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<td id="blast-threshold">The expectation value (E) threshold is a statistical measure of the number of expected matches in a random database. The lower the e-value, the more likely the match is to be significant. E-values between 0.1 and 10 are generally dubious, and over 10 are unlikely to have biological significance. In all cases, those matches need to be verified manually. You may need to increase the E threshold if you have a very short query sequence, to detect very weak similarities, or similarities in a short region, or if your sequence has a low complexity region and you use the "filter" option</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>E-Thresholdi

<td id="blast-matrix">The matrix assigns a probability score for each position in an alignment. The BLOSUM matrix assigns a probability score for each position in an alignment that is based on the frequency with which that substitution is known to occur among consensus blocks within related proteins. BLOSUM62 is among the best of the available matrices for detecting weak protein similarities. The PAM set of matrices is also available. If "Auto" is set, the matrix will be selected depending on the query sequence length.</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Matrixi

<td id="blast-filter">Low-complexity regions (e.g. stretches of cysteine in <a href="http://www.uniprot.org/uniprot/Q03751">Q03751</a>, or hydrophobic regions in membrane proteins) tend to produce spurious, insignificant matches with sequences in the database which have the same kind of low-complexity regions, but are unrelated biologically. If "Filter low complexity regions" is selected, the query sequence will be run through the program SEG, and all amino acids in low-complexity regions will be replaced by X's. </td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Filteringi

<td id="blast-gapped">This will allow gaps to be introduced in the sequences when the comparison is done.</td><p><a href='/help/sequence-searches' target='_top'>More...</a></p>Gappedi

Hitsi

Sequence feature

Entry & position(s)A0A381B685[660 - 686]
Description
Feature keyRegion
Feature identifier
        10         20         30         40         50
MPKSVFMRNT AEKDRGNDKR KPFRVSRLIG LDAWIDSALY NLHFRLGEWW
60 70 80 90 100
ENITIFSRRF RVRGFRRFTV EVLDEGFTLG VAGSVLMLML ALPAFEETKK
110 120 130 140 150
DWRAQDDYAV TFLDRYGNEI GRRGILHRQA VPIDELPDHV IKAVLGTEDR
160 170 180 190 200
RFFDHYGIDL MGLSRALSQN MRANGVVQGG STITQQLAKN LFLSNERTIE
210 220 230 240 250
RKVKEAFLAL WLESNLSKKE ILQLYLDRAY MGGGTFGIAA ASEFYFGKNV
260 270 280 290 300
KDISLAEAAM LAGLFKAPAK FAPHVNLPAA RARANVVLSN MVESGFLSEG
310 320 330 340 350
QVAVARRHPA SVIDRAKDES TDYFLDWAFD EVKKVADRFN QHTLIVRTTL
360 370 380 390 400
DRNIQKAAEE SLEFHLRQYG KEYNVSEAAT VVLANDGSVR ALVGGRDYGE
410 420 430 440 450
SQFNRATRAL RQAGSSFKPY VYAAAMEKGL TPSTIVSDAP ISWGNWSPRN
460 470 480 490 500
YGRSFAGRVD LTTALVRSLN SVPVRLARDY LTTAPVVALT KAMGVESPIS
510 520 530 540 550
SHKTMVLGTS EMTVMDQATG FNVFANAGMA GNRHAFTQIL ASDGKVLWDF
560 570 580 590 600
GRDAPKPHRA LSEKAALEMN SMLVQVPERG TGRRAALTMT RVAGKTGTTQ
610 620 630 640 650
NYRDAWFVGF TGNFTAAVWF GNDNFTPMKE LTGGVLPAMA WQRMMAYAHQ
660 670 680 690 700
NIELKPLPGV NPPFPAQPKN PPAQVADTRP HETMAAPPRV LSPLATKILK
710 720
ELHDRFLAAP PLPTIAERTK VSVL
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Main funding by: National Institutes of Health

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