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Seed defensins from T. kiharae and related species: Genome localization of defensin-encoding genes.

Odintsova T.I., Egorov T.A., Musolyamov A.K., Odintsova M.S., Pukhalsky V.A., Grishin E.V.

Ten new defensins have been isolated from seeds of Triticum kiharae and related species of the Triticum and Aegilops genera by a combination of chromatographic procedures including affinity-, size-exclusion, and reversed-phase high-performance liquid chromatography. Nine were completely sequenced and shown to represent a family of closely related peptides with highly conserved amino acid sequences. Analysis of defensin compositions in diploid A-, B-, and D-genome donors to polyploid wheat allowed us for the first time to assign most defensin-encoding genes to particular hexaploid wheat genomes.

Biochimie 89:605-612(2007) [PubMed] [Europe PMC]

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