UniProtKB - A0A097ZPE8 (ANDI_EMEVA)
Short chain dehydrogenase andI
andI
Functioni
Short chain dehydrogenase; part of the gene cluster that mediates the biosynthesis of anditomin, a fungal meroterpenoid (PubMed:25216349).
The first step of the pathway is the synthesis of 3,5-dimethylorsellinic acid (DMOA) by the polyketide synthase andM (PubMed:25216349).
DMOA is then converted to the phthalide compound 5,7-dihydroxy-4,6-dimethylphthalide (DHDMP) by the cytochrome P450 monooxygenase andK, which is further prenylated by the prenyltransferase andD to yield farnesyl-DHDMP (PubMed:25216349).
Further epoxidation by the FAD-dependent monooxygenase andE leads to epoxyfarnesyl-DHDMP (PubMed:25216349).
The next step involves the terpene cyclase andB that converts epoxyfarnesyl-DHDMP into preandiloid A through opening of the epoxide ring followed by the cyclization of the farnesyl moiety (PubMed:25216349).
Preandiloid A is in turn oxidized at the C-3 hydroxyl group to yield preandiloid B by the dehydrogenase andC (PubMed:25216349).
The dioxygenase andA is solely responsible for the dehydrogenation of preandiloid B leading to the enone preandiloid C, as well as for the intriguing structural rearrangement to generate the bicyclo[2.2.2]octane core, transforming preandiloid C into andiconin (PubMed:25216349).
FAD-binding monooxygenase andJ then produces andilesin D which is reduced by dehydrogenase andI to yield andilesin A (PubMed:25216349).
Action of acetyltransferase andG followed by a spontaneous acetate elimination leads then to andilesin B, which is in turn substrate of the short chain dehydrogenase andH to yield andilesin C (PubMed:25216349).
Finally, the dioxygenase andF catalyzes the transformation of andilesin C to anditomin (PubMed:25216349).
1 Publication: terpenoid biosynthesis Pathwayi
This protein is involved in the pathway terpenoid biosynthesis, which is part of Secondary metabolite biosynthesis.1 PublicationView all proteins of this organism that are known to be involved in the pathway terpenoid biosynthesis and in Secondary metabolite biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 186 | Proton acceptorPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- oxidoreductase activity Source: UniProtKB-KW
GO - Biological processi
- terpenoid biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Oxidoreductase |
Enzyme and pathway databases
UniPathwayi | UPA00213 |
Names & Taxonomyi
Protein namesi | Recommended name: Short chain dehydrogenase andI1 Publication (EC:1.1.1.-1 Publication)Alternative name(s): Anditomin synthesis protein I1 Publication |
Gene namesi | Name:andI1 Publication |
Organismi | Emericella variicolor (Aspergillus stellatus) |
Taxonomic identifieri | 1549217 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Aspergillaceae › Aspergillus |
Pathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000436585 | 1 – 290 | Short chain dehydrogenase andIAdd BLAST | 290 |
Family & Domainsi
Sequence similaritiesi
Family and domain databases
InterProi | View protein in InterPro IPR036291, NAD(P)-bd_dom_sf IPR002347, SDR_fam |
Pfami | View protein in Pfam PF00106, adh_short, 1 hit |
PRINTSi | PR00081, GDHRDH PR00080, SDRFAMILY |
SUPFAMi | SSF51735, SSF51735, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MEKAVSQFKD FVQSQHHDSY AAIQQADHRG HVVAITGASS GIGKAIAVSY
60 70 80 90 100
GRCGVSALVL IARRPLDDVK STVIAASVAA GHSPPEVMTV AANVADETSI
110 120 130 140 150
YLAATKVKET LGHIDILVNN AGRVGTWGNV EAGEAQDWWQ TWEVNIKGVY
160 170 180 190 200
LTTRAFIPML LQGKQKTIVN IGSMGVVSPV ANLSAYLPSK LALIQFTNIV
210 220 230 240 250
ALEYSSQGLV AFTAYPGDVA TELTGSFPEA MSPYFVDTPE LPGDSIAWLT
260 270 280 290
QRRLEWLSGR FVSLRWDLPE LVSRQSEIVE GDKLRVTMRV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB981314 Genomic DNA Translation: BAP81863.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB981314 Genomic DNA Translation: BAP81863.1 |
3D structure databases
AlphaFoldDBi | A0A097ZPE8 |
SMRi | A0A097ZPE8 |
ModBasei | Search... |
Enzyme and pathway databases
UniPathwayi | UPA00213 |
Family and domain databases
InterProi | View protein in InterPro IPR036291, NAD(P)-bd_dom_sf IPR002347, SDR_fam |
Pfami | View protein in Pfam PF00106, adh_short, 1 hit |
PRINTSi | PR00081, GDHRDH PR00080, SDRFAMILY |
SUPFAMi | SSF51735, SSF51735, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ANDI_EMEVA | |
Accessioni | A0A097ZPE8Primary (citable) accession number: A0A097ZPE8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 6, 2016 |
Last sequence update: | January 7, 2015 | |
Last modified: | May 25, 2022 | |
This is version 16 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Documents
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families