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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Pseudomonas aeruginosa
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen metabolism.UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotationSAAS annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotationSAAS annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolaseUniRule annotationSAAS annotation, Multifunctional enzymeUniRule annotationSAAS annotation, NucleotidyltransferaseUniRule annotationSAAS annotationImported, Transferase
LigandMagnesiumUniRule annotationSAAS annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
Gene namesi
Name:glnDUniRule annotationImported
ORF Names:AO964_02075Imported, PAMH19_1429Imported
OrganismiPseudomonas aeruginosaImported
Taxonomic identifieri287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000043988 Componenti: Unassembled WGS sequence
  • UP000054840 Componenti: Unassembled WGS sequence

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini461 – 583HDInterPro annotationAdd BLAST123
Domaini706 – 789ACTInterPro annotationAdd BLAST84
Domaini816 – 891ACTInterPro annotationAdd BLAST76

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 342UridylyltransferaseUniRule annotationAdd BLAST342

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotationSAAS annotation

Keywords - Domaini

RepeatSAAS annotation

Phylogenomic databases

eggNOGiENOG4105E1P Bacteria
COG2844 LUCA

Family and domain databases

CDDicd00077 HDc, 1 hit
HAMAPiMF_00277 PII_uridylyl_transf, 1 hit
InterProiView protein in InterPro
IPR002912 ACT_dom
IPR003607 HD/PDEase_dom
IPR006674 HD_domain
IPR013546 PII_UdlTrfase/GS_AdlTrfase
IPR002934 Polymerase_NTP_transf_dom
IPR010043 UTase/UR
PANTHERiPTHR13734:SF45 PTHR13734:SF45, 1 hit
PfamiView protein in Pfam
PF01842 ACT, 1 hit
PF08335 GlnD_UR_UTase, 1 hit
PF01966 HD, 1 hit
PF01909 NTP_transf_2, 1 hit
PIRSFiPIRSF006288 PII_uridyltransf, 1 hit
SMARTiView protein in SMART
SM00471 HDc, 1 hit
TIGRFAMsiTIGR01693 UTase_glnD, 1 hit
PROSITEiView protein in PROSITE
PS51671 ACT, 2 hits
PS51831 HD, 1 hit

Sequencei

Sequence statusi: Complete.

A0A081HN09-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPQVDPELFD RGQFQAELAL KSSPIAAFKK AIRQFREVLD NRFNSGRDIR
60 70 80 90 100
RLIEDRAWCV DQILQQAWQR FDWGDDADIA LVAVGGYGRG ELHPYSDVDL
110 120 130 140 150
LILLDSEDQE SFREPIEGFL TLLWDIGLEV GQSVRSVQQC AEEARADLTV
160 170 180 190 200
ITTLMECRTI CGPDSLRQRM LRVTGSAHMW PSKEFFLAKR HEQQRRHAKY
210 220 230 240 250
NDTEYNLEPN VKGSPGGLRD IQTILWMARR QFGSLNLHAL VREGFLVESE
260 270 280 290 300
CSMLASSQEF LWRVRYALHM LAGRAEDRLL FDHQRSIARL FGYEDNDVKL
310 320 330 340 350
AVERFMQKYY RVVMAISELN DLIIQHFEEV ILPCEQPVQI QPLNSRFQLR
360 370 380 390 400
DGYIEVTHPN VFKRTPFALL EIFVLMAQHP EIKGVRADTI RLLRDSRHLI
410 420 430 440 450
DDEFRHDIRN TSLFIELFKS SQGIHRNLRR MNRYGILGRY LPEFGHIIGQ
460 470 480 490 500
MQHDLFHIYT VDAHTLNLIK HLRKLNRPEM AEKYPLASKI IDRLPKPELI
510 520 530 540 550
YIAGLYHDIA KGRGGDHSEL GAVDAEAFCQ SHQLPLWDTQ LVSWLVQNHL
560 570 580 590 600
VMSTTAQRKD LSDPQVIFDF AQLVGDQTHL DYLYVLTVAD INATNPTLWN
610 620 630 640 650
SWRASLLRQL YTETKRALRR GLENPVDREE QIRQTQTAAL DQLVRNGIDQ
660 670 680 690 700
DDAEQLWSQL GDDYFLRHTA GDVAWHTEAI LQHPDDGTPL VLIKETTQRE
710 720 730 740 750
FESGSQIFIY AADQHDFFAV TVAAMDQLNL SIQDARIITS TSQFTLDTYI
760 770 780 790 800
VLDADGDSIG NNPERIAEIR EGLIDALKNP DDYPTIIQRR VPRQLKHFAF
810 820 830 840 850
APQVTISTDA LRQVSVLEVI APDRPGLLAR IGGIFLDFDL SVQNAKIATL
860 870 880 890 900
GERVEDVFYI TDARNQPLAD PDLCKRLQAA LVEQLSQDNG RDTLPTRINF
Length:900
Mass (Da):103,432
Last modified:October 29, 2014 - v1
Checksum:i3D05096DA92480CF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CDAZ01000004 Genomic DNA Translation: CEI05277.1
LLOD01000001 Genomic DNA Translation: KSH18461.1
RefSeqiWP_003098610.1, NZ_QBES01000003.1

Genome annotation databases

EnsemblBacteriaiCEI05277; CEI05277; PAMH19_1429
KSH18461; KSH18461; AO964_02075
PATRICifig|287.1481.peg.1816

Similar proteinsi

Entry informationi

Entry nameiA0A081HN09_PSEAI
AccessioniPrimary (citable) accession number: A0A081HN09
Entry historyiIntegrated into UniProtKB/TrEMBL: October 29, 2014
Last sequence update: October 29, 2014
Last modified: July 18, 2018
This is version 35 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

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