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Signal peptide

Last modified March 12, 2019

This subsection of the 'PTM / Processing' section denotes the presence of an N-terminal signal peptide.

Signal peptides are found in proteins that are targeted to the endoplasmic reticulum and eventually destined to be either secreted/extracellular/periplasmic/etc., retained in the lumen of the endoplasmic reticulum, of the lysosome or of any other organelle along the secretory pathway or to be I single-pass membrane proteins.

The signal sequence is usually removed in the mature protein; in these cases, the comment 'The displayed sequence is further processed into a mature form' is added in the 'Sequence' section.

1. Annotation of experimentally proven signal peptides

We annotate experimentally proven signal peptides when the cleavage site has been determined by direct protein sequencing.
Example: P01165

This information can then be propagated 'By similarity' to closely related species providing the signal sequence is conserved.
Example: Q8CW46

When it is clear that a protein is cleaved (according to experimental data or its similarity with a family of proteins), but direct protein sequencing has not been used to determined the precise cleavage position, we use a question mark instead of a position.
Example: Q9VGW1

In some rare cases, signal sequences are not cleaved. This is indicated by the note 'Not cleaved' in the feature description.
Example: O95445

2. Annotation of predicted signal peptides

We annotate signal peptides which are predicted by the application of the predictive tools Phobius, Predotar, SignalP and TargetP. At least two methods must return a positive signal peptide prediction in order for the prediction to be annotated in UniProtKB. When predicted N-terminal signal peptides and transmembrane regions overlap, then the prediction returned by Phobius is used to discriminate between the two possibilities.
Example: Q6Q788

3. Annotation of Tat signal sequences in bacteria and archaea

This subsection is also used for the annotation of proteins with a Tat (Twin-arginine translocation) signal sequence, which serves to translocate folded proteins in bacteria and archaea. Substrate proteins are directed to the Tat apparatus by distinctive N-terminal signal peptides containing a consensus SRRxFLK 'twin-arginine' motif. The related comment "Predicted to be exported by the Tat system" can be found in the 'Post-translational modification' subsection.
Example: P36649
Predicted TAT signal sequences are identified using the PROSITE profile PS51318.
Predicted cleavage sites are tagged with evidence 'Sequence analysis' or with the automatic annotation rule from which the predicted result was propagated.
Example: P36649

In UniProtKB/TrEMBL, signal peptides are annotated automatically by our sequence annotation module using SignalP.

Related keyword:

See also: Evidence

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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