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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Mycobacterium tuberculosis
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei81SubstrateUniRule annotation1
Metal bindingi229ManganeseUniRule annotation1
Metal bindingi249Manganese; via tele nitrogenUniRule annotation1
Binding sitei271SubstrateUniRule annotation1
Active sitei273UniRule annotation1
Metal bindingi296ManganeseUniRule annotation1
Binding sitei389GTPUniRule annotation1
Binding sitei420GTPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi272 – 277GTPUniRule annotation6
Nucleotide bindingi515 – 518GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDecarboxylaseUniRule annotationSAAS annotation, Lyase
Biological processGluconeogenesisUniRule annotation
LigandGTP-bindingUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayi
UPA00138

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Alternative name(s):
GTP-dependent phosphoenolpyruvate carboxykinaseUniRule annotation
Short name:
GTP-PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotationImported
ORF Names:ERS007663_00470Imported, ERS007665_00107Imported, ERS007670_02650Imported, ERS007679_00458Imported, ERS007681_00383Imported, ERS007703_00825Imported, ERS007722_01757Imported, ERS007741_00434Imported, ERS023446_01746Imported, ERS027651_01148Imported, ERS027653_00735Imported, ERS027654_01518Imported, ERS027661_02744Imported, ERS124361_01655Imported, SAMEA2682864_00024Imported, SAMEA2683035_00367Imported
OrganismiMycobacterium tuberculosisImported
Taxonomic identifieri1773 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000047075 Componenti: Unassembled WGS sequence
  • UP000045842 Componenti: Unassembled WGS sequence
  • UP000041288 Componenti: Unassembled WGS sequence
  • UP000043293 Componenti: Unassembled WGS sequence
  • UP000049023 Componenti: Unassembled WGS sequence
  • UP000048600 Componenti: Unassembled WGS sequence
  • UP000183655 Componenti: Unassembled WGS sequence
  • UP000039953 Componenti: Unassembled WGS sequence
  • UP000182946 Componenti: Unassembled WGS sequence
  • UP000048289 Componenti: Unassembled WGS sequence
  • UP000049258 Componenti: Unassembled WGS sequence
  • UP000038802 Componenti: Unassembled WGS sequence
  • UP000049114 Componenti: Unassembled WGS sequence
  • UP000046213 Componenti: Unassembled WGS sequence
  • UP000045274 Componenti: Unassembled WGS sequence
  • UP000049058 Componenti: Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 240PEPCK_NInterPro annotationAdd BLAST219
Domaini245 – 605PEPCK_CInterPro annotationAdd BLAST361

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni220 – 222Substrate bindingUniRule annotation3
Regioni387 – 389Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Family and domain databases

CDDicd00819 PEPCK_GTP, 1 hit
Gene3Di3.40.449.10, 2 hits
3.90.228.20, 2 hits
HAMAPiMF_00452 PEPCK_GTP, 1 hit
InterProiView protein in InterPro
IPR018091 PEP_carboxykin_GTP_CS
IPR013035 PEP_carboxykinase_C
IPR008209 PEP_carboxykinase_GTP
IPR035077 PEP_carboxykinase_GTP_C
IPR035078 PEP_carboxykinase_GTP_N
IPR008210 PEP_carboxykinase_N
PANTHERiPTHR11561 PTHR11561, 1 hit
PfamiView protein in Pfam
PF00821 PEPCK_C, 1 hit
PF17297 PEPCK_N, 1 hit
PIRSFiPIRSF001348 PEP_carboxykinase_GTP, 1 hit
SUPFAMiSSF68923 SSF68923, 1 hit
PROSITEiView protein in PROSITE
PS00505 PEPCK_GTP, 1 hit

Sequencei

Sequence statusi: Complete.

