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Protein

Lipoyl synthase, mitochondrial

Gene

O988_06941

Organism
Pseudogymnoascus sp. VKM F-3808
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Catalytic activityi

Protein N6-(octanoyl)lysine + an [Fe-S] cluster scaffold protein carrying a [4Fe-4S]2+ cluster + 2 S-adenosyl-L-methionine + 2 oxidized [2Fe-2S] ferredoxin + 6 H+ = protein N6-(dihydrolipoyl)lysine + an [Fe-S] cluster scaffold protein + 2 sulfide + 4 Fe3+ + 2 L-methionine + 2 5'-deoxyadenosine + 2 reduced [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi: protein lipoylation via endogenous pathway

This protein is involved in step 2 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Lipoyl synthase, mitochondrial (O988_06941)
This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi139Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi144Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi150Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi170Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi174Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi177Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferaseUniRule annotation
Ligand4Fe-4SUniRule annotation, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionineUniRule annotation

Enzyme and pathway databases

UniPathwayi
UPA00538;UER00593

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lipoate synthaseUniRule annotation
Short name:
LSUniRule annotation
Short name:
Lip-synUniRule annotation
Lipoic acid synthaseUniRule annotation
Gene namesi
ORF Names:O988_06941Imported
OrganismiPseudogymnoascus sp. VKM F-3808Imported
Taxonomic identifieri1391699 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaLeotiomycetesLeotiomycetes incertae sedisPseudeurotiaceaePseudogymnoascus
Proteomesi
  • UP000029329 Componenti: Unassembled WGS sequence

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

MitochondrionUniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini160 – 380Elp3InterPro annotationAdd BLAST221

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00206 Lipoyl_synth, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006638 Elp3/MiaB/NifB
IPR031691 LIAS_N
IPR003698 Lipoyl_synth
IPR007197 rSAM
PfamiView protein in Pfam
PF16881 LIAS_N, 1 hit
PF04055 Radical_SAM, 1 hit
SFLDiSFLDF00271 lipoyl_synthase, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00510 lipA, 1 hit

Sequencei

Sequence statusi: Complete.

A0A093XKK1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MYAMASSSSR FRCLNAPPLR NAAQAIYSKS TTTRGLATPA GEPPAADNTT
60 70 80 90 100
TPSTAAPKKG RPSYFKDSPV PTLHEYLDID QPLTPAEMLA LRPRTAKVGK
110 120 130 140 150
KEITRLPSWL KTSIPSPNTS AGFKAIKADL RGLGLHTVCE EARCPNIGEC
160 170 180 190 200
WGGSDKASAT ATIMLMGDTC TRGCRFCSVK TARKAKGAWD MAPLDPHEPE
210 220 230 240 250
NTAEALRRWG LGYVVLTSVD RDDLPDGGAA HFAETIAKIK SKSRENGGRE
260 270 280 290 300
ILVEALTGDF GGQLDMVDKV AMSGLDVYAH NVETVDELTP QVRDRRATFA
310 320 330 340 350
QSLKVLGRAK ETVPGLITKT SIMLGLGETE PQLMDALRRL REVNVDVVTF
360 370 380 390 400
GQYMRPTKRH MQVEEYITPQ VFEAWRKRAL EMGFLYCAAG PLVRSSYKAG
410 420
EAFIENVLKQ RKGKVEGETK VEEKVVEV
Length:428
Mass (Da):46,819
Last modified:November 26, 2014 - v1
Checksum:i899F485E16C23032
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
JPJR01001549 Genomic DNA Translation: KFX93193.1

Genome annotation databases

EnsemblFungiiKFX93193; KFX93193; O988_06941

Similar proteinsi

Entry informationi

Entry nameiA0A093XKK1_9PEZI
AccessioniPrimary (citable) accession number: A0A093XKK1
Entry historyiIntegrated into UniProtKB/TrEMBL: November 26, 2014
Last sequence update: November 26, 2014
Last modified: July 18, 2018
This is version 19 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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