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Uncovering novel cell cycle players through the inactivation of securin in budding yeast.

Sarin S., Ross K.E., Boucher L., Green Y., Tyers M., Cohen-Fix O.

Budding yeast securin/Pds1p, an inhibitor of the anaphase activator separase/Esp1p, is involved in several checkpoint pathways and in promoting Esp1p's nuclear localization. Using a modified synthetic genetic array (SGA) screen for genes that become essential in the absence of Pds1p, we uncovered roles for uncharacterized genes in cell cycle processes, including Esp1p activation.

Genetics 168:1763-1771(2004) [PubMed] [Europe PMC]

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