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The annotation and conditions in this rule are derived from the following entries: P0A814 (RUVC_ECOLI)

If a protein meets these conditions... i

Common conditions

    • Matches HAMAP signature MF_00034
    • taxon = Bacteria
    • fragment ≠ the sequence is fragmented

Special conditions

    • Subsequence at position 8 - 8 aligns to "D" in entry P0A814 Subsequence at position 67 - 67 aligns to "E" in entry P0A814 Subsequence at position 139 - 139 aligns to "[DH]" in entry P0A814 Subsequence at position 142 - 142 aligns to "D" in entry P0A814
  • Subsequence at position 8 - 8 aligns to "D" in entry P0A814 (applies "Magnesium") Subsequence at position 67 - 67 aligns to "E" in entry P0A814 (applies "Magnesium") Subsequence at position 139 - 139 aligns to "[DH]" in entry P0A814 (applies "Magnesium") Subsequence at position 142 - 142 aligns to "D" in entry P0A814 (applies "Magnesium")

... then these annotations are applied i

Protein namei

  • Recommended name:
    Crossover junction endodeoxyribonuclease RuvC (EC:3.1.22.4)
    Alternative name(s):
    Holliday junction nuclease RuvC
    Holliday junction resolvase RuvC

Gene namei

  • Name:ruvC

Sequence similaritiesi

Cofactori

  • Mg2+Note: Binds 1 Mg2+ ion per subunit.

Functioni

  • Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.

Catalytic activityi

  • Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).

Metal bindingi

  • Magnesium (to residues corresponding to position 8)
  • Magnesium (to residues corresponding to position 67)
  • Magnesium (to residues corresponding to position 139)
  • Magnesium (to residues corresponding to position 142)

Keywordsi

GO (Gene Ontology) termsi