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<td id="blast-threshold">The expectation value (E) threshold is a statistical measure of the number of expected matches in a random database. The lower the e-value, the more likely the match is to be significant. E-values between 0.1 and 10 are generally dubious, and over 10 are unlikely to have biological significance. In all cases, those matches need to be verified manually. You may need to increase the E threshold if you have a very short query sequence, to detect very weak similarities, or similarities in a short region, or if your sequence has a low complexity region and you use the "filter" option</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>E-Thresholdi

<td id="blast-matrix">The matrix assigns a probability score for each position in an alignment. The BLOSUM matrix assigns a probability score for each position in an alignment that is based on the frequency with which that substitution is known to occur among consensus blocks within related proteins. BLOSUM62 is among the best of the available matrices for detecting weak protein similarities. The PAM set of matrices is also available. If "Auto" is set, the matrix will be selected depending on the query sequence length.</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Matrixi

<td id="blast-filter">Low-complexity regions (e.g. stretches of cysteine in <a href="http://www.uniprot.org/uniprot/Q03751">Q03751</a>, or hydrophobic regions in membrane proteins) tend to produce spurious, insignificant matches with sequences in the database which have the same kind of low-complexity regions, but are unrelated biologically. If "Filter low complexity regions" is selected, the query sequence will be run through the program SEG, and all amino acids in low-complexity regions will be replaced by X's. </td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Filteringi

<td id="blast-gapped">This will allow gaps to be introduced in the sequences when the comparison is done.</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Gappedi

Hitsi

Sequence feature

Entry & position(s)Q9RBJ1[1 - 36]
Description
Feature keySignal peptide
Feature identifier
        10         20         30         40         50
MTMTGTVPRS AGWRRLSRRC LPAAFALLFP LGAPHAADTP QWRPVLHFSP
60 70 80 90 100
AAYWMNDPNG PILLDGVYHL FYQYAPGSMT WGHPSWGHAT STDLLHWTEH
110 120 130 140 150
GVAIAATPGE EIFSGSLVPD PLNRSGLGST DAPPLLAFHT SVFHDNPAHP
160 170 180 190 200
DGTQAQSVSV SHDGGFTWRP YAHNPVLTLH PDSRQFRDPS VFWYQDGGCW
210 220 230 240 250
IMTTVVGDAQ LVKLYRSTDL LHWSFLSDFQ PSGYRKPGML WEMPTLVPLK
260 270 280 290 300
LDGNPRATRW VMIVSVNPWS IAGGSGVQYF VGRFDGVTFT PDALPPPGSD
310 320 330 340 350
PSRYDWLDHG ADQYATTLFA NTGSGAPVLI GWMDNWDYAT DLPTAPWRGQ
360 370 380 390 400
MTLPVDIALK TVDGHPTVIQ TPTRAYEDMV RKKGVVTYGD RNLPQDGRVT
410 420 430 440 450
IPSRGEVLDI RLVLRRGGAR RAGIVVRETP DGRTGTSVSY DFVDGTLTVD
460 470 480 490 500
RGRSGLVGFS PRFSTRHIAY LAAPGGEVAL HLVVDRASVE LFANDGVLRM
510 520 530
TDLIFPPAGS DRISLFAEGG GATIHGLRVA VLAR
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Main funding by: National Institutes of Health

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