A0A045I1R8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTSATIPGLD TAPTNHQGLL SWVEEVAELT QPDRVVFTDG SEEEFQRLCD
60 70 80 90 100
QLVEAGTFIR LNPEKHKNSY LALSDPSDVA RVESRTYICS AKEIDAGPTN
110 120 130 140 150
NWMDPGEMRS IMKDLYRGCM RGRTMYVVPF CMGPLGAEDP KLGVEITDSE
160 170 180 190 200
YVVVSMRTMT RMGKAALEKM GDDGFFVKAL HSVGAPLEPG QKDVAWPCSE
210 220 230 240 250
TKYITHFPET REIWSYGSGY GGNALLGKKC YSLRIASAMA HDEGWLAEHM
260 270 280 290 300
LILKLISPEN KAYYFAAAFP SACGKTNLAM LQPTIPGWRA ETLGDDIAWM
310 320 330 340 350
RFGKDGRLYA VNPEFGFFGV APGTNWKSNP NAMRTIAAGN TVFTNVALTD
360 370 380 390 400
DGDVWWEGLE GDPQHLIDWK GNDWYFRETE TNAAHPNSRY CTPMSQCPIL
410 420 430 440 450
APEWDDPQGV PISGILFGGR RKTTVPLVTE ARDWQHGVFI GATLGSEQTA
460 470 480 490 500
AAEGKVGNVR RDPMAMLPFL GYNVGDYFQH WINLGKHADE SKLPKVFFVN
510 520 530 540 550
WFRRGDDGRF LWPGFGENSR VLKWIVDRIE HKAGGATTPI GTVPAVEDLD
560 570 580 590 600
LDGLDVDAAD VAAALAVDAD EWRQELPLIE EWLQFVGEKL PTGVKDEFDA

LKERLG
Length:606
Mass (Da):67,253
Last modified:July 9, 2014 - v1
Checksum:iAEE29412E6BCCAE3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CFOE01000025 Genomic DNA Translation: CFE35811.1
CNFS01000114 Genomic DNA Translation: CKR14428.1
CNFR01000253 Genomic DNA Translation: CKR49597.1
CIEA01000008 Genomic DNA Translation: CKR68798.1
CNFU01000619 Genomic DNA Translation: CKS20673.1
CHDS01000024 Genomic DNA Translation: CNG71366.1
CQGE01000031 Genomic DNA Translation: CNM97110.1
CQPW01000009 Genomic DNA Translation: CNT95664.1
CQPV01000043 Genomic DNA Translation: CNU08849.1
CQPL01000597 Genomic DNA Translation: CNV18033.1
CSAD01000034 Genomic DNA Translation: COU83118.1
CSAE01000057 Genomic DNA Translation: COV21073.1
CHKL01000025 Genomic DNA Translation: COV68396.1
CSAM01000108 Genomic DNA Translation: COW12585.1
FPTZ01000001 Genomic DNA Translation: SGH09838.1
FPXX01000001 Genomic DNA Translation: SGL33784.1
RefSeqiWP_003401212.1, NZ_PYQD01000004.1

Genome annotation databases

EnsemblBacteriaiAMC44504; AMC44504; RN05_0228
CFE35811; CFE35811; ERS007681_00383
CKR14428; CKR14428; ERS027653_00735
CKR49597; CKR49597; ERS027654_01518
CKS20673; CKS20673; ERS027661_02744
CNG71366; CNG71366; ERS124361_01655
CNM97110; CNM97110; ERS023446_01746
CNT95664; CNT95664; ERS007665_00107
CNU08849; CNU08849; ERS007663_00470
CNV18033; CNV18033; ERS007670_02650
CNY35216; CNY35216; ERS013471_01313
COU83118; COU83118; ERS007679_00458
COV21073; COV21073; ERS007703_00825
COV68396; COV68396; ERS007741_00434
COW12585; COW12585; ERS007722_01757
PATRICifig|1773.206.peg.3358

Similar proteinsi

Entry informationi

Entry nameiA0A045I1R8_MYCTX
AccessioniPrimary (citable) accession number: A0A045I1R8
Entry historyiIntegrated into UniProtKB/TrEMBL: July 9, 2014
Last sequence update: July 9, 2014
Last modified: September 12, 2018
This is version 45 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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