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Protein

Fumarate hydratase class II

Gene

fumC

Organism
Bacillus subtilis
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.UniRule annotation

Miscellaneous

There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.UniRule annotation

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Catalytic activityi

(S)-malate = fumarate + H2O.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from fumarate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from fumarate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei185SubstrateUniRule annotation1
Active sitei186Proton donor/acceptorUniRule annotation1
Active sitei316UniRule annotation1
Binding sitei317SubstrateUniRule annotation1
Sitei329Important for catalytic activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseUniRule annotationSAAS annotation
Biological processTricarboxylic acid cycleUniRule annotation

Enzyme and pathway databases

UniPathwayi
UPA00223;UER01007

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate hydratase class IIUniRule annotation (EC:4.2.1.2UniRule annotation)
Short name:
Fumarase CUniRule annotation
Alternative name(s):
Aerobic fumaraseUniRule annotation
Iron-independent fumaraseUniRule annotation
Gene namesi
Name:fumCUniRule annotationImported
ORF Names:B4122_3151Imported, B4417_3118Imported, DLD52_07755Imported, SC09_contig4orf00374Imported
OrganismiBacillus subtilisImported
Taxonomic identifieri1423 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000076589 Componenti: Unassembled WGS sequence
  • UP000032247 Componenti: Unassembled WGS sequence
  • UP000076442 Componenti: Unassembled WGS sequence
  • UP000245387 Componenti: Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 340Lyase_1InterPro annotationAdd BLAST330
Domaini406 – 458FumaraseC_CInterPro annotationAdd BLAST53

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni97 – 99Substrate bindingUniRule annotation3
Regioni127 – 130Substrate binding (B site)UniRule annotation4
Regioni137 – 139Substrate bindingUniRule annotation3
Regioni322 – 324Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C9Q Bacteria
COG0114 LUCA

Family and domain databases

CDDicd01362 Fumarase_classII, 1 hit
Gene3Di1.10.275.10, 1 hit
HAMAPiMF_00743 FumaraseC, 1 hit
InterProiView protein in InterPro
IPR005677 Fum_hydII
IPR024083 Fumarase/histidase_N
IPR018951 Fumarase_C_C
IPR020557 Fumarate_lyase_CS
IPR000362 Fumarate_lyase_fam
IPR022761 Fumarate_lyase_N
IPR008948 L-Aspartase-like
PANTHERiPTHR11444 PTHR11444, 1 hit
PfamiView protein in Pfam
PF10415 FumaraseC_C, 1 hit
PF00206 Lyase_1, 1 hit
PRINTSiPR00149 FUMRATELYASE
SUPFAMiSSF48557 SSF48557, 1 hit
TIGRFAMsiTIGR00979 fumC_II, 1 hit
PROSITEiView protein in PROSITE
PS00163 FUMARATE_LYASES, 1 hit

Sequencei

Sequence statusi: Complete.

A0A0A1M8M3-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEYRIERDTM GEVKVPADKF WGAQTQRSKE NFKIGSEKMP MRVVKAFAIL
60 70 80 90 100
KRSTALANKR LGNLDAEKAE AIAAVCDDVL KGKYDDNFPL VVWQTGSGTQ
110 120 130 140 150
SNMNMNEVVA NRATALLKEK NSDQTIHPND DVNRSQSSND TFPTAMHVAA
160 170 180 190 200
VLAVYEQLVP ALDQLRNTLD EKAKAYNDIV KIGRTHLQDA TPLTLGQEIS
210 220 230 240 250
GWVHMLDRSK EMILEATDKM RALAIGGTAV GTGINAHPEF GELVSEEITK
260 270 280 290 300
LTGQTFSSSP NKFHALTSHD EITYAHGALK ALAADLMKIA NDVRWLASGP
310 320 330 340 350
RCGIGEIVIP ENEPGSSIMP GKVNPTQSEA LTMIAAQIMG NDATIGFAAS
360 370 380 390 400
QGNFELNVFK PVIIYNFLQS VQLLSDGMNS FHDKCAVGIE PNKETIQENL
410 420 430 440 450
SNSLMLVTAL NPHIGYENAA KIAKLAHKEG LTLKEAALKL ELLTEEQFNE
460
MVKPEDMVKP KA
Length:462
Mass (Da):50,532
Last modified:February 4, 2015 - v1
Checksum:i8D797D18FEA82C06
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
JXBC01000013 Genomic DNA Translation: KIU05553.1
LJSM01000042 Genomic DNA Translation: KZD78329.1
LJZV01000016 Genomic DNA Translation: KZD90230.1
QGQX01000002 Genomic DNA Translation: PWT19725.1
RefSeqiWP_003228523.1, NZ_PISO01000015.1

Genome annotation databases

EnsemblBacteriaiAOL99162; AOL99162; BS16045_03472
KIU05553; KIU05553; SC09_contig4orf00374
KZD78329; KZD78329; B4417_3118
KZD90230; KZD90230; B4122_3151
PATRICifig|1423.134.peg.2037

Similar proteinsi

Entry informationi

Entry nameiA0A0A1M8M3_BACIU
AccessioniPrimary (citable) accession number: A0A0A1M8M3
Entry historyiIntegrated into UniProtKB/TrEMBL: February 4, 2015
Last sequence update: February 4, 2015
Last modified: September 12, 2018
This is version 39 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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