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Secondary structure of human granulocyte colony-stimulating factor derived from NMR spectroscopy.

Zink T., Ross A., Ambrosius D., Rudolph R., Holak T.A.

Recombinant 15N-, 13C-labeled human granulocyte colony-stimulating factor (rh-metG-CSF) has been studied by 2D and 3D NMR using uniformly labeled protein as well as residue-specific 15N-labeled samples. Assignment of the 1H, 15N backbone, and 60% 1H sidechain resonances has enabled the determination of the secondary structure of the protein. The secondary structure is dominated by alpha-helical regions with four stretches of helices between residues 11-41, 71-95, 102-124 and 144-170.

FEBS Lett. 314:435-439(1992) [PubMed] [Europe PMC]

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