UniProtKB - A0A024R4F1 (A0A024R4F1_HUMAN)
Protein
2-phospho-D-glycerate hydro-lyase
Gene
HEL-S-17
Organism
Homo sapiens (Human)
Status
Functioni
Catalytic activityi
- EC:4.2.1.11ARBA annotation
Cofactori
Mg2+ARBA annotation
: glycolysis Pathwayi
This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.ARBA annotationProteins known to be involved in the 5 steps of the subpathway in this organism are:
- Glyceraldehyde-3-phosphate dehydrogenase (HEL-S-278), Glyceraldehyde-3-phosphate dehydrogenase, Glyceraldehyde-3-phosphate dehydrogenase (HEL-S-162eP), Glyceraldehyde-3-phosphate dehydrogenase, testis-specific (GAPDHS), Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
- Phosphoglycerate kinase, Phosphoglycerate kinase 2 (PGK2), Phosphoglycerate kinase, Phosphoglycerate kinase, Phosphoglycerate kinase (HEL-S-68p), Phosphoglycerate kinase 1 (PGK1), Phosphoglycerate kinase (HEL-S-272), Phosphoglycerate kinase, Phosphoglycerate kinase, Phosphoglycerate kinase
- no protein annotated in this organism
- Alpha-enolase (ENO1), Beta-enolase (ENO3), 2-phospho-D-glycerate hydro-lyase, 2-phospho-D-glycerate hydro-lyase, 2-phospho-D-glycerate hydro-lyase (ENO4), 2-phospho-D-glycerate hydro-lyase (HEL-S-17), 2-phospho-D-glycerate hydro-lyase (ENO4), 2-phospho-D-glycerate hydro-lyase (ENO3), 2-phospho-D-glycerate hydro-lyase (ENO1), 2-phospho-D-glycerate hydro-lyase (ENO2), 2-phospho-D-glycerate hydro-lyase (ENO1), 2-phospho-D-glycerate hydro-lyase (ENO3), 2-phospho-D-glycerate hydro-lyase (ENO2), Enolase 4 (ENO4), 2-phospho-D-glycerate hydro-lyase (ENO1), 2-phospho-D-glycerate hydro-lyase (ENO3), 2-phospho-D-glycerate hydro-lyase (ENO1), 2-phospho-D-glycerate hydro-lyase, 2-phospho-D-glycerate hydro-lyase, 2-phospho-D-glycerate hydro-lyase, 2-phospho-D-glycerate hydro-lyase (EDARADD), 2-phospho-D-glycerate hydro-lyase (ENO2), 2-phospho-D-glycerate hydro-lyase (ENO2), 2-phospho-D-glycerate hydro-lyase (ENO3), Gamma-enolase (ENO2)
- Pyruvate kinase, Pyruvate kinase, Pyruvate kinase PKLR (PKLR), Pyruvate kinase, Pyruvate kinase PKM (PKM), Pyruvate kinase (PKM2), Pyruvate kinase (PKLR), Pyruvate kinase (PKM2), Pyruvate kinase (PKM), Pyruvate kinase (PKM), Pyruvate kinase (PKM), Pyruvate kinase (HEL-S-30), Pyruvate kinase (PKM), Pyruvate kinase (PKM), Pyruvate kinase (PKM), Pyruvate kinase (PKM2), Pyruvate kinase (PKM), Pyruvate kinase (PKLR), Pyruvate kinase (PKM2), Pyruvate kinase (PKLR), Pyruvate kinase, Pyruvate kinase (PKM)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 158 | SubstrateUniRule annotation | 1 | |
Binding sitei | 167 | SubstrateUniRule annotation | 1 | |
Active sitei | 210 | Proton donorUniRule annotation | 1 | |
Binding sitei | 293 | SubstrateUniRule annotation | 1 | |
Binding sitei | 318 | SubstrateUniRule annotation | 1 | |
Active sitei | 343 | Proton acceptorUniRule annotation | 1 | |
Binding sitei | 394 | SubstrateUniRule annotation | 1 |
GO - Molecular functioni
- magnesium ion binding Source: InterPro
- phosphopyruvate hydratase activity Source: UniProtKB-EC
GO - Biological processi
- glycolytic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | LyaseARBA annotation |
Biological process | GlycolysisARBA annotation |
Ligand | MagnesiumARBA annotation |
Enzyme and pathway databases
UniPathwayi | UPA00109;UER00187 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:HEL-S-17Imported Synonyms:ENO1Imported ORF Names:hCG_22399Imported |
Organismi | Homo sapiens (Human)Imported |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Organism-specific databases
VEuPathDBi | HostDB:ENSG00000074800.13 |
Subcellular locationi
Cytosol
- cytosol Source: HPA
- phosphopyruvate hydratase complex Source: InterPro
Plasma Membrane
- plasma membrane Source: HPA
Expressioni
Gene expression databases
Bgeei | ENSG00000074800, Expressed in cortex of kidney and 243 other tissues |
ExpressionAtlasi | A0A024R4F1, baseline and differential |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 3 – 134 | Enolase_NInterPro annotationAdd BLAST | 132 | |
Domaini | 142 – 431 | Enolase_CInterPro annotationAdd BLAST | 290 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 370 – 373 | Substrate bindingUniRule annotation | 4 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 83 – 103 | Sequence analysisAdd BLAST | 21 |
Sequence similaritiesi
Belongs to the enolase family.ARBA annotation
Keywords - Domaini
Coiled coilSequence analysisPhylogenomic databases
HOGENOMi | CLU_031223_0_0_1 |
OMAi | GPELVGY |
OrthoDBi | 773373at2759 |
Family and domain databases
CDDi | cd03313, enolase, 1 hit |
Gene3Di | 3.20.20.120, 1 hit 3.30.390.10, 1 hit |
HAMAPi | MF_00318, Enolase, 1 hit |
InterProi | View protein in InterPro IPR000941, Enolase IPR036849, Enolase-like_C_sf IPR029017, Enolase-like_N IPR020810, Enolase_C IPR020809, Enolase_CS IPR020811, Enolase_N |
PANTHERi | PTHR11902, PTHR11902, 1 hit |
Pfami | View protein in Pfam PF00113, Enolase_C, 1 hit PF03952, Enolase_N, 1 hit |
PIRSFi | PIRSF001400, Enolase, 1 hit |
PRINTSi | PR00148, ENOLASE |
SFLDi | SFLDF00002, enolase, 1 hit |
SMARTi | View protein in SMART SM01192, Enolase_C, 1 hit SM01193, Enolase_N, 1 hit |
SUPFAMi | SSF51604, SSF51604, 1 hit SSF54826, SSF54826, 1 hit |
TIGRFAMsi | TIGR01060, eno, 1 hit |
PROSITEi | View protein in PROSITE PS00164, ENOLASE, 1 hit |
i Sequence
Sequence statusi: Complete.
