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Enhanced Y1H assays for Arabidopsis.

Gaudinier A., Zhang L., Reece-Hoyes J.S., Taylor-Teeples M., Pu L., Liu Z., Breton G., Pruneda-Paz J.L., Kim D., Kay S.A., Walhout A.J.M., Ware D., Brady S.M.

We present an Arabidopsis thaliana full-length transcription factor resource of 92% of root stele-expressed transcription factors and 74.5% of root-expressed transcription factors. We demonstrate its use with enhanced yeast one-hybrid (eY1H) screening for rapid, systematic mapping of plant transcription factor-promoter interactions. We identified 158 interactions with 13 stele-expressed promoters, many of which occur physically or are regulatory in planta.

Nat. Methods 8:1053-1055(2011) [PubMed] [Europe PMC]

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