UniProtKB - A0A0B4J1G0 (FCGR4_MOUSE)
Protein
Low affinity immunoglobulin gamma Fc region receptor IV
Gene
Fcgr4
Organism
Mus musculus (Mouse)
Status
Functioni
Receptor for the Fc region of immunoglobulin gamma (PubMed:16039578). Also acts as a receptor for the Fc region of immunoglobulin epsilon (PubMed:17558411, PubMed:18949059). Binds with intermediate affinity to both IgG2a and IgG2b (PubMed:16039578, PubMed:17558411, PubMed:19795417). Can bind to IgG2a and IgG2b monomers (PubMed:18949059). Does not display binding to IgG1 or IgG3 (PubMed:16039578). Mediates neutrophil activation by IgG complexes redundantly with Fcgr3 (PubMed:18097064). Plays a role in promoting bone resorption by enhancing osteoclast differentiation following binding to IgG2a (PubMed:25824719). Binds with low affinity to both the a and b allotypes of IgE (PubMed:18949059). Has also been shown to bind to IgE allotype a only but not to allotype b (PubMed:17558411). Binds aggregated IgE but not the monomeric form and bound monomeric IgG is readily displaced by IgE complexes (PubMed:18949059). Binding to IgE promotes macrophage-mediated phagocytosis, antigen presentation to T cells, production of proinflammatory cytokines and the late phase of cutaneous allergic reactions (PubMed:17558411, PubMed:18949059).6 Publications
GO - Molecular functioni
- IgE binding Source: UniProtKB-KW
- IgE receptor activity Source: UniProtKB
- IgG binding Source: UniProtKB-KW
- IgG receptor activity Source: UniProtKB
- transmembrane signaling receptor activity Source: GO_Central
GO - Biological processi
- cell surface receptor signaling pathway Source: GO_Central
- cellular response to lipopolysaccharide Source: MGI
- neutrophil activation Source: UniProtKB
- positive regulation of bone resorption Source: UniProtKB
- regulation of immune response Source: GO_Central
- regulation of sensory perception of pain Source: MGI
- wound healing Source: Ensembl
Keywordsi
Molecular function | IgE-binding protein, IgG-binding protein, Receptor |
Enzyme and pathway databases
Reactomei | R-MMU-163125, Post-translational modification: synthesis of GPI-anchored proteins R-MMU-198933, Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell R-MMU-2029481, FCGR activation R-MMU-2029482, Regulation of actin dynamics for phagocytic cup formation R-MMU-2029485, Role of phospholipids in phagocytosis R-MMU-6798695, Neutrophil degranulation |
Names & Taxonomyi
Protein namesi | Recommended name: Low affinity immunoglobulin gamma Fc region receptor IVImportedAlternative name(s): CD16-21 Publication FcgammaRIV1 Publication |
Gene namesi | Name:Fcgr4Imported |
Organismi | Mus musculus (Mouse)Imported |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2179523, Fcgr4 |
Subcellular locationi
Plasma membrane
- Cell membrane 2 Publications; Single-pass type I membrane protein Sequence analysis
Note: Cell surface expression is dependent on the presence of the Fc receptor gamma chain Fcerg.2 Publications
Plasma Membrane
- external side of plasma membrane Source: MGI
- integral component of plasma membrane Source: GO_Central
Other locations
- cell surface Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 21 – 203 | ExtracellularCuratedAdd BLAST | 183 | |
Transmembranei | 204 – 224 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 225 – 249 | CytoplasmicCuratedAdd BLAST | 25 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 235 | Y → W: Abolishes receptor ligation-induced phosphorylation. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 20 | Sequence analysisAdd BLAST | 20 | |
ChainiPRO_5008204824 | 21 – 249 | Low affinity immunoglobulin gamma Fc region receptor IVSequence analysisAdd BLAST | 229 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 46 ↔ 88 | PROSITE-ProRule annotation | ||
Glycosylationi | 62 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 | |
Disulfide bondi | 127 ↔ 171 | PROSITE-ProRule annotation | ||
Glycosylationi | 164 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 | |
Glycosylationi | 179 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 | |
Modified residuei | 235 | PhosphotyrosineCombined sources1 Publication | 1 |
Post-translational modificationi
N-glycosylated.2 Publications
Phosphorylated following receptor ligation.1 Publication
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
PaxDbi | A0A0B4J1G0 |
PTM databases
GlyGeni | A0A0B4J1G0, 3 sites |
iPTMneti | A0A0B4J1G0 |
PhosphoSitePlusi | A0A0B4J1G0 |
Expressioni
Tissue specificityi
Detected on myeloid cells, peripheral blood monocytes, splenic and bone marrow dendritic cells, and thioglycollate-elicited macrophages and neutrophils but absent from lymphoid populations with no expression observed on T cells, B cells, NK cells or other granulocytes (at protein level) (PubMed:16039578, PubMed:19795417). Expressed in peripheral blood leukocytes, spleen, liver, thymus and small intestine (PubMed:12389094, PubMed:17558411).4 Publications
Inductioni
By lipopolysaccharide, interferon beta and IFNG.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000059089, Expressed in granulocyte and 86 other tissues |
Interactioni
Subunit structurei
Interacts with the Fc receptor gamma chain Fcerg; Fcerg is required for the cell surface expression of Fcrg4.