A0A024R4F1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSILKIHARE IFDSRGNPTV EVDLFTSKGL FRAAVPSGAS TGIYEALELR
60 70 80 90 100
DNDKTRYMGK GVSKAVEHIN KTIAPALVSK KLNVTEQEKI DKLMIEMDGT
110 120 130 140 150
ENKSKFGANA ILGVSLAVCK AGAVEKGVPL YRHIADLAGN SEVILPVPAF
160 170 180 190 200
NVINGGSHAG NKLAMQEFMI LPVGAANFRE AMRIGAEVYH NLKNVIKEKY
210 220 230 240 250
GKDATNVGDE GGFAPNILEN KEGLELLKTA IGKAGYTDKV VIGMDVAASE
260 270 280 290 300
FFRSGKYDLD FKSPDDPSRY ISPDQLADLY KSFIKDYPVV SIEDPFDQDD
310 320 330 340 350
WGAWQKFTAS AGIQVVGDDL TVTNPKRIAK AVNEKSCNCL LLKVNQIGSV
360 370 380 390 400
TESLQACKLA QANGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR
410 420 430
SERLAKYNQL LRIEEELGSK AKFAGRNFRN PLAK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | GQ472226 mRNA Translation: ACV87201.1 CH471130 Genomic DNA Translation: EAW71605.1 |
RefSeqi | NP_001419.1, NM_001428.3 |
Genome annotation databases
GeneIDi | 2023 |
KEGGi | hsa:2023 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | GQ472226 mRNA Translation: ACV87201.1 CH471130 Genomic DNA Translation: EAW71605.1 |
RefSeqi | NP_001419.1, NM_001428.3 |
3D structure databases
SMRi | A0A024R4F1 |
ModBasei | Search... |
Chemistry databases
BindingDBi | A0A024R4F1 |
Protocols and materials databases
Antibodypediai | 4351, 834 antibodies |
DNASUi | 2023 |
Genome annotation databases
GeneIDi | 2023 |
KEGGi | hsa:2023 |
Organism-specific databases
CTDi | 2023 |
VEuPathDBi | HostDB:ENSG00000074800.13 |
Phylogenomic databases
HOGENOMi | CLU_031223_0_0_1 |
OMAi | GPELVGY |
OrthoDBi | 773373at2759 |
Enzyme and pathway databases
UniPathwayi | UPA00109;UER00187 |
Miscellaneous databases
BioGRID-ORCSi | 2023, 414 hits in 1012 CRISPR screens |
ChiTaRSi | ENO1, human |
GenomeRNAii | 2023 |
Gene expression databases
Bgeei | ENSG00000074800, Expressed in cortex of kidney and 243 other tissues |
ExpressionAtlasi | A0A024R4F1, baseline and differential |
Family and domain databases
CDDi | cd03313, enolase, 1 hit |
Gene3Di | 3.20.20.120, 1 hit 3.30.390.10, 1 hit |
HAMAPi | MF_00318, Enolase, 1 hit |
InterProi | View protein in InterPro IPR000941, Enolase IPR036849, Enolase-like_C_sf IPR029017, Enolase-like_N IPR020810, Enolase_C IPR020809, Enolase_CS IPR020811, Enolase_N |
PANTHERi | PTHR11902, PTHR11902, 1 hit |
Pfami | View protein in Pfam PF00113, Enolase_C, 1 hit PF03952, Enolase_N, 1 hit |
PIRSFi | PIRSF001400, Enolase, 1 hit |
PRINTSi | PR00148, ENOLASE |
SFLDi | SFLDF00002, enolase, 1 hit |
SMARTi | View protein in SMART SM01192, Enolase_C, 1 hit SM01193, Enolase_N, 1 hit |
SUPFAMi | SSF51604, SSF51604, 1 hit SSF54826, SSF54826, 1 hit |
TIGRFAMsi | TIGR01060, eno, 1 hit |
PROSITEi | View protein in PROSITE PS00164, ENOLASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | A0A024R4F1_HUMAN | |
Accessioni | A0A024R4F1Primary (citable) accession number: A0A024R4F1 Secondary accession number(s): A0A0K0K1K8 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | July 9, 2014 |
Last sequence update: | July 9, 2014 | |
Last modified: | April 7, 2021 | |
This is version 53 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |