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Protein

Fumarate hydratase class II

Gene

fumC

Organism
Escherichia coli
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.UniRule annotation

Miscellaneous

There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.UniRule annotation

Catalytic activityi

(S)-malate = fumarate + H2O.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from fumarate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from fumarate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei187SubstrateUniRule annotation1
Active sitei188Proton donor/acceptorUniRule annotation1
Active sitei318UniRule annotation1
Binding sitei319SubstrateUniRule annotation1
Sitei331Important for catalytic activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseUniRule annotationSAAS annotationImported
Biological processTricarboxylic acid cycleUniRule annotation

Enzyme and pathway databases

UniPathwayi
UPA00223;UER01007

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate hydratase class IIUniRule annotation (EC:4.2.1.2UniRule annotation)
Short name:
Fumarase CUniRule annotation
Alternative name(s):
Aerobic fumaraseUniRule annotation
Iron-independent fumaraseUniRule annotation
Gene namesi
Name:fumCUniRule annotationImported
ORF Names:A8C65_12725Imported, ACU57_22510Imported, AM266_18820Imported, AML35_10540Imported, AUQ13_03900Imported, BHF46_11765Imported, BUE81_02470Imported, BW690_15770Imported, BZL31_05320Imported, C4J69_24605Imported, C5N07_00455Imported, C5P48_07565Imported, CA593_19945Imported, DK267_19110Imported, DK268_19045Imported, DK273_19070Imported, DK278_18760Imported, DK279_18955Imported, DK288_18850Imported, DK289_19045Imported, DL195_07125Imported, EC3234A_33c01110Imported, EL75_2048Imported, EL79_2087Imported, EL80_2114Imported, ERS085386_01435Imported, JD73_22770Imported, PGD_01626Imported, RK56_009470Imported, SK85_01828Imported
OrganismiEscherichia coliImported
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000237650 Componenti: Unassembled WGS sequence
  • UP000245374 Componenti: Unassembled WGS sequence
  • UP000236496 Componenti: Unassembled WGS sequence
  • UP000030018 Componenti: Unassembled WGS sequence
  • UP000194908 Componenti: Unassembled WGS sequence
  • UP000030000 Componenti: Unassembled WGS sequence
  • UP000245481 Componenti: Unassembled WGS sequence
  • UP000246119 Componenti: Unassembled WGS sequence
  • UP000188724 Componenti: Unassembled WGS sequence
  • UP000070045 Componenti: Unassembled WGS sequence
  • UP000245457 Componenti: Unassembled WGS sequence
  • UP000245471 Componenti: Unassembled WGS sequence
  • UP000036012 Componenti: Unassembled WGS sequence
  • UP000076106 Componenti: Unassembled WGS sequence
  • UP000051850 Componenti: Unassembled WGS sequence
  • UP000042887 Componenti: Unassembled WGS sequence
  • UP000028651 Componenti: Unassembled WGS sequence
  • UP000238978 Componenti: Unassembled WGS sequence
  • UP000186204 Componenti: Unassembled WGS sequence
  • UP000246672 Componenti: Unassembled WGS sequence
  • UP000050556 Componenti: Unassembled WGS sequence
  • UP000245666 Componenti: Unassembled WGS sequence
  • UP000196822 Componenti: Unassembled WGS sequence
  • UP000029988 Componenti: Unassembled WGS sequence
  • UP000077380 Componenti: Unassembled WGS sequence
  • UP000194199 Componenti: Chromosome
  • UP000246245 Componenti: Unassembled WGS sequence
  • UP000095493 Componenti: Unassembled WGS sequence
  • UP000245417 Componenti: Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 342Lyase_1InterPro annotationAdd BLAST331
Domaini408 – 461FumaraseC_CInterPro annotationAdd BLAST54

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni98 – 100Substrate bindingUniRule annotation3
Regioni129 – 132Substrate binding (B site)UniRule annotation4
Regioni139 – 141Substrate bindingUniRule annotation3
Regioni324 – 326Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C9Q Bacteria
COG0114 LUCA

Family and domain databases

CDDicd01362 Fumarase_classII, 1 hit
Gene3Di1.10.275.10, 1 hit
HAMAPiMF_00743 FumaraseC, 1 hit
InterProiView protein in InterPro
IPR005677 Fum_hydII
IPR024083 Fumarase/histidase_N
IPR018951 Fumarase_C_C
IPR020557 Fumarate_lyase_CS
IPR000362 Fumarate_lyase_fam
IPR022761 Fumarate_lyase_N
IPR008948 L-Aspartase-like
PANTHERiPTHR11444 PTHR11444, 1 hit
PfamiView protein in Pfam
PF10415 FumaraseC_C, 1 hit
PF00206 Lyase_1, 1 hit
PRINTSiPR00149 FUMRATELYASE
SUPFAMiSSF48557 SSF48557, 1 hit
TIGRFAMsiTIGR00979 fumC_II, 1 hit
PROSITEiView protein in PROSITE
PS00163 FUMARATE_LYASES, 1 hit

Sequencei

Sequence statusi: Complete.

