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Protein

Thymidylate synthase

Gene

thyA

Organism
Enterococcus faecalis 918
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotationSAAS annotation

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei22dUMPUniRule annotation1
Active sitei197PROSITE-ProRule annotation1
Active sitei197NucleophileUniRule annotation1
Binding sitei2205,10-methylenetetrahydrofolateUniRule annotation1
Binding sitei228dUMPUniRule annotation1
Binding sitei3145,10-methylenetetrahydrofolate; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi177 – 178dUMP; shared with dimeric partnerUniRule annotation2
Nucleotide bindingi217 – 220dUMPUniRule annotation4
Nucleotide bindingi258 – 260dUMPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferaseUniRule annotationSAAS annotation, Transferase
Biological processNucleotide biosynthesisUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayi
UPA00575

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotationSAAS annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotation
ORF Names:HMPREF2097_00128Imported
OrganismiEnterococcus faecalis 918Imported
Taxonomic identifieri1338519 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus
Proteomesi
  • UP000026654 Componenti: Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Structurei

3D structure databases

ProteinModelPortaliA0A059N5Z7
SMRiA0A059N5Z7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 315Thymidylat_syntInterPro annotationAdd BLAST312

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Family and domain databases

CDDicd00351 TS_Pyrimidine_HMase, 1 hit
Gene3Di3.30.572.10, 1 hit
HAMAPiMF_00008 Thymidy_synth_bact, 1 hit
InterProiView protein in InterPro
IPR023451 Thymidate_synth/dCMP_Mease
IPR036926 Thymidate_synth/dCMP_Mease_sf
IPR000398 Thymidylate_synthase
IPR020940 Thymidylate_synthase_AS
PfamiView protein in Pfam
PF00303 Thymidylat_synt, 1 hit
PRINTSiPR00108 THYMDSNTHASE
SUPFAMiSSF55831 SSF55831, 1 hit
TIGRFAMsiTIGR03284 thym_sym, 1 hit
PROSITEiView protein in PROSITE
PS00091 THYMIDYLATE_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

A0A059N5Z7-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEEAYLALGK KILEEGHFKE DRTGTGTYSL FGYQMRFDLA KGFPLLTTKR
60 70 80 90 100
VPFGLIKSEL LWFLKGDTNI RYLLERNNHI WDEWAFERYV KSADYQGPDM
110 120 130 140 150
TDFGHRVLQD PAFAEQYKEE HQKFCDAILN DAEFAEKYGE LGNIYGAQWR
160 170 180 190 200
HWETKDGSFI DQLANVIEMI KTNPDSRRLI VSAWNPEDVP SMALPPCHTM
210 220 230 240 250
FQFYVNEGKL SCQLYQRSAD VFLGVPFNIA SYALLTHLIA HETGLEVGEF
260 270 280 290 300
VHTLGDAHLY QNHVEQMQEQ LSREVRSFPT LVLNPDKASV FDFDMEDIKV
310
EGYDPHPTIK APIAV
Length:315
Mass (Da):36,344
Last modified:July 9, 2014 - v1
Checksum:i0593A11D77D2D0A2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AVNY01000006 Genomic DNA Translation: KDE18770.1
RefSeqiWP_002357569.1, NZ_KK640403.1

Genome annotation databases

EnsemblBacteriaiKDE18770; KDE18770; HMPREF2097_00128
PATRICifig|1338519.3.peg.124

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AVNY01000006 Genomic DNA Translation: KDE18770.1
RefSeqiWP_002357569.1, NZ_KK640403.1

3D structure databases

ProteinModelPortaliA0A059N5Z7
SMRiA0A059N5Z7
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiKDE18770; KDE18770; HMPREF2097_00128
PATRICifig|1338519.3.peg.124

Enzyme and pathway databases

UniPathwayi
UPA00575

Family and domain databases

CDDicd00351 TS_Pyrimidine_HMase, 1 hit
Gene3Di3.30.572.10, 1 hit
HAMAPiMF_00008 Thymidy_synth_bact, 1 hit
InterProiView protein in InterPro
IPR023451 Thymidate_synth/dCMP_Mease
IPR036926 Thymidate_synth/dCMP_Mease_sf
IPR000398 Thymidylate_synthase
IPR020940 Thymidylate_synthase_AS
PfamiView protein in Pfam
PF00303 Thymidylat_synt, 1 hit
PRINTSiPR00108 THYMDSNTHASE
SUPFAMiSSF55831 SSF55831, 1 hit
TIGRFAMsiTIGR03284 thym_sym, 1 hit
PROSITEiView protein in PROSITE
PS00091 THYMIDYLATE_SYNTHASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiA0A059N5Z7_ENTFL
AccessioniPrimary (citable) accession number: A0A059N5Z7
Entry historyiIntegrated into UniProtKB/TrEMBL: July 9, 2014
Last sequence update: July 9, 2014
Last modified: November 7, 2018
This is version 27 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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