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Protein

2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Gene

ispD

Organism
Bacillus mycoides
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP).UniRule annotationSAAS annotation

Catalytic activityi

CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol.UniRule annotationSAAS annotation

Pathwayi: isopentenyl diphosphate biosynthesis via DXP pathway

This protein is involved in step 2 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate.UniRule annotationSAAS annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr), 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr), 1-deoxy-D-xylulose-5-phosphate reductoisomerase (BTH41_00969), 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr), 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr), 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr), 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr), 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr), 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr), 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr), 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr), 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr), 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr), 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr), 1-deoxy-D-xylulose-5-phosphate reductoisomerase (COC54_26250), 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr), 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr), 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DJ92_316), 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr), 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr), 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr), 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr), 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr), 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr)
  2. 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD), 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD), 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD), 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD), 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD), 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD), 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD), 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD), 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD), 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD), 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD), 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD), 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD), 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD)
  3. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE), 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE), 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE), 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE), 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ipk), 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ipk), 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE), 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE), 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE), 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ipk), 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE), 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE)
  4. 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF), 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF), 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF), 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF), 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF), 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF), 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF), 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF)
  5. 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG), 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG), 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG), 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG), 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG), 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG), 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG), 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG), 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG), 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG), 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG), 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG), 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG), 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG), 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG)
  6. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH), 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH), 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH), 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH), 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH), 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH), 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH), 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH), 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)
This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via DXP pathway, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei14Transition state stabilizerUniRule annotation1
Sitei21Transition state stabilizerUniRule annotation1
Sitei151Positions MEP for the nucleophilic attackUniRule annotation1
Sitei207Positions MEP for the nucleophilic attackUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferaseUniRule annotationSAAS annotationImported, Transferase
Biological processIsoprene biosynthesisUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayi
UPA00056;UER00093

Names & Taxonomyi

Protein namesi
Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferaseUniRule annotation (EC:2.7.7.60UniRule annotation)
Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthaseUniRule annotation
MEP cytidylyltransferaseUniRule annotation
Short name:
MCTUniRule annotation
Gene namesi
Name:ispDUniRule annotationImported
ORF Names:AWW70_02340Imported, bcere0007_710Imported, BG05_403Imported, BW900_23680Imported, bwei_5589Imported, BWINRA5_00547Imported, COL41_25220Imported, COL71_28030Imported, III_05187Imported, S3E15_01702Imported
OrganismiBacillus mycoidesImported
Taxonomic identifieri1405 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000196040 Componenti: Unassembled WGS sequence
  • UP000194131 Componenti: Unassembled WGS sequence
  • UP000190696 Componenti: Unassembled WGS sequence
  • UP000001909 Componenti: Chromosome
  • UP000065797 Componenti: Unassembled WGS sequence
  • UP000031884 Componenti: Chromosome
  • UP000006976 Componenti: Unassembled WGS sequence
  • UP000237521 Componenti: Unassembled WGS sequence
  • UP000030313 Componenti: Chromosome
  • UP000222321 Componenti: Unassembled WGS sequence

Family & Domainsi

Sequence similaritiesi

Belongs to the IspD/TarI cytidylyltransferase family. IspD subfamily.UniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4105CE5 Bacteria
COG1211 LUCA
KOiK00991
OrthoDBiPOG091H066R

Family and domain databases

CDDicd02516 CDP-ME_synthetase, 1 hit
Gene3Di3.90.550.10, 1 hit
HAMAPiMF_00108 IspD, 1 hit
InterProiView protein in InterPro
IPR001228 IspD
IPR034683 IspD/TarI
IPR018294 ISPD_synthase_CS
IPR029044 Nucleotide-diphossugar_trans
PfamiView protein in Pfam
PF01128 IspD, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit
TIGRFAMsiTIGR00453 ispD, 1 hit
PROSITEiView protein in PROSITE
PS01295 ISPD, 1 hit

Sequencei

Sequence statusi: Complete.