A0A024L3D9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNTVRSEKDS MGAIDVPADK LWGAQTQRSL EHFRISTEKM PTSLIHALAL
60 70 80 90 100
TKRAAAKVNE DLGLLSEEKA SAIRQAADEV LAGQHDDEFP LAIWQTGSGT
110 120 130 140 150
QSNMNMNEVL ANRASELLGG VRGMERKVHP NDDVNKSQSS NDVFPTAMHV
160 170 180 190 200
AALLALRKQL IPQLKTLTQT LNEKSRAFAD IVKIGRTHLQ DATPLTLGQE
210 220 230 240 250
ISGWVAMLEH NLKHIEYSLP HVAELALGGT AVGTGLNTHP EYARRVADEL
260 270 280 290 300
AVITCAPFVT APNKFEALAT CDALVQAHGA LKGLAASLMK IANDVRWLAS
310 320 330 340 350
GPRCGIGEIS IPENEPGSSI MPGKVNPTQC EALTMLCCQV MGNDVAINMG
360 370 380 390 400
GASGNFELNV FRPMVIHNFL QSVRLLADGM ESFNKHCAVG IEPNRERINQ
410 420 430 440 450
LLNESLMLVT ALNTHIGYDK AAEIAKKAHK EGLTLKAAAL ALGYLSEAEF
460
DSWVRPEQMV GSMKAGR
Length:467
Mass (Da):50,489
Last modified:July 9, 2014 - v1
Checksum:i3D67E3C0F0FDF40B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP021175 Genomic DNA Translation: ARR35196.1
HG976952 Genomic DNA Translation: CDU38545.1
CYBM01000005 Genomic DNA Translation: CTT80252.1
JPQG01000004 Genomic DNA Translation: KFD74733.1
JPKH01000020 Genomic DNA Translation: KGM69889.1
JPKL01000050 Genomic DNA Translation: KGM80316.1
JPMZ01000023 Genomic DNA Translation: KGM82763.1
LEUM01000013 Genomic DNA Translation: KLX97674.1
LDYI01000145 Genomic DNA Translation: KPO06780.1
LIVE01000036 Genomic DNA Translation: KQJ13304.1
LOOZ01000008 Genomic DNA Translation: KXR93459.1
LOGT01000123 Genomic DNA Translation: KYS94007.1
LCVH01000033 Genomic DNA Translation: OAC44124.1
MIWY01000009 Genomic DNA Translation: OEN35164.1
MSEK01000017 Genomic DNA Translation: OLR89053.1
MTKH01000121 Genomic DNA Translation: ONG27786.1
MUAB01000007 Genomic DNA Translation: OUK53685.1
LYBG01000708 Genomic DNA Translation: OWD69422.1
JWSO02000009 Genomic DNA Translation: PNP00863.1
PSSV01000017 Genomic DNA Translation: PPI90419.1
PTOG01000004 Genomic DNA Translation: PPY84333.1
PUJR01000001 Genomic DNA Translation: PQP13321.1
QGJP01000043 Genomic DNA Translation: PWO97519.1
QGJL01000043 Genomic DNA Translation: PWP18881.1
QGJR01000043 Genomic DNA Translation: PWP61787.1
QGJM01000042 Genomic DNA Translation: PWP71970.1
QGJS01000041 Genomic DNA Translation: PWP74139.1
QGJN01000043 Genomic DNA Translation: PWP79419.1
QGJO01000042 Genomic DNA Translation: PWP90830.1
QHMK01000006 Genomic DNA Translation: PXB56449.1
RefSeqiWP_001099085.1, NZ_PYBI01000011.1

Genome annotation databases

EnsemblBacteriaiCDU38545; CDU38545; PGD_01626
CTT80252; CTT80252; ERS085386_01435
KFD74733; KFD74733; JD73_22770
KGM69889; KGM69889; EL75_2048
KGM80316; KGM80316; EL80_2114
KGM82763; KGM82763; EL79_2087
KLX97674; KLX97674; SK85_01828
KPO06780; KPO06780; ACU57_22510
KQJ13304; KQJ13304; AM266_18820
KXQ39797; KXQ39797; AUQ01_11545
KYS94007; KYS94007; AML35_10540
OAC44124; OAC44124; EC3234A_33c01110
OCO42153; OCO42153; AN669_0219525
ODH32858; ODH32858; A6803_01945
OEN35164; OEN35164; BHF46_11765
PATRICifig|562.10472.peg.1568

Similar proteinsi

Entry informationi

Entry nameiA0A024L3D9_ECOLX
AccessioniPrimary (citable) accession number: A0A024L3D9
Entry historyiIntegrated into UniProtKB/TrEMBL: July 9, 2014
Last sequence update: July 9, 2014
Last modified: September 12, 2018
This is version 46 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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