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Protein

Inosine-5'-monophosphate dehydrogenase

Gene

guaB

Organism
Bacillus mycoides
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Catalytic activityi

Inosine 5'-phosphate + NAD+ + H2O = xanthosine 5'-phosphate + NADH.UniRule annotation

Cofactori

K+UniRule annotation

Enzyme regulationi

Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.UniRule annotation

Pathwayi: XMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes XMP from IMP.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Inosine-5'-monophosphate dehydrogenase (guaB), Inosine-5'-monophosphate dehydrogenase (guaB), Inosine-5'-monophosphate dehydrogenase (guaB), Inosine-5'-monophosphate dehydrogenase (guaB2), Inosine-5'-monophosphate dehydrogenase (guaB), Inosine-5'-monophosphate dehydrogenase (guaB), Inosine-5'-monophosphate dehydrogenase (guaB), Inosine-5'-monophosphate dehydrogenase (guaB), Inosine-5'-monophosphate dehydrogenase (guaB), Inosine-5'-monophosphate dehydrogenase (guaB), Inosine-5'-monophosphate dehydrogenase (guaB), Inosine-5'-monophosphate dehydrogenase (guaB)
This subpathway is part of the pathway XMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes XMP from IMP, the pathway XMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei251NADUniRule annotation1
Metal bindingi303Potassium; via carbonyl oxygenUniRule annotation1
Metal bindingi305Potassium; via carbonyl oxygenUniRule annotation1
Binding sitei306IMPUniRule annotation1
Active sitei308Thioimidate intermediateUniRule annotation1
Metal bindingi308Potassium; via carbonyl oxygenUniRule annotation1
Active sitei404Proton acceptorUniRule annotation1
Binding sitei416IMPUniRule annotation1
Metal bindingi470Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation1
Metal bindingi471Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation1
Metal bindingi472Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi251 – 253NADUniRule annotation3
Nucleotide bindingi301 – 303NADUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductaseUniRule annotationImported
Biological processGMP biosynthesisUniRule annotation, Purine biosynthesis
LigandMetal-bindingUniRule annotation, NADUniRule annotation, PotassiumUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00601; UER00295

Names & Taxonomyi

Protein namesi
Recommended name:
Inosine-5'-monophosphate dehydrogenaseUniRule annotation (EC:1.1.1.205UniRule annotation)
Short name:
IMP dehydrogenaseUniRule annotation
Short name:
IMPDUniRule annotation
Short name:
IMPDHUniRule annotation
Gene namesi
Name:guaBUniRule annotationImported
ORF Names:DJ92_2597Imported
OrganismiBacillus mycoidesImported
Taxonomic identifieri1405 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000028949 Componenti: Chromosome

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini95 – 153CBSInterPro annotationAdd BLAST59
Domaini157 – 215CBSInterPro annotationAdd BLAST59

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni341 – 343IMP bindingUniRule annotation3
Regioni364 – 365IMP bindingUniRule annotation2
Regioni388 – 392IMP bindingUniRule annotation5

Sequence similaritiesi

Belongs to the IMPDH/GMPR family.UniRule annotation

Keywords - Domaini

CBS domainPROSITE-ProRule annotation

Phylogenomic databases

KOiK00088

Family and domain databases

CDDicd00381 IMPDH, 1 hit
Gene3Di3.20.20.70, 1 hit
HAMAPiMF_01964 IMPDH, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR000644 CBS_dom
IPR005990 IMP_DH
IPR015875 IMP_DH/GMP_Rdtase_CS
IPR001093 IMP_DH_GMPRt
PfamiView protein in Pfam
PF00571 CBS, 2 hits
PF00478 IMPDH, 1 hit
PIRSFiPIRSF000130 IMPDH, 1 hit
SMARTiView protein in SMART
SM00116 CBS, 2 hits
TIGRFAMsiTIGR01302 IMP_dehydrog, 1 hit
PROSITEiView protein in PROSITE
PS51371 CBS, 2 hits
PS00487 IMP_DH_GMP_RED, 1 hit

Sequencei

Sequence statusi: Complete.

A0A076W2H6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWESKFVKEG LTFDDVLLVP AKSDVLPREV SVKTVLSESL QLNIPLISAG
60 70 80 90 100
MDTVTEADMA IAMARQGGLG VIHKNMSIEQ QAEQVDKVKR SESGVISDPF
110 120 130 140 150
FLTPDHQVYD AEHLMGKYRI SGVPIVNNLE EQKLVGIITN RDMRFIQDYS
160 170 180 190 200
IKISDVMTKE KLITAPVGTT LEEAEKILQK YKIEKLPLVD NNGVLQGLIT
210 220 230 240 250
IKDIEKVIEF PNSAKDKQGR LLVGAAVGVT ADAILRIDAL VKANVDVIVL
260 270 280 290 300
DTAHGHSQGV IEKVKEVRAK YPTLNIIAGN VATAEATRAL IEAGANVIKV
310 320 330 340 350
GIGPGSICTT RVVAGVGVPQ LTAVYDCATE ARKHGIPVIA DGGIKYSGDM
360 370 380 390 400
VKALAAGAHV VMLGSMFAGV AESPGETEIY QGRQFKVYRG MGSVGAMEKG
410 420 430 440 450
SKDRYFQEGN KKLVPEGIEG RVPYKGPLAD TVHQLVGGLR AGMGYCGAND
460 470 480
LEFLRENAQF IRMSGAGLRE SHPHHVQITK EAPNYSL
Length:487
Mass (Da):52,537
Last modified:October 29, 2014 - v1
Checksum:i1FE443978556D55B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP007626 Genomic DNA Translation: AIK36906.1
RefSeqiWP_018783552.1, NZ_JMQD01000083.1

Genome annotation databases

EnsemblBacteriaiAIK36906; AIK36906; DJ92_2597
GeneIDi34215902
KEGGibmyc:DJ92_2597
PATRICifig|1405.13.peg.2686

Similar proteinsi

Entry informationi

Entry nameiA0A076W2H6_BACMY
AccessioniPrimary (citable) accession number: A0A076W2H6
Entry historyiIntegrated into UniProtKB/TrEMBL: October 29, 2014
Last sequence update: October 29, 2014
Last modified: March 28, 2018
This is version 24 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

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