2 PublicationsProtein-protein interaction databases
STRINGi | 10090.ENSMUSP00000077873 |
Miscellaneous databases
RNActi | A0A0B4J1G0, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 22 – 102 | Ig-like C2-type 1PROSITE-ProRule annotationAdd BLAST | 81 | |
Domaini | 119 – 188 | Ig-like C2-type 2PROSITE-ProRule annotationAdd BLAST | 70 |
Keywords - Domaini
Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502RU1M, Eukaryota |
GeneTreei | ENSGT01000000214398 |
HOGENOMi | CLU_023383_1_0_1 |
OMAi | GDNSTQW |
OrthoDBi | 866496at2759 |
PhylomeDBi | A0A0B4J1G0 |
Family and domain databases
Gene3Di | 2.60.40.10, 2 hits |
InterProi | View protein in InterPro IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR003599, Ig_sub IPR003598, Ig_sub2 |
Pfami | View protein in Pfam PF13895, Ig_2, 1 hit |
SMARTi | View protein in SMART SM00409, IG, 2 hits SM00408, IGc2, 2 hits |
SUPFAMi | SSF48726, SSF48726, 2 hits |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 2 hits |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
A0A0B4J1G0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MWQLLLPTAL VLTAFSGIQA GLQKAVVNLD PKWVRVLEED SVTLRCQGTF
60 70 80 90 100
SPEDNSIKWF HNESLIPHQD ANYVIQSARV KDSGMYRCQT ALSTISDPVQ
110 120 130 140 150
LEVHMGWLLL QTTKWLFQEG DPIHLRCHSW QNRPVRKVTY LQNGKGKKYF
160 170 180 190 200
HENSELLIPK ATHNDSGSYF CRGLIGHNNK SSASFRISLG DPGSPSMFPP
210 220 230 240
WHQITFCLLI GLLFAIDTVL YFSVRRGLQS PVADYEEPKI QWSKEPQDK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 57 | I → T in ABS89131 (PubMed:18292508).Curated | 1 | |
Sequence conflicti | 57 | I → T in BAE42113 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 141 | L → S in AAH27310 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 157 | L → P in AAM19249 (PubMed:12389094).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF499613 mRNA Translation: AAM19249.1 EU050648 mRNA Translation: ABS89131.1 AK170920 mRNA Translation: BAE42113.1 AC115959 Genomic DNA No translation available. CH466520 Genomic DNA Translation: EDL39140.1 BC027310 mRNA Translation: AAH27310.1 |
CCDSi | CCDS15476.1 |
RefSeqi | NP_653142.2, NM_144559.2 |
Genome annotation databases
Ensembli | ENSMUST00000078825; ENSMUSP00000077873; ENSMUSG00000059089 |
GeneIDi | 246256 |
KEGGi | mmu:246256 |
UCSCi | uc007dmw.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF499613 mRNA Translation: AAM19249.1 EU050648 mRNA Translation: ABS89131.1 AK170920 mRNA Translation: BAE42113.1 AC115959 Genomic DNA No translation available. CH466520 Genomic DNA Translation: EDL39140.1 BC027310 mRNA Translation: AAH27310.1 |
CCDSi | CCDS15476.1 |
RefSeqi | NP_653142.2, NM_144559.2 |
3D structure databases
SMRi | A0A0B4J1G0 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000077873 |
PTM databases
GlyGeni | A0A0B4J1G0, 3 sites |
iPTMneti | A0A0B4J1G0 |
PhosphoSitePlusi | A0A0B4J1G0 |
Proteomic databases
PaxDbi | A0A0B4J1G0 |
Genome annotation databases
Ensembli | ENSMUST00000078825; ENSMUSP00000077873; ENSMUSG00000059089 |
GeneIDi | 246256 |
KEGGi | mmu:246256 |
UCSCi | uc007dmw.2, mouse |
Organism-specific databases
CTDi | 246256 |
MGIi | MGI:2179523, Fcgr4 |
Phylogenomic databases
eggNOGi | ENOG502RU1M, Eukaryota |
GeneTreei | ENSGT01000000214398 |
HOGENOMi | CLU_023383_1_0_1 |
OMAi | GDNSTQW |
OrthoDBi | 866496at2759 |
PhylomeDBi | A0A0B4J1G0 |
Enzyme and pathway databases
Reactomei | R-MMU-163125, Post-translational modification: synthesis of GPI-anchored proteins R-MMU-198933, Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell R-MMU-2029481, FCGR activation R-MMU-2029482, Regulation of actin dynamics for phagocytic cup formation R-MMU-2029485, Role of phospholipids in phagocytosis R-MMU-6798695, Neutrophil degranulation |
Miscellaneous databases
BioGRID-ORCSi | 246256, 0 hits in 17 CRISPR screens |
PROi | PR:A0A0B4J1G0 |
RNActi | A0A0B4J1G0, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000059089, Expressed in granulocyte and 86 other tissues |
Family and domain databases
Gene3Di | 2.60.40.10, 2 hits |
InterProi | View protein in InterPro IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR003599, Ig_sub IPR003598, Ig_sub2 |
Pfami | View protein in Pfam PF13895, Ig_2, 1 hit |
SMARTi | View protein in SMART SM00409, IG, 2 hits SM00408, IGc2, 2 hits |
SUPFAMi | SSF48726, SSF48726, 2 hits |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FCGR4_MOUSE | |
Accessioni | A0A0B4J1G0Primary (citable) accession number: A0A0B4J1G0 Secondary accession number(s): Q3TC44, Q8R2R4, Q8R477 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 18, 2017 |
Last sequence update: | March 4, 2015 | |
Last modified: | December 2, 2020 | |
This is version 47 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot