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Isolation of rat mitochondrial transcription factor A (r-Tfam) cDNA.

Inagaki H., Hayashi T., Matsushima Y., Lin K.H., Maeda S., Ichihara S., Hitagawa Y., Saito T.

We have isolated rat mitochondrial transcription factor A (Tfam; formerly known as mtTFA) cDNA clones from a rat cerebellum cDNA library using human Tfam cDNA as a probe. The deduced amino acid sequence of r-Tfam shows 62% and 89% overall identity to human and mouse Tfam, respectively. We also show the presence of two r-Tfam isoforms in testis as for mouse. Our findings suggest that the mechanisms underlying transcription of mitochondrial genes are conserved among rat, mouse, and human.

DNA Seq. 11:131-135(2000) [PubMed] [Europe PMC]

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