for the invariable Fc fragment of immunoglobulin gamma (IgG) (PubMed:16039578).
Binds with intermediate affinity to both IgG2a and IgG2b (PubMed:16039578, PubMed:17558411, PubMed:19795417).
Can bind to IgG2a and IgG2b monomers (PubMed:18949059).
Does not display binding to IgG1 or IgG3 (PubMed:16039578).
Recognizes neutralizing virus-specific IgGs displayed on the cell surface of infected cells and triggers antibody-dependent cellular cytotoxicity (ADCC). Confers protection to lethal influenza virus infection (PubMed:24412922).
On splenic dendritic cells, uptakes antigen immune complexes and efficiently divert them into MHC class I and II antigen presentation pathways to provide for superior priming of CD4-positive and CD8-positive T cell immune responses (PubMed:28389502).
Mediates neutrophil activation by IgG complexes redundantly with FCGR2A (PubMed:18097064).
Plays a role in promoting bone resorption by enhancing osteoclast differentiation following binding to IgG2a (PubMed:25824719).
Also acts as a receptor for the Fc region of immunoglobulin epsilon (IgE) (PubMed:17558411, PubMed:18949059).
Binds with low affinity to both the a and b allotypes of IgE (PubMed:18949059).
Has also been shown to bind to IgE allotype a only but not to allotype b (PubMed:17558411).
Binds aggregated IgE but not the monomeric form and bound monomeric IgG is readily displaced by IgE complexes (PubMed:18949059).
Binding to IgE promotes macrophage-mediated phagocytosis, antigen presentation to T cells, production of pro-inflammatory cytokines and the late phase of cutaneous allergic reactions (PubMed:17558411, PubMed:18949059).
Mediates enhanced ADCC in response to afucosylated IgGs (PubMed:34485821).
9 PublicationsSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 217 | Important for receptor turnoverBy similarity | 1 |
GO - Molecular functioni
- GPI anchor binding Source: MGI
- IgE binding Source: UniProtKB-KW
- IgE receptor activity Source: UniProtKB
- IgG binding Source: UniProtKB-KW
- IgG receptor activity Source: UniProtKB
- immune receptor activity Source: MGI
- low-affinity IgG receptor activity Source: MGI
- transmembrane signaling receptor activity Source: GO_Central
GO - Biological processi
- cell surface receptor signaling pathway Source: GO_Central
- cellular response to lipopolysaccharide Source: MGI
- dendritic cell antigen processing and presentation Source: UniProtKB
- Fc receptor-mediated immune complex endocytosis Source: UniProtKB
- neutrophil activation Source: UniProtKB
- positive regulation of bone resorption Source: UniProtKB
- regulation of immune response Source: GO_Central
- regulation of sensory perception of pain Source: MGI
Keywordsi
Molecular function | IgE-binding protein, IgG-binding protein, Receptor |
Biological process | Immunity |
Enzyme and pathway databases
Reactomei | R-MMU-198933, Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell R-MMU-2029481, FCGR activation R-MMU-2029482, Regulation of actin dynamics for phagocytic cup formation R-MMU-2029485, Role of phospholipids in phagocytosis |
Names & Taxonomyi
Protein namesi | Recommended name: Low affinity immunoglobulin gamma Fc region receptor III-ABy similarityShort name: IgG Fc receptor III-A Alternative name(s): CD16-21 Publication FcgammaRIV1 Publication CD_antigen: CD16a |
Gene namesi | |
Organismi | Mus musculus (Mouse)Imported |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2179523, Fcgr4 |
VEuPathDBi | HostDB:ENSMUSG00000059089 |
Subcellular locationi
Plasma membrane
- Cell membrane 3 Publications; Single-pass type I membrane protein Sequence analysis
Extracellular region or secreted
- extracellular space Source: MGI
Plasma Membrane
- external side of plasma membrane Source: MGI
- Fc-gamma receptor III complex Source: MGI
- integral component of plasma membrane Source: GO_Central
- plasma membrane Source: UniProtKB
Other locations
- cell surface Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 21 – 203 | ExtracellularCuratedAdd BLAST | 183 | |
Transmembranei | 204 – 224 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 225 – 249 | CytoplasmicCuratedAdd BLAST | 25 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 235 | Y → W: Abolishes receptor ligation-induced phosphorylation. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 20 | Sequence analysisAdd BLAST | 20 | |
ChainiPRO_5008204824 | 21 – 249 | Low affinity immunoglobulin gamma Fc region receptor III-ASequence analysisAdd BLAST | 229 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 46 ↔ 88 | PROSITE-ProRule annotation | ||
Glycosylationi | 62 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 | |
Disulfide bondi | 127 ↔ 171 | PROSITE-ProRule annotation | ||
Glycosylationi | 164 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 | |
Glycosylationi | 179 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 | |
Modified residuei | 235 | PhosphotyrosineCombined sources1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
PaxDbi | A0A0B4J1G0 |
ProteomicsDBi | 271732 |
PTM databases
GlyGeni | A0A0B4J1G0, 3 sites |
iPTMneti | A0A0B4J1G0 |
PhosphoSitePlusi | A0A0B4J1G0 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSMUSG00000059089, Expressed in granulocyte and 96 other tissues |
Interactioni
Subunit structurei
Forms a heterooligomeric complex with ITAM-containing signaling subunits FCER1G.
Interacts (via transmembrane domain) with signaling subunits; this interaction is a prerequisite for receptor complex expression on the cell surface and intracellular signal transduction. Binds the Fc region of antigen-complexed IgG.