A0A084ITK2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MYTLIIPAAG QGKRMGAGKN KLFLLINGVP IIVHTLRAFE KDKACKRIIM
60 70 80 90 100
AINEEERPYF EELMQKYPVE KQVQFIQGGA ERQDSVYNAL QYVSGVEYVL
110 120 130 140 150
VHDGARPFVT NKMMQDVLTA AEKYGASICA VPVKDTIKKV EQGVVVETVE
160 170 180 190 200
RSQLKAVQTP QGFSVPLLLE AHRSAKQSCF LGTDDASLVE RVGKKVGVVE
210 220
GSYYNIKVTT PEDLLIAESF LHVQKK
Length:226
Mass (Da):25,134
Last modified:October 29, 2014 - v1
Checksum:i508EE0BE601E54BF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP009746 Genomic DNA Translation: AIW88100.1
CP009692 Genomic DNA Translation: AJH17290.1
ACLX01000004 Genomic DNA Translation: EEK75367.1
AHEV01000039 Genomic DNA Translation: EJR32468.1
LRPH01000066 Genomic DNA Translation: KWU59892.1
MUAI01000028 Genomic DNA Translation: OOR04165.1
MRWU01000017 Genomic DNA Translation: OSX90457.1
NVFI01000029 Genomic DNA Translation: PFX91839.1
NVGU01000034 Genomic DNA Translation: PGA04246.1
FLZU01000005 Genomic DNA Translation: SCA97214.1
RefSeqiWP_002009811.1, NZ_NVGU01000034.1

Genome annotation databases

EnsemblBacteriaiAIW88100; AIW88100; bwei_5589
EEK75367; EEK75367; bcere0007_710
EJR32468; EJR32468; III_05187
KEZ80036; KEZ80036; ER45_26310
GeneIDi29400800
KEGGibmyo:BG05_403
bww:bwei_5589
PATRICifig|1053227.3.peg.5315
fig|1405.14.peg.3419
fig|86662.17.peg.5557

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP009746 Genomic DNA Translation: AIW88100.1
CP009692 Genomic DNA Translation: AJH17290.1
ACLX01000004 Genomic DNA Translation: EEK75367.1
AHEV01000039 Genomic DNA Translation: EJR32468.1
LRPH01000066 Genomic DNA Translation: KWU59892.1
MUAI01000028 Genomic DNA Translation: OOR04165.1
MRWU01000017 Genomic DNA Translation: OSX90457.1
NVFI01000029 Genomic DNA Translation: PFX91839.1
NVGU01000034 Genomic DNA Translation: PGA04246.1
FLZU01000005 Genomic DNA Translation: SCA97214.1
RefSeqiWP_002009811.1, NZ_NVGU01000034.1

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAIW88100; AIW88100; bwei_5589
EEK75367; EEK75367; bcere0007_710
EJR32468; EJR32468; III_05187
KEZ80036; KEZ80036; ER45_26310
GeneIDi29400800
KEGGibmyo:BG05_403
bww:bwei_5589
PATRICifig|1053227.3.peg.5315
fig|1405.14.peg.3419
fig|86662.17.peg.5557

Phylogenomic databases

eggNOGiENOG4105CE5 Bacteria
COG1211 LUCA
KOiK00991
OrthoDBiPOG091H066R

Enzyme and pathway databases

UniPathwayi
UPA00056;UER00093

Family and domain databases

CDDicd02516 CDP-ME_synthetase, 1 hit
Gene3Di3.90.550.10, 1 hit
HAMAPiMF_00108 IspD, 1 hit
InterProiView protein in InterPro
IPR001228 IspD
IPR034683 IspD/TarI
IPR018294 ISPD_synthase_CS
IPR029044 Nucleotide-diphossugar_trans
PfamiView protein in Pfam
PF01128 IspD, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit
TIGRFAMsiTIGR00453 ispD, 1 hit
PROSITEiView protein in PROSITE
PS01295 ISPD, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiA0A084ITK2_BACMY
AccessioniPrimary (citable) accession number: A0A084ITK2
Secondary accession number(s): A0A0B5RR85, C2PPV6, J8HXZ8
Entry historyiIntegrated into UniProtKB/TrEMBL: October 29, 2014
Last sequence update: October 29, 2014
Last modified: October 10, 2018
This is version 37 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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