UniProtKB - A0A099MV40 (A0A099MV40_9PSED)
Protein
Isoleucine--tRNA ligase
Gene
ileS
Organism
Pseudomonas plecoglossicida
Status
Functioni
Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation
Catalytic activityi
- ATP + L-isoleucine + tRNAIle = AMP + diphosphate + L-isoleucyl-tRNAIleUniRule annotationARBA annotationEC:6.1.1.5UniRule annotationARBA annotation
Cofactori
Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 567 | Aminoacyl-adenylateUniRule annotation | 1 | |
Binding sitei | 611 | ATPUniRule annotation | 1 | |
Metal bindingi | 906 | ZincUniRule annotation | 1 | |
Metal bindingi | 909 | ZincUniRule annotation | 1 | |
Metal bindingi | 926 | ZincUniRule annotation | 1 | |
Metal bindingi | 929 | ZincUniRule annotation | 1 |
GO - Molecular functioni
- aminoacyl-tRNA editing activity Source: InterPro
- ATP binding Source: UniProtKB-UniRule
- isoleucine-tRNA ligase activity Source: UniProtKB-UniRule
- tRNA binding Source: InterPro
- zinc ion binding Source: UniProtKB-UniRule
GO - Biological processi
- isoleucyl-tRNA aminoacylation Source: UniProtKB-UniRule
Keywordsi
Molecular function | Aminoacyl-tRNA synthetaseUniRule annotationImportedARBA annotation, Ligase |
Biological process | Protein biosynthesisUniRule annotationARBA annotation |
Ligand | ATP-bindingUniRule annotationARBA annotation, Metal-bindingUniRule annotationARBA annotation, Nucleotide-binding, ZincUniRule annotation |
Names & Taxonomyi
Protein namesi | Recommended name: Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)Alternative name(s): Isoleucyl-tRNA synthetaseUniRule annotation Short name: IleRSUniRule annotation |
Gene namesi | Name:ileSUniRule annotation ORF Names:CMV24_27595Imported, RK21_04398Imported |
Organismi | Pseudomonas plecoglossicidaImported |
Taxonomic identifieri | 70775 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmUniRule annotationARBA annotationInteractioni
Subunit structurei
Monomer.
UniRule annotationFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 27 – 646 | tRNA-synt_1InterPro annotationAdd BLAST | 620 | |
Domaini | 691 – 847 | Anticodon_1InterPro annotationAdd BLAST | 157 | |
Domaini | 905 – 931 | zf-FPG_IleRSInterPro annotationAdd BLAST | 27 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 58 – 68 | 'HIGH' regionUniRule annotationAdd BLAST | 11 | |
Motifi | 608 – 612 | 'KMSKS' regionUniRule annotation | 5 |
Domaini
IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation
Sequence similaritiesi
Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotationARBA annotation
Family and domain databases
CDDi | cd07960, Anticodon_Ia_Ile_BEm, 1 hit |
Gene3Di | 3.40.50.620, 1 hit 3.90.740.10, 1 hit |
HAMAPi | MF_02002, Ile_tRNA_synth_type1, 1 hit |
InterProi | View protein in InterPro IPR001412, aa-tRNA-synth_I_CS IPR002300, aa-tRNA-synth_Ia IPR033708, Anticodon_Ile_BEm IPR002301, Ile-tRNA-ligase IPR023585, Ile-tRNA-ligase_type1 IPR013155, M/V/L/I-tRNA-synth_anticd-bd IPR014729, Rossmann-like_a/b/a_fold IPR009080, tRNAsynth_Ia_anticodon-bd IPR009008, Val/Leu/Ile-tRNA-synth_edit IPR010663, Znf_FPG/IleRS |
Pfami | View protein in Pfam PF08264, Anticodon_1, 1 hit PF00133, tRNA-synt_1, 1 hit PF06827, zf-FPG_IleRS, 1 hit |
PRINTSi | PR00984, TRNASYNTHILE |
SUPFAMi | SSF47323, SSF47323, 1 hit SSF50677, SSF50677, 1 hit |
TIGRFAMsi | TIGR00392, ileS, 1 hit |
PROSITEi | View protein in PROSITE PS00178, AA_TRNA_LIGASE_I, 1 hit |
i Sequence
Sequence statusi: Complete.