2 PublicationsProtein-protein interaction databases
STRINGi | 10090.ENSMUSP00000077873 |
Miscellaneous databases
RNActi | A0A0B4J1G0, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 22 – 102 | Ig-like C2-type 1PROSITE-ProRule annotationAdd BLAST | 81 | |
Domaini | 119 – 188 | Ig-like C2-type 2PROSITE-ProRule annotationAdd BLAST | 70 |
Keywords - Domaini
Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502RU1M, Eukaryota |
GeneTreei | ENSGT01040000240489 |
HOGENOMi | CLU_023383_1_0_1 |
OMAi | GDNSTQW |
OrthoDBi | 866496at2759 |
PhylomeDBi | A0A0B4J1G0 |
Family and domain databases
Gene3Di | 2.60.40.10, 2 hits |
InterProi | View protein in InterPro IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR003599, Ig_sub IPR003598, Ig_sub2 |
Pfami | View protein in Pfam PF13895, Ig_2, 2 hits |
SMARTi | View protein in SMART SM00409, IG, 2 hits SM00408, IGc2, 2 hits |
SUPFAMi | SSF48726, SSF48726, 2 hits |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 2 hits |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MWQLLLPTAL VLTAFSGIQA GLQKAVVNLD PKWVRVLEED SVTLRCQGTF
60 70 80 90 100
SPEDNSIKWF HNESLIPHQD ANYVIQSARV KDSGMYRCQT ALSTISDPVQ
110 120 130 140 150
LEVHMGWLLL QTTKWLFQEG DPIHLRCHSW QNRPVRKVTY LQNGKGKKYF
160 170 180 190 200
HENSELLIPK ATHNDSGSYF CRGLIGHNNK SSASFRISLG DPGSPSMFPP
210 220 230 240
WHQITFCLLI GLLFAIDTVL YFSVRRGLQS PVADYEEPKI QWSKEPQDK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 57 | I → T in ABS89131 (PubMed:18292508).Curated | 1 | |
Sequence conflicti | 57 | I → T in BAE42113 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 141 | L → S in AAH27310 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 157 | L → P in AAM19249 (PubMed:12389094).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF499613 mRNA Translation: AAM19249.1 EU050648 mRNA Translation: ABS89131.1 AK170920 mRNA Translation: BAE42113.1 AC115959 Genomic DNA No translation available. CH466520 Genomic DNA Translation: EDL39140.1 BC027310 mRNA Translation: AAH27310.1 |
CCDSi | CCDS15476.1 |
RefSeqi | NP_653142.2, NM_144559.2 |
Genome annotation databases
Ensembli | ENSMUST00000078825; ENSMUSP00000077873; ENSMUSG00000059089 |
GeneIDi | 246256 |
KEGGi | mmu:246256 |
UCSCi | uc007dmw.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF499613 mRNA Translation: AAM19249.1 EU050648 mRNA Translation: ABS89131.1 AK170920 mRNA Translation: BAE42113.1 AC115959 Genomic DNA No translation available. CH466520 Genomic DNA Translation: EDL39140.1 BC027310 mRNA Translation: AAH27310.1 |
CCDSi | CCDS15476.1 |
RefSeqi | NP_653142.2, NM_144559.2 |
3D structure databases
AlphaFoldDBi | A0A0B4J1G0 |
SMRi | A0A0B4J1G0 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000077873 |
PTM databases
GlyGeni | A0A0B4J1G0, 3 sites |
iPTMneti | A0A0B4J1G0 |
PhosphoSitePlusi | A0A0B4J1G0 |
Proteomic databases
PaxDbi | A0A0B4J1G0 |
ProteomicsDBi | 271732 |
Protocols and materials databases
DNASUi | 246256 |
Genome annotation databases
Ensembli | ENSMUST00000078825; ENSMUSP00000077873; ENSMUSG00000059089 |
GeneIDi | 246256 |
KEGGi | mmu:246256 |
UCSCi | uc007dmw.2, mouse |
Organism-specific databases
CTDi | 246256 |
MGIi | MGI:2179523, Fcgr4 |
VEuPathDBi | HostDB:ENSMUSG00000059089 |
Phylogenomic databases
eggNOGi | ENOG502RU1M, Eukaryota |
GeneTreei | ENSGT01040000240489 |
HOGENOMi | CLU_023383_1_0_1 |
OMAi | GDNSTQW |
OrthoDBi | 866496at2759 |
PhylomeDBi | A0A0B4J1G0 |
Enzyme and pathway databases
Reactomei | R-MMU-198933, Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell R-MMU-2029481, FCGR activation R-MMU-2029482, Regulation of actin dynamics for phagocytic cup formation R-MMU-2029485, Role of phospholipids in phagocytosis |
Miscellaneous databases
BioGRID-ORCSi | 246256, 1 hit in 75 CRISPR screens |
PROi | PR:A0A0B4J1G0 |
RNActi | A0A0B4J1G0, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000059089, Expressed in granulocyte and 96 other tissues |
Family and domain databases
Gene3Di | 2.60.40.10, 2 hits |
InterProi | View protein in InterPro IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR003599, Ig_sub IPR003598, Ig_sub2 |
Pfami | View protein in Pfam PF13895, Ig_2, 2 hits |
SMARTi | View protein in SMART SM00409, IG, 2 hits SM00408, IGc2, 2 hits |
SUPFAMi | SSF48726, SSF48726, 2 hits |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | FCG3A_MOUSE | |
Accessioni | A0A0B4J1G0Primary (citable) accession number: A0A0B4J1G0 Secondary accession number(s): Q3TC44, Q8R2R4, Q8R477 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 18, 2017 |
Last sequence update: | March 4, 2015 | |
Last modified: | May 25, 2022 | |
This is version 52 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot