Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Diaminopimelate epimerase

Gene

dapF

Organism
Campylobacter lari RM16712
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Catalytic activityi

LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotationSAAS annotation

Pathwayi: L-lysine biosynthesis via DAP pathway

This protein is involved in step 1 of the subpathway that synthesizes DL-2,6-diaminopimelate from LL-2,6-diaminopimelate.UniRule annotationSAAS annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Diaminopimelate epimerase (dapF)
This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes DL-2,6-diaminopimelate from LL-2,6-diaminopimelate, the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei11SubstrateUniRule annotation1
Binding sitei60SubstrateUniRule annotation1
Active sitei69PROSITE-ProRule annotation1
Active sitei69Proton donorUniRule annotation1
Sitei138Could be important to modulate the pK values of the two catalytic cysteine residuesUniRule annotation1
Binding sitei164SubstrateUniRule annotation1
Sitei182Could be important to modulate the pK values of the two catalytic cysteine residuesUniRule annotation1
Active sitei192Proton acceptorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomeraseUniRule annotationSAAS annotationImported
Biological processAmino-acid biosynthesis, Lysine biosynthesisUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayi
UPA00034;UER00025

Names & Taxonomyi

Protein namesi
Recommended name:
Diaminopimelate epimeraseUniRule annotationSAAS annotation (EC:5.1.1.7UniRule annotationSAAS annotation)
Short name:
DAP epimeraseUniRule annotation
Alternative name(s):
PLP-independent amino acid racemaseUniRule annotation
Gene namesi
Name:dapFUniRule annotationImported
ORF Names:UPTC16712_0065Imported
OrganismiCampylobacter lari RM16712Imported
Taxonomic identifieri1453988 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
Proteomesi
  • UP000031123 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni70 – 71Substrate bindingUniRule annotation2
Regioni182 – 183Substrate bindingUniRule annotation2
Regioni193 – 194Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the diaminopimelate epimerase family.UniRule annotationSAAS annotation

Phylogenomic databases

KOiK01778

Family and domain databases

HAMAPiMF_00197 DAP_epimerase, 1 hit
InterProiView protein in InterPro
IPR018510 DAP_epimerase_AS
IPR001653 DAP_epimerase_DapF
PANTHERiPTHR31689 PTHR31689, 1 hit
PfamiView protein in Pfam
PF01678 DAP_epimerase, 2 hits
TIGRFAMsiTIGR00652 DapF, 1 hit
PROSITEiView protein in PROSITE
PS01326 DAP_EPIMERASE, 1 hit

Sequencei

Sequence statusi: Complete.

A0A0A8I5G1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKFYKYCASG NDFVVFADSE KRDRSELAKI LCNRYEGIGA DGLIVVVPHD
60 70 80 90 100
RYDFEWEFYN CDGSKASMCG NGSRAAAHFV HHYLKKSQYL NFLTGAGLIK
110 120 130 140 150
SFVDDDIVEI KLSGVKDIKE AFEYKGRIWQ GCNTGVPHIV TFVDDLNEFD
160 170 180 190 200
VNLCKEVRKK YNANVNYAKV EDDEFIRVRT YERGVEDETL ACGTGMGACF
210 220 230 240
YLAYLNQKVK DDILVKPKSN ESLYFRLEED QIFFRGKVKC CFEADYNFT
Length:249
Mass (Da):28,643
Last modified:March 4, 2015 - v1
Checksum:iD86A52D48514200A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP007778 Genomic DNA Translation: AJD05614.1
RefSeqiWP_039640490.1, NZ_CP007778.1

Genome annotation databases

EnsemblBacteriaiAJD05614; AJD05614; UPTC16712_0065
KEGGiclm:UPTC16712_0065

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP007778 Genomic DNA Translation: AJD05614.1
RefSeqiWP_039640490.1, NZ_CP007778.1

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAJD05614; AJD05614; UPTC16712_0065
KEGGiclm:UPTC16712_0065

Phylogenomic databases

KOiK01778

Enzyme and pathway databases

UniPathwayi
UPA00034;UER00025

Family and domain databases

HAMAPiMF_00197 DAP_epimerase, 1 hit
InterProiView protein in InterPro
IPR018510 DAP_epimerase_AS
IPR001653 DAP_epimerase_DapF
PANTHERiPTHR31689 PTHR31689, 1 hit
PfamiView protein in Pfam
PF01678 DAP_epimerase, 2 hits
TIGRFAMsiTIGR00652 DapF, 1 hit
PROSITEiView protein in PROSITE
PS01326 DAP_EPIMERASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiA0A0A8I5G1_CAMLA
AccessioniPrimary (citable) accession number: A0A0A8I5G1
Entry historyiIntegrated into UniProtKB/TrEMBL: March 4, 2015
Last sequence update: March 4, 2015
Last modified: September 27, 2017
This is version 20 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again