A0A099MV40-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTDYKATLNL PDTAFPMKAG LPQREPQILQ RWDSIGLYQK LREIGKDRPK
60 70 80 90 100
FVLHDGPPYA NGKIHIGHAL NKILKDMIVR SKTLAGFDAP YVPGWDCHGL
110 120 130 140 150
PIEHKVEVTH GKHLSADRTR ELCREYAAEQ IEGQKTEFIR LGVLGDWDNP
160 170 180 190 200
YKTMNFANEA GEIRALAEMV KQGFVFKGLK PVNWCFDCGS ALAEAEVEYA
210 220 230 240 250
DKKSQTIDVA FPVADDAKLA AAFGLASLAK PAAIVIWTTT PWTIPANQAL
260 270 280 290 300
NIHPEFKYAL VDTGERLLVL AEELVESCLK RYSLEGSVIA TAQGSALELI
310 320 330 340 350
NFRHPFYDRL SPIYLADYVE LGAGTGVVHS SPAYGEDDFV TCKRYGMVND
360 370 380 390 400
DILTPVQSNG VYVDSLPFFG GQFIWKANPA IVEKLSEVGA LMHTETISHS
410 420 430 440 450
YMHCWRHKTP LIYRATAQWF VGMDKQPTTG EPLRERALKA IEDTKFVPAW
460 470 480 490 500
GQARLHSMIA NRPDWCISRQ RNWGVPIPFF LHKQTGELHP RTVELMEAVA
510 520 530 540 550
KRVEQEGIEA WFKLDAAELL GAEAEQYDKI TDTLDVWFDS GTTHWHVLRG
560 570 580 590 600
SHDIGHATGP RADLYLEGSD QHRGWFHSSL LTGCAIDGHA PYRELLTHGF
610 620 630 640 650
TVDENGRKMS KSLGNTIEPE KVNNTLGADI LRLWVAATDY SGEMAVSEQI
660 670 680 690 700
LQRSADAYRR IRNTARFLLS NLSGFDPARD LLAPEDMLAL DRWAVDRTLL
710 720 730 740 750
LQRELEEHYS EYRFWNVYSK VHNFCVQELG GFYLDIIKDR QYTTGANSVA
760 770 780 790 800
RRSCQTALYH ISEALVRWIA PILAFTADEI WQYLPGERNE SVMLNGWYEG
810 820 830 840 850
LSELPVGTEL DRAYWDRVMA VKASVNKELE NQRTAKVIGG NLQAEVTLYA
860 870 880 890 900
DEGLSADLGK LGDELRFVLI TSAASVVPFA QAPAEAVATE VEGLKLQVVK
910 920 930 940
SGHTKCGRCW HFRADVGSHP EHPEICGRCV DNLSGSGEVR HYA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP010359 Genomic DNA Translation: AJG15906.1 NTME01000053 Genomic DNA Translation: PBJ92321.1 |
RefSeqi | WP_013970751.1, NZ_NTME01000053.1 |
Genome annotation databases
EnsemblBacteriai | AJG15906; AJG15906; RK21_04398 KGK23591; KGK23591; GT93_01580 |
KEGGi | ppj:RK21_04398 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP010359 Genomic DNA Translation: AJG15906.1 NTME01000053 Genomic DNA Translation: PBJ92321.1 |
RefSeqi | WP_013970751.1, NZ_NTME01000053.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Genome annotation databases
EnsemblBacteriai | AJG15906; AJG15906; RK21_04398 KGK23591; KGK23591; GT93_01580 |
KEGGi | ppj:RK21_04398 |
Family and domain databases
CDDi | cd07960, Anticodon_Ia_Ile_BEm, 1 hit |
Gene3Di | 3.40.50.620, 1 hit 3.90.740.10, 1 hit |
HAMAPi | MF_02002, Ile_tRNA_synth_type1, 1 hit |
InterProi | View protein in InterPro IPR001412, aa-tRNA-synth_I_CS IPR002300, aa-tRNA-synth_Ia IPR033708, Anticodon_Ile_BEm IPR002301, Ile-tRNA-ligase IPR023585, Ile-tRNA-ligase_type1 IPR013155, M/V/L/I-tRNA-synth_anticd-bd IPR014729, Rossmann-like_a/b/a_fold IPR009080, tRNAsynth_Ia_anticodon-bd IPR009008, Val/Leu/Ile-tRNA-synth_edit IPR010663, Znf_FPG/IleRS |
Pfami | View protein in Pfam PF08264, Anticodon_1, 1 hit PF00133, tRNA-synt_1, 1 hit PF06827, zf-FPG_IleRS, 1 hit |
PRINTSi | PR00984, TRNASYNTHILE |
SUPFAMi | SSF47323, SSF47323, 1 hit SSF50677, SSF50677, 1 hit |
TIGRFAMsi | TIGR00392, ileS, 1 hit |
PROSITEi | View protein in PROSITE PS00178, AA_TRNA_LIGASE_I, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | A0A099MV40_9PSED | |
Accessioni | A0A099MV40Primary (citable) accession number: A0A099MV40 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | January 7, 2015 |
Last sequence update: | January 7, 2015 | |
Last modified: | December 2, 2020 | |
This is version 39